rev: February 2, 2005
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CYTOKERATIN & RELATED
ANTIBODIES
(anti-Human and others as indicated)
RDI Divison of Fitzgerald Industries Intl offers a wide line of antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.
cat#RDI-PRO10765 $375.00/vial
Category Rabbit, polyclonal antiserum
Ig Class IgG
Immunogen Laminin isolated from Engelbrecht-Holm-Swarm (EHS)-mouse sarcoma
Introduction Laminin is a glycoprotein (Mr 850 - 1.000 kDa, consisting of 3 glycosylated polypeptide chains with molecular weights of 440 and 225 (2x) kDa) produced by various human epithelial and mesenchymal cells, and forms an extracellular matrix of thin filaments. In normal tissues, laminin is invariably present in all basal laminas surrounding muscle, nerve, fat and decidua cells and separates epithelial and endothelial cells from abutting connective tissues. Laminin has also been identified within the cytoplasm of breast epithelia, stromal cells of the endometrium, and within endothelial, bile duct epithelial and mesenchymal cells of the liver. Laminin has been found to be involved in cellular activities such as adhesion, spreading, differentiation, polarization, proliferation, locomotion, tissue invasion and chemotactic responses.
Clinical Application/ Specificity The antibody reacts with a 200 kDa protein band in immunoblotting. No cross reaction was obtained with human type I, III, IV and V collagen in immunohistochemstry.
Recommended Positive Control: for Skin, kidney
Application ELISA
Immunoblotting
Immunohistochemistry
Suitable for frozen tissue and paraffin* sections (proteolytic treatment required; enzyme: pepsin)
Antigen Recognized :Human, mouse, rat, rabbit, bovine, pig
Working Dilution 1:50 - 1:100
Dilution Buffer PBS with 1% BSA and 0.1% Na-Azide
Incubation Time 1 h at room temperature
Storage At 4°C (undiluted) or at -20°C (aliquots)
Volume 0,25 ml
References
Lise Christensen: The distribution of fibronectin, laminin and tetranectin in human breast cancer with special attention to the extracellular matrix. APMIS Supplementum No. 26, Vol. 100 (1992).
Bosman, F.T et al. Basement Membranes in Cancer. Ultrastructural Pathology 8:291-304 (1985)
see also cat#RDI-LAMINabr $469.00/500ug suitable for western blotting, Elisa, histochemistry (<1% cross with other extracellular matrx proteins). Reacts with most mammalian laminins. Also availabel Biotin labeled, cat#RDI-LAMINabrBT $312.00/100ug
For research use only. Not supplied for use in human diagnostic or therapeutic procedures
PRODUCT: Affinity Purified Anti-Laminin (Rabbit) Minimum Cross Reaction to collagens
CODE NO.: RDI-LAMINabr $594.00/vial X=$344.00/100ug
also available Biotin labeled cat#RDI-LAMINabrBT $344.00/100ug
PHYSICAL STATE: Liquid (Sterile Filtered)
SIZE: 0.5 mg (0.42ml)
ANTIBODY CONCENTRATION: 1.2 mg/ml (by UV absorbance at 280 nm)
BUFFER: 0.125M Sodium Borate, 0.075M Sodium Chloride, 0.005MEDTA; pH 8.0
STABILIZER: None
PRESERVATIVE: 0.01% NaN3 (sodium azide)
RECOMMENDED DILUTIONS: Western Blots 1:5,000 - 1:10,000
ELISA 1:4,000 - 1:8,000
Immunohistochemistry 1:50 - 1:200
APPLICATIONS: ELISA, Western Blotting (non reduced), Immunochemistry.
STORAGE: Store vial at 4`C before opening. Avoid cycle of thawing and freezing. Dilute only prior to using. For extended storage mix with an equal volume of glycerol, aliquot contents and store at -20 DEG C.
PURITY: This product has been prepared by immunoaffinity chromatography using immobilized antigens. Extensive cross-absorptionwas performed against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwantedspecificities. Typically less than 1% cross reactivity against othertypes of collagen was detected using ELISA. S
SPECIFICITY: Antibody reacts with most mammalian laminin proteins
IMMUNOGEN: non-reduced 400kda human Laminin
NOTE: This product is FOR IN-VITRO Research USE ONLY
PRODUCT: Affinity Purified Anti-Laminin (Rabbit) Minimum Cross Reaction to collagens
CODE NO.: RDI-LAMINabrBT $344.00/vial
LOT NO:
PHYSICAL STATE: Liquid (Sterile Filtered)
SIZE: 0.1 mg (0.1ml)
ANTIBODY CONCENTRATION: 1.0 mg/ml (by UV absorbance at 280 nm)
BUFFER: 0.2M potassium phosphate,0.15M Sodium ChloridepH7.2
STABILIZER: 10mg/ml BSA
PRESERVATIVE: 0.01% NaN3 (sodium azide)
RECOMMENDED DILUTIONS: Western Blots 1:5,000 - 1:10,000
ELISA 1:4,000 - 1:8,000
Immunohistochemistry 1:50 - 1:200
APPLICATIONS: ELISA, Western Blotting (non reduced), Immunochemistry.
STORAGE: Store vial at 4`C before opening. Avoid cycles of thawing and freezing. Dilute only prior to using. For extended storage mix with an equal volume of glycerol, aliquot contents and store at -20 DEG C.
PURITY: This product has been prepared by immunoaffinity chromatography using immobilized antigens. Extensive cross-absorption was performed against other collagens, human serum proteins and non- collagen extracellular matrix proteins to remove any unwanted specificities. Typically less than 1% cross reactivity against other types of collagen was detected using ELISA. S
SPECIFICITY: Antibody reacts with most mammalian laminin proteins
IMMUNOGEN: non-reduced 400kda human Laminin
NOTE: This product is FOR IN-VITRO Research USE ONLY
cat# RDI-MLAMININ-Ag $469.00/2mg
Presentation: 2mg in 2.1ml of 0.05M Tris-HCl, pH 7.4, 0.15M NaCl. Frozen solution.
Source: Purified from basement membrane of the Engelbreth-Holm-Swarm (EHS) mouse tumor using salt precipitation and ion-exchange chromotography.
Use: Thaw frozen product at 4 C and keep on ice thereafter. Dilute with sterile buffer or serum-free medium to approximately 20 g/ml. Add directly to culture vessels to achieve 2.5-10 g/cm 2 . Allow the matrix proteins to adsorb to the vessel for one hour at 37 C or overnight at 4 C prior to removing and adding medium.
Sterility: This lot of laminin was tested for the presence of bacteria, fungi and mycoplasma.
Storage: Stable for 1 year at -70 C from date of shipment. Aliquot to avoid repeated freezing and thawing by dispensing into appropriate aliquots if the material is not to be used all at once. Dilute in any physiological buffer or culture medium.
FOR RESEARCH USE ONLY-Not for use in or humans or animals NOT FOR USE IN HUMANS
Quality Control Testing
Attachment Assay: This lot of laminin was tested for the ability to promote cell attachment using PC12 rat pheochromocytoma cells in an attachment assay. The optimal range was found to be 2.5-10 g/cm 2 . Laminin Attachment Assay Protocol
1. Coat 6 well dishes with laminin, at a concentration of 2.5-10 g/cm
2 . Incubate 1 hour at 37 C or overnight at 4 C. Aspirate and rinse 2 times with PBS before adding cell suspension.
3. Prepare PC-12 cell suspension at 2x10 4 cells/ml in RPMI-1640/10% horse serum/5% FBS. Add 2ml of cell suspension to each well.
4. Prepare NGF as described on certificate of analysis to a final concentration of 20ng/ml. 5. Incubate plates at 37 C for 3-5 days.
6. Record neurite outgrowth at each laminin concentration.
34 Junction Square Drive
Concord MA 01742-3049
USA
phone (978) 371-6446 or (800) 370-2222
fax (978) 371-2266
EMAIL:antibodies@fitzgerald-fii.com
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