rev: January 27, 2005
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(anti-Human and others as indicated)
RDI Divison of researchd Industries Intl offers a wide line of antibodies. Since no one antibody works best for all applications (neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.
ANTI-VIMENTIN (see 2 monoclonals and 1 polyclonal below)
see also vimentin antigen
MONOCLONAL ANTIBODY TO VIMENTIN
PRODUCT CODE :RDI-PRO61013 $345.00/vial
also available FITC labeled: cat#RDI-PRO61413 $469.00/0.25ml
Biotin labeled: cat#RDI-PRO61513
CLONE: VIM 3B4
SOURCE: Mouse ascitic fluid.
PURIFICATION METHOD: Protein A affinity chromatography
SPECIFICITY: All cells shown to be positive with antisera to vimentin were also positive with this antibody: cells of mesenchymal derivation, including endothelial cells and certain smooth muscle cells of the vascular tract, fibroblasts, connective tissue, all types of blood cells, including thymocytes, interstitial and Sertoli-cells of testis, ovarian follicle cells; detects coexpression of vimentin in combination with other intermediate filament proteins. Specific for vimentin (Mr 57,000 on SDS-PAGE) recognized from the following species. Human, bovine, chicken, amphibia (Xenopus).
-The binding region of monoclonal antibody VIM3B4 has been characterized by Bohn et al.(1992). According to these authors, the epitope has been localized on the alpha-helical part of vimentin (rod domain coil 2). Due to an aa substitution at position of aa 353 in murine vimentin (that could explain for the weak cross-reaction of the antibody with murine vimentin) they were able to narrow down the binding region around position 353. These findings were confirmed by truncation mutagenesis experiments using human vimentin (Rogers et al., 1995).
APPLICATIONS: * RD cells, glioma cells, fibroblasts (SV-80, 3T3), BHK
* All vimentin expressing tumours such as sarcomas (including myosarcomas), lymphomas, melanomas etc.
* The antibody is brilliantly positive on sections
of frozen tissues and on formalin-fixed,
paraffin-embedded tissue samples. In comparative analyses, VIM 3B4 has turned
out to be the
monoclonal antibody to vimentin.
REFERENCES: Franke, W.W. & Moll, R. Differentiaition 36, 145 - 63 (1987)
Heid, H.W. et al. Differntiation 37, 137 - 57 (1988)
Kasper, M. et al. Histochem. 93,
93 - 103 (1989)
PRESENTATION: Each monoclonal is delivered in a sealed vial at a concentration of 50µg lyophilysed. Reconstitute with 1ml distilled water. We recommend that each laboratory determine an optimum working titre for use in its particular application.
STORAGE: Purified monoclonal antibodies are generally stable for long periods at 4oC. However repeated warming to room temperature and re-cooling may result in a loss of activity and hence effective working titre. It is recommended that monoclonal antibodies are aliquotted upon receipt and then stored at -200C. After thawing they should then be used as a stock solution for up to 60 days. Dilute working solutions are best prepared on the day of use.
For research use only. Not supplied for use in human diagnostic or therapeutic procedures
Source Guinea pig
Form Whole antiserum
Antigen Vimentin purified from calf lens
Reactive Polypeptide(Specificity) : Specific detection of vimentin (Mr 57000 polypeptide)
Antigen Recognized in Species (tested so far): Human, bovine,
hamster, mouse, Xenopus
Tumors Specifically Detected Sarcoma (including myosarcoma), lymphoma, melanoma
Application Suitable for
· immunohistochemistry on frozen* and paraffin- embedded** (P) tissue
· cytological material
· immunoblotting*** (Western)
Working Dilution * 1:50 for immunohistochemistry
** 1:25 for paraffin-embedded tissue (Protease-pretreatment)
*** 1:500 for Western blotting
Incubation Time 1 h at RT; extended with P
Stability/Storage At 4°C min. one year
Volume 100 µl; contains 0.09% sodium azide
For research use only, not for therapeutic or diagnostic use!
Category Mouse monoclonal
Ig Subclass IgG1
Purification/Form Hybridoma culture supernatant
Antigen Vimentin from cytoskeletal fraction of XLKE cells ( cultured Xenopus laevis kidney epithelial cells)
Clinical Application/ Specificity: Vimentin of amphibia and fish, predominantly in glial and white blood cells
Application · Western blotting
· Immunohistochemistry on frozen sections
Polypeptide Reacting MW 53,325 (pI 4.95) intermediate filament protein (vimentin) of Xenopus laevis (epitope presumably located between amino acids 79 and 88 within rod domain)
Antigen Recognized Xenopus laevis, rainbow trout (Oncorhynchus in Species (tested so far) mykiss), carp; human and bovine are negative
Working Dilution Ready-to-use for immunohistochemistry
Dilution Buffer 0.15 M phosphate buffered saline with 0.1% BSA and 0.1% Na-Azide
Storage at 4°C
Volume 5 ml
Herrmann H., Münick M.D., Brettel M., Fouquet B. and Markl J. (1995): Vimentin in a cold-water fish, the rainbow trout: highly conserved primary structure but unique assembly properties. J. Cell Sc. 109:569-578 (1996).
For Research Use Only
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RDI Divison of researchd Industries Intl
San Jose, 95123 CA Snell ave 658