rev: August 3, 2004
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CYTOKERATIN
ANTIBODIES
(anti-Human and others as indicated)
RDI Divison of researchd Industries Intl offers a wide line of antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.
ANTI-CYTOKERATIN TYPE II
Mab to Cytokeratin Type II
cat#RDI-PRO61006 $344.00/vial 50ug purified
Also available:Biotin labeled cat#RDI-PRO61506 $469.00/0.25ml (approx 1.2mg/ml)
FITC conjug cat#RDI-PRO61406 $469.00/0.25ml
-both suitable for flow cytometry, histochemistry and cytochemistry
Clone Determination Ks pan 1-8
Category
Mouse monoclonal
Immunoglobulin Class IgG2a
Purification
SpAss-Protein A (recombinant)
Antigen Cytoskeletal proteins from cultured human MCF-7 cells
Specificity Ks pan 1-8 represents an excellent marker for distinguishing CA from non-epithelial tumors
Application Suitable for · frozen tissue
· immunoblotting (Western)
· cytological Material
Stability/Storage One year after reconstitution when stored at 4°C
Quantity 50 µg (lyoph.) contain 0.5% BSA and 0.09% NaN3
Polypeptide Reacting MR 52 500 -MR 68,000 cytokeratins (type II cytokeratins 1-8) of human epithelial cells
Antigen Recognized in species tested so far: Human, bovine, amphibia
Reactivities on Cultured Cell lines tested so far : MCF-7, RT 122,
HT-29, Detroit 562, RPMI 2650, SSC-12, bovine BMGE+H, BMGE-H, MDBK
Structures and Tissues Specificallly detected ; All epithelium-derived
neoplasms
Working Dilution When reconstituted with 1ml distilled water dilute 1:10
Incubation: 1HR RT
References
1) Achstatter, Th et al, Cytokeratin filaments and desmosomes in the epithelial cells of the perineurial and arachnoidal sheaths of some verterbrate species. Differentiation 40:129-149 (1989)
2) Gould, VE et al:Coexpression patterns of vimentin and glial filament protein with cytokeratin in the normal, hyperplastic and neoplastic breast, AM. J. Pathol 137:1143-1155 (1989)
3) Heid, H.W. et al, Patterns of expression of trichocytic and epithelial cytokeratins in mammalian tissues, I. Human and bovine hair follicles. Differentiation 37:137-157 (1988)
4) Moll, R et al:The catalog of human cytokeratins:Patterns of expression in normal epithelia, tumors and cultured cells. Cell 31, 11-24 (1982
5) Moll. R et al:Identification of protein IT of the intestinal cytoskeletan as a novel type I cytokeratin with unusual properties and expresion patterns. Journal Cell Biol 111, 587-580 (1990
For In Vitro Research Use Only
Larger sizes quoted on request
For research use only. Not supplied for use in human diagnostic or therapeutic
procedures
San Jose, 95123 CA Snell ave 658
USA
or 408-780-0908
EMAIL:margaret@cellular-research.com
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