rev: September 13, 1999

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CYTOKERATIN   ANTIBODIES  

(anti-Human and others as indicated)

RDI Division of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.

ANTI-CYTOKERATIN  TYPE 18
Mab to Cytokeratin 18

cat#RDI-PRO10500      $344.00/vial 1 ml


Clone RGE 53


Category Mouse monoclonal


Form/Purification culture supernatant


Ig Class IgG1


Antigen Isolated from HeLa cells


Clinical Application/Specificity Mab specifically recognizes glandular epithelial cells from the gastrointestinal tract, the respiratory tract and the urogenital tract, as well as endocrine and exocrine tissues and myoepithelial cells. No reaction with stratified squamous epithelia. In the transitional epithelium of the bladder RGE 53 reacts only with superficial (umbrella) cells.The antibody is useful for the discrimination of adenocarcinomas and meso-theliomas from squamous cell carcinoma and non-epithelial tumors. Polypeptide reacting: 45 kDa cytokeratin 18


Antigen Recognized in Species (tested so far) Human, most mammals incl. mouse, rat, rabbit; birds

Suitable for · Immunofluorescence

                  · Immunohistochemistry

                  · Immunoblotting

                  · Applicable on frozen sections

Dilution Buffer PBS with 1% BSA and 0.1% Na-Azide


Working Dilution 1:5-1:10


Storage At 4°C (undiluted)


Volume 1 ml

References

Ramaekers, F et al. Monoclonal Antibody to Keratin Filaments, specific for Glandular Epithelia and their Tumors. Lab Invest 9:353-361 (1983)

Puts, J et al. Expression of Cytokeratins in Early Neoplasia Epithelial Lesions of the Uterine Cervix. Int J Gyn Path 4:300-313

Smedts, F et al. Changing Patterns of Keratin Expression During Progression of Cervical Intraepithelial Neoplasia. Am J Pathol 136:657-668 (1990)

For research use only. Not supplied for use in human diagnostic or therapeutic procedures

RDI Division of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

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