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CYTOKERATIN   ANTIBODIES  

(anti-Human and others as indicated)

RDI Divison of researchd Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.

ANTI-CYTOKERATIN TYPE II

Mab to Cytokeratin Type II

cat#RDI-PRO61006    $344.00/vial 50ug purified

Also available:Biotin labeled cat#RDI-PRO61506   $469.00/0.25ml (approx 1.2mg/ml)

                         FITC conjug   cat#RDI-PRO61406   $469.00/0.25ml

-both suitable for flow cytometry, histochemistry and cytochemistry

Clone Determination          Ks pan 1-8

Category                            Mouse monoclonal

Immunoglobulin Class        IgG2a

Purification                        SpAss-Protein A (recombinant)

Antigen                              Cytoskeletal proteins from cultured human MCF-7 cells

Specificity                         Ks pan 1-8 represents an excellent marker for distinguishing CA from non-epithelial tumors

Application Suitable for   · frozen tissue

                                          · immunoblotting (Western)

                                          · cytological Material

Stability/Storage                One year after reconstitution when stored at 4°C

Quantity                             50 µg (lyoph.) contain 0.5% BSA and 0.09% NaN3

Polypeptide Reacting        MR 52 500 -MR 68,000 cytokeratins (type II cytokeratins 1-8) of human epithelial cells

Antigen Recognized in species tested so far: Human, bovine, amphibia

Reactivities on Cultured Cell lines tested so far :  MCF-7, RT 122, HT-29, Detroit 562, RPMI 2650, SSC-12, bovine BMGE+H, BMGE-H, MDBK

Structures and Tissues Specificallly detected ; All epithelium-derived neoplasms

Working Dilution When reconstituted with 1ml distilled water dilute 1:10

Incubation: 1HR RT

References

1) Achstatter, Th et al, Cytokeratin filaments and desmosomes in the epithelial cells of the perineurial and arachnoidal sheaths of some verterbrate species. Differentiation 40:129-149 (1989)

2) Gould, VE et al:Coexpression patterns of vimentin and glial filament protein with cytokeratin in the normal, hyperplastic and neoplastic breast, AM. J. Pathol 137:1143-1155 (1989)

3) Heid, H.W. et al, Patterns of expression of trichocytic and epithelial cytokeratins in mammalian tissues, I. Human and bovine hair follicles. Differentiation 37:137-157 (1988)

4) Moll, R et al:The catalog of human cytokeratins:Patterns of expression in normal epithelia, tumors and cultured cells. Cell 31, 11-24 (1982

5) Moll. R et al:Identification of protein IT of the intestinal cytoskeletan as a novel type I cytokeratin with unusual properties and expresion patterns. Journal Cell Biol 111, 587-580 (1990

For In Vitro Research Use Only

Larger sizes quoted on request


For research use only. Not supplied for use in human diagnostic or therapeutic procedures

RDI Divison of researchd Industries Intl

San Jose, 95123 CA Snell ave 658

USA

or 408-780-0908

EMAIL:margaret@cellular-research.com

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