rev: February 14, 2005
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CYTOKERATIN
ANTIBODIES
(anti-Human and others as indicated)
RDI Division of Fitzgerald Industries Intl offers a wide line of antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.
RDI-PRO10526 $375.00
Clone Determination M20
Category Mouse monoclonal
Form/Purification Culture supernatant
Ig Class IgG 1
Immunogen Human breast carcinoma cell line
Description/Specificity In immunoblotting, the antibody is directed against the 52.2 kDa cytokeratin protein (cytokeratin 8). M20 is exclusively reactive with cytokeratin 8. Cytokeratin 8 is present in simple type epithelia, in glandular epithelium and in most tumors derived therefrom. Epitope has been mapped to aa 353-367 on alpha helical red domain (see Waseem et al, 2004)
Recommended positive control: Glandular epithelia
Application
Suitable for immunohistochemistry of frozen tissue and cell
suspensions
Antigen Recognized Human, mouse, rat, rabbit (most mammals)
in Species (tested so far)
Working Dilution 1:5 - 1:10, dilute immediately before use
Dilution Buffer PBS with 1% BSA and 0.1% Na-Azide
Incubation Time 1 h at room temperature
Storage At 2-8°C (undiluted) or at -20°C (aliquots)
Volume 1 ml
Reference
1. Edwards, B.F.., et al. Transitional cell cytokeratins as a second parameter in flow cytometry of bladder cancer (1987). World Journal Urology 5:123-127.
2. Muijen, G.N.P. van, et al. Coexpression of intermediate filament polypeptides in human fetal and adult tissues (1987). Lab. Invest 57: (4), 359-369.
3. Muijen, G.N.P. van, et al. Differentiation-related changes of cytokeratin expression in cultured keratinocytes and in foetal, newborn and adult epidermis (1987). Exp. Cell Research 171:331-345.
4. Waseem A et al, Conformational changes in the rod domain of human keratin 8 following heterotypic association with keratin 18 and its implication for filament stability. Biochemistry 43, 1283-1295 (2004)
For Research Use Only
RDI Division of Fitzgerald Industries Intl
34 Junction Square Drive
Concord MA 01742-3049
USA
phone (978) 371-6446 or (800) 370-2222
fax (978) 371-2266
EMAIL:antibodies@fitzgerald-fii.com
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