rev: 5/15/2008
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CYTOKERATIN ANTIBODIES
(anti-Human and others as indicated)
RDI Division of Fitzgerald Industries Intl offers a wide line of antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.
ANTI-CYTOKERATIN 5 + 8
Mab to Cytokeratin 5 and 8
cat#RDI-PRO10521, new cat#: 10R-C170a $412.50
Clone RCK 102
Category Mouse monoclonal
Form/Purification Culture supernatant
Ig Class IgG1
Immunogen Lung cancer cell preparation
Clinical Application/Specificity Mab RCK 102, directed against the 58 kDa and
52.2 kDa cytokeratin proteins (cytokeratins 5 and 8 in the Moll catalogue of
cytokeratins), reacts with all epithelial cell types. No reaction is observed
in non-epithelial tissues. The antibody can be used for the discrimination of
carcinomas from non-epithelial tumors such as lymphomas, sarcomas and neural
tumors. RCK 102 is applicable on frozen and paraffin-embedded tissue. Formalin
fixation may cause antigen denaturation or masking. Therefore, protease
digestion is necessary prior to antibody application.
Antigen Recognized in Species (tested so far) Human, most mammals including mouse, rabbit and rat
Positive Control : Skin, cervix
Suitable for · Immunofluorescence
· Immunohistochemistry
· Immunoblotting
· Applicable on frozen and paraffin sections Þ Proteolytic treatment with pronase or trypsin required
Dilution Buffer PBS with 1% BSA and 0.1% Na-Azide
Working Dilution 1:5 - 1:10
Storage At 4°C (undiluted)
Volume 1 ml
References
Broers JLV, et al: Intermediate filament proteins in classic and variant types of small cell lung carcinoma cell lines: a biochemical and immunochemical analysis using a panel of monoclonal antibodies . J Cell Science 83:37-60 (1986)
Eyken P van: Cytokeratin-immunohistochemical study of Hepatoblastoma. Human Pathology 21:302-308 (1990)
For research use only. Not supplied for use in human diagnostic or therapeutic procedures
RDI Division of Fitzgerald Industries Intl
phone (800) 370-2222 or (201) 584-7093
fax (978) 371-2266
EMAIL:antibodies@fitzgerald-fii.com
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