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CYTOKERATIN   ANTIBODIES  

(anti-Human and others as indicated)

RDI Divison of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.


ANTI-CYTOKERATIN (PAN EPITHELIAL)


Mab to Pan Epithelial Cytokeratin 5/8   cat#RDI-PRO61031 $375.00/50ug vial

also available biotin conjugated cat#RDI-PRO61531 $469.00/0.25ml

also available FITC conjugated cat#RDI-PRO61431 $469.00/0.25ml

Clone Determination                       Ks 5+8.22/C 22

Category                                        Mouse monoclonal

Immunoglobulin Class                     IgG1

Purification                                     Ion-exchange chromatography

Antigen                                          Human cytokeratin 8, purified from SDS PAGE

Specificity                                     C 22 represents an excellent marker for distinguishing CA from all non-epithelial tumors


Application                                  Suitable for · frozen and paraffin-embedded tissue· cytological material  

                                                  · Immunoblotting (Western)  :Flow cytometry

Stability at 4°C                           one year after reconstitution

Quantity                                     50 µg (lyoph.)

Polypeptide Reacting                 MR 52 500, MR 58 000 cytokeratins (type II cytokeratins nos. 5+8)

                                                of human epithelial cells. Epitope has been mapped to aa353-367 on alpha

                                                helical rod domain

Antigen Recognized in Species

(tested so far)                            Human, bovine, murine, amphibia, pig, hydra


Reactivities on Cultured

Cell lines (tested so far)           MCF-7, RT 112, HT-29,DEtroit 562, RPMI 2650, SSC-12  

                                               bovine BMGE+H, BMGE-H, MDBK

Tumors Specifically Detected All epithelium-derived neoplasms


Pretreatment With paraffin embedded sections, protease pretreatment is required prior to antibody application

Working Dilution When reconstituted with 1ml distilled water, dilute further 1:10 Incubation Time 1h at RT, extended with P

(over night at 4°C)


References

Bartek J., Vojtesek B., Staskova Z., Bartkova J., Kerekes Z., Rejthar A. and Kovarik J.: A series of 14 new monoclonal antibodies to keratins: Characterization and value in diagnostic histopathology. J. Pathol. 164: 215-224 (1991)

Heid, H., Vojtesek, B., Bruder, G., Kaufman, M., Staskova, Z., Kovarik, J., Bartek, J. and Franke W.W.: Cell type heterogeneity of mammary gland epithelial and breast carcinoma cells as revealed by cytokeratin expression. Differentiation (1991), submitted

Moll, R., Franke, W.W., Schiller, D.L., Geiger, B. and Krepler, R.: The catalog of human cytokeratins: Patterns of expression in normal epithelia, tumors and cultured cells. Cell 31, 11-24 (1982)


For research use only. Not supplied for use in human diagnostic or therapeutic procedures


Mab to Cytokeratin 5/8 Pan Epithelial Cytokeratin Biotin Conjugate

Cat#RDI-PRO61531  $469.00

Clone Determination Ks 5+8.22/C 22

Category Mouse monoclonal, biotin conjugate

Immunoglobulin Class IgG 1

Purification Protein A affinity chromatography

Antigen Human cytokeratin 8, purified from SDS PAGE

Description/Specificity C 22 represents an excellent marker for distinguishing carcinomas from all non-epithelial tumors. The antibody specifically reacts with cytokeratins 5 and 8 present in nearly all epithelia. Polypeptide reacting:Mr 52 500, Mr 58 000 cytokeratins (type II cytokeratins nos. 5+8) of human epithelial cells. Epitope has been mapped to aa353-367 on alpha helical rod (See Waseem et al, 2004)

Antigen Recognized in Human, bovine, murine, amphibia, pig, hydra

Species (tested so far)

Reactivities on Cultured         MCF-7, RT 112, HT-29, Detroit 562, RPMI 2650,

Cell lines (tested so far)          SSC-12, bovine BMGE+H, BMGE-H, MDBK

Application Suitable for

· frozen and paraffin-embedded tissue and cytological material in immunohistochemistry

· FACS

· ELISA

Storage Store at 2-8°C. For extended storage keep in aliquots at -20°C. Avoid repeated thawing and freezing.

Pretreatment With paraffin embedded sections, protease pretreatment is required prior to antibody application

Working Dilution 1:10 - 1:20 for immunohistochemical application

Incubation Time 1h at RT, extended with paraffin sections (overnight at 2-8°C)

Quantity 250 µL

References

Bartek J., Vojtesek B., Staskova Z., Bartkova J., Kerekes Z., Rejthar A. and Kovarik J.: A series of 14 new monoclonal antibodies to keratins: Characterization and value in diagnostic histopathology. J. Pathol. 164: 215-224 (1991)

Heid, H., Vojtesek, B., Bruder, G., Kaufman, M., Staskova, Z., Kovarik, J., Bartek, J. and Franke W.W.: Cell type heterogeneity of mammary gland epithelial and breast carcinoma cells as revealed by cytokeratin expression. Differentiation (1991),

Moll, R., Franke, W.W., Schiller, D.L., Geiger, B. and Krepler, R.: The catalog of human cytokeratins: Patterns of expression in normal epithelia, tumors and cultured cells. Cell 31, 11-24 (1982)

Waseem, a et al, Biochemistry 43, 1283-1295 (2004)

For Research Use Only http://www.researchd.com/absort.htm


RDI Divison of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

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