rev: March 6, 2005
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CYTOKERATIN
ANTIBODIES
(anti-Human and others as indicated)
RDI Divison of researchd Industries Intl offers a wide line of antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.
ANTI-CYTOKERATIN (PAN EPITHELIAL)
also available biotin conjugated cat#RDI-PRO61531 $469.00/0.25ml
also available FITC conjugated cat#RDI-PRO61431 $469.00/0.25ml
Clone Determination
Ks 5+8.22/C 22
Category
Mouse
monoclonal
Immunoglobulin Class
IgG1
Purification
Ion-exchange
chromatography
Antigen
Human cytokeratin 8, purified from SDS PAGE
Specificity C 22 represents an excellent marker for distinguishing CA from all non-epithelial tumors
Application
Suitable for ·
frozen and paraffin-embedded tissue· cytological material
· Immunoblotting (Western) :Flow cytometry
Stability at 4°C
one year after
reconstitution
Quantity
50 µg
(lyoph.)
Polypeptide Reacting MR 52 500, MR 58 000 cytokeratins (type II cytokeratins nos. 5+8)
of human epithelial cells. Epitope has been mapped to aa353-367 on alpha
helical rod domain
Antigen Recognized in Species
(tested so far)
Human, bovine, murine, amphibia, pig,
hydra
Reactivities on Cultured
Cell lines (tested so far) MCF-7, RT 112, HT-29,DEtroit 562, RPMI 2650, SSC-12
bovine BMGE+H, BMGE-H, MDBK
Tumors Specifically Detected All epithelium-derived neoplasms
Pretreatment With paraffin embedded sections, protease pretreatment is required
prior to antibody application
Working Dilution When reconstituted with 1ml distilled water, dilute further 1:10 Incubation Time 1h at RT, extended with P
(over night at 4°C)
References
Bartek J., Vojtesek B., Staskova Z., Bartkova J., Kerekes Z., Rejthar A. and Kovarik J.: A series of 14 new monoclonal antibodies to keratins: Characterization and value in diagnostic histopathology. J. Pathol. 164: 215-224 (1991)
Heid, H., Vojtesek, B., Bruder, G., Kaufman, M., Staskova, Z., Kovarik, J., Bartek, J. and Franke W.W.: Cell type heterogeneity of mammary gland epithelial and breast carcinoma cells as revealed by cytokeratin expression. Differentiation (1991), submitted
Moll, R., Franke, W.W., Schiller, D.L., Geiger, B. and Krepler, R.: The catalog of human cytokeratins: Patterns of expression in normal epithelia, tumors and cultured cells. Cell 31, 11-24 (1982)
For research use only. Not supplied for use in human diagnostic or therapeutic
procedures
Cat#RDI-PRO61531 $469.00
Clone Determination Ks 5+8.22/C 22
Category Mouse monoclonal, biotin conjugate
Immunoglobulin Class IgG 1
Purification Protein A affinity chromatography
Antigen Human cytokeratin 8, purified from SDS PAGE
Description/Specificity C 22 represents an excellent marker for distinguishing carcinomas from all non-epithelial tumors. The antibody specifically reacts with cytokeratins 5 and 8 present in nearly all epithelia. Polypeptide reacting:Mr 52 500, Mr 58 000 cytokeratins (type II cytokeratins nos. 5+8) of human epithelial cells. Epitope has been mapped to aa353-367 on alpha helical rod (See Waseem et al, 2004)
Antigen Recognized in Human, bovine, murine, amphibia, pig, hydra
Species (tested so far)
Reactivities on Cultured MCF-7, RT 112, HT-29, Detroit 562, RPMI 2650,
Cell lines (tested so far) SSC-12, bovine BMGE+H, BMGE-H, MDBK
Application Suitable for
· frozen and paraffin-embedded tissue and cytological material in immunohistochemistry
· FACS
· ELISA
Storage Store at 2-8°C. For extended storage keep in aliquots at -20°C. Avoid repeated thawing and freezing.
Pretreatment With paraffin embedded sections, protease pretreatment is required prior to antibody application
Working Dilution 1:10 - 1:20 for immunohistochemical application
Incubation Time 1h at RT, extended with paraffin sections (overnight at 2-8°C)
Quantity 250 µL
References
Bartek J., Vojtesek B., Staskova Z., Bartkova J., Kerekes Z., Rejthar A. and Kovarik J.: A series of 14 new monoclonal antibodies to keratins: Characterization and value in diagnostic histopathology. J. Pathol. 164: 215-224 (1991)
Heid, H., Vojtesek, B., Bruder, G., Kaufman, M., Staskova, Z., Kovarik, J., Bartek, J. and Franke W.W.: Cell type heterogeneity of mammary gland epithelial and breast carcinoma cells as revealed by cytokeratin expression. Differentiation (1991),
Moll, R., Franke, W.W., Schiller, D.L., Geiger, B. and Krepler, R.: The catalog of human cytokeratins: Patterns of expression in normal epithelia, tumors and cultured cells. Cell 31, 11-24 (1982)
Waseem, a et al, Biochemistry 43, 1283-1295 (2004)
For Research Use Only http://www.researchd.com/absort.htm
RDI Divison of researchd Industries Intl
San Jose, 95123 CA Snell ave 658
USA
or 408-780-0908
EMAIL:margaret@cellular-research.com
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