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CYTOKERATIN   ANTIBODIES  

(anti-Human and others as indicated)

RDI Division of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.


ANTI-CYTOKERATIN  TYPE 19

-see other clones and polyclonal from main cytokeratin page


Mab to Human Cytokeratin 19

Cat#RDI-PRO11417    $375.00

Clone              RCK 108

Category         Mouse monoclonal

Form/Purification Culture supernatant

Ig Class           IgG1

Immunogen     Human bladder cell line T24

Description/Specificity Mab RCK 108 specifically reacts with cytokeratin 19(40 kD protein in immunoblotting), found in mostglandular simple type epithelia.

Antigen Recognized        Human, most mammals (incl. mouse and rat)

in Species (tested so far)

Positive Control               Glandular epithelia

Application o Immunofluorescence

                    o Immunohistochemistry

                    o Immunoblotting

                   o Suitable for frozen and paraffin sections No proteolytic pretreatment required. Optimal results are obtained when using the microwave retrieval method in citrate buffer.

Dilution Buffer PBS with 1% BSA and 0.1% Na-Azide

Working Dilution 1:10 - 1:20 for immunohistochemistry

Storage At 2-8°C (undiluted) or in aliquots at -20°C

Volume 1ml

References Kwaspen FH, Smedts FM, Broos A, Bulten H, Debie WM, Ramaekers FCS: Reproducible and highly sensitive detection of the broad spectrum epithelial marker keratin 19 in routine cancer diagnosis. Histopathology 31: 503-516 (1997).

Schaafsma HE, Ramaekers FCS: Cytokeratin subtyping in normal and neoplastic epithelium: basic principles and diagnostic applications. Pathol Ann 29: 21-62 (1994).

Smedts F, et al. Changing Patterns of keratin expression during progression of cervical intraepithelial neoplasia. Am J Pathol 136:657-668 (1990).

For In Vitro research Use only


RDI Division of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

Ordering terms

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