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CYTOKERATIN ANTIBODIES
(anti-Human and others as indicated)
RDI Division of Fitzgerald Industries Intl offers a wide line of antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.
ANTI-CYTOKERATIN 19
Mab to Cytokeratin 19
cat#RDI-PRO61029, new cat#: 10R-C162a $412.50/vial
Clone Determination Ks 19.2 (Z105.6)
Category Mouse monoclonal
Immunoglobulin Class IgG2b
Purification SpAss Protein A (recombinant)
Antigen Cytokeratin 19 of Mr 40000; from cultured human MCF-7 cells
Specificity Ks 19.2 represents an excellent marker to discriminate glandular
epithelial Ca. from those of different origin. No reaction with hepatocellular Ca.!
Application Suitable for frozen tissue
Cytological material
Immunoblotting (Western)
ELISA
Storage At 4°C
Quantity 50 µg (lyoph.) ; contains 0.5% BSA and
0.09% NaN3
Polypeptide Reacting Mr 40 000 polypeptide (cytokeratin No. 19) of human
glandular epithelia
Antigen Recognized in Species tested so far: Human, rat, fish, bovine; not with mouse, woodchuck, chicken, xenopus
Reactivities on Cultured Cell lines (tested so far)
MCF-7, RT 112,
Tumors Specifically Detected All tested adeno Ca.; cholangio Ca. of liver; renal cell Ca.; transitional cell Ca. of the bladder; ovary Ca.; squamous cell Ca. of cervix, bronchus and lung (inter-mediate type); mesothelioma; carcinoid tumor of bronchus; breast Ca.; thymoma
Working Dilution After reconstitution with 1 ml dist. water, dilute 1:10 with
PBS, pH 7.4 for use in immunohistochemistry
Incubation Time 1 h at RT
References
Bader, B.L. and Franke, W.W.: Celltype-specific and efficient synthesis of human cytokeratin 19 in transgenic mice. D differentiation 45, 109-118 (1990)
Bader, B.L., Jahn, L. and Franke, W.W.: Low level expression of cytokeratins 8,
18 and 19 in vascular smooth muscle cells of human umbilical cord and in
cultured cells derived therefrom, with an analysis of the chromosomal locus
containing the cytokeratin 19 gene. Eur. J. Cell Biol. 47, 300-319 (1988)
Bosch, F.X., Ouhayoun, J.-P., Bader, B.L., Collin, C., Grund, C., Lee,
Bosch, F.X., Leube, R.E., Achtstätter, T., Moll, R. and Franke, W.W.:
Expression of simple epithelial type cytokeratins in stratified epithelia as
detected by immunolocalization and hybridization in situ. J. Cell Biol. 106,
1635-1648 (1988)
Dockhorn-Dworniczak, B., Franke, W.W., Schröder, S., Czernobilsky, B.,
Gould, V.E. and Böcker, W.: Patterns of expression of cytoskeletal
proteins in human thyroid gland and thyroid carcinomas. Differentiation 35,
53-71 (1987)
Heid, H.W., Moll,
Heid, H.W., Moll.
Moll, R., Franke, W.W., Schiller, D.L., Geiger, B. and Krepler, R.: The catalog
of human cytokeratins: Patterns of expression in normal epithelia, tumors and
cultured cells. Cell 31, 11-24 (1982)
Moll,
Moll,
Moll, R., Schiller, D.L. and Franke, W.W.: Identification of protein IT of the
intestinal cytoskeleton as a novel type I cytokeratin with unusual properties
and expression patterns. J. Cell Biol. 111, 567-580 (1990)
For research use only. Not supplied for use in human diagnostic or therapeutic procedures
RDI Division of Fitzgerald Industries Intl
phone (800) 370-2222 or (201) 584-7093
fax (978) 371-2266
EMAIL:antibodies@fitzgerald-fii.com
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