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ANTIBODIES  

(anti-Human and others as indicated)

RDI Divison of researchd Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.


Mouse anti-PMCA ATPase Monoclonal ab

cat# RDI-PMCA/5F10      $469.00/vial


Background: The Ca2+ pump of the plasma membrane, termed PMCA ATPase pumps CA2+ from the cytosol to the extracellular space. This membrane bound enzyme is related to a number of other ATPases including the SERCA ATPase and the NA K Pump. Studies indicate there are four known isoforms of the PMCA ATPase which are encoded by a multigene family.

isotype: mIgG2a

clone: 5F10

package: 100ul of diluted ascities fluid with 0.05% sodium azide added

antigen: produced from a fusion of FO myeloma cells and spleen cells from Balb/c mice immunized with purified human erythrocyte CA2+ ATPase.

Specificity: epitope mapping between amino acids 724-783 of the human erythrocyte CA2+ pump. This epitope is located in the highly conserved hinge region which is on the intracellular loop of PMCA ATPase between the putative transmembrane domains M4 and M5. This clone reacts with all four known isoforms of PMCA ATPase and a wide range of species and tissues as shown below.



Human: erythrocyte, kidney, placenta, choroid plexus

Primate: kidney

Canine: kidney

Feline: erythrocyte, choroid plexus

Chicken: oviduct, intestine

Rat: erythrocyte, liver, kidney, aortic

        endothelial, intestine, placenta,

        enamel/dentin, brain, choroid plexus.

Hamster: erythrocyte, liver, kidney, aortic

               endothelial, intestine, brain

Eel: kidney

RABBIT: kidney



Use:

-Western blot 1:1000 recognizes approx 140kDa band representing PMCA ATPase and bands at 95Kda and 180Kda which are probably products of aggregation and/or natural proteolytic products of the pump.

-Immunohistochemistry: 1:500 staining depends upon tissue being studied and location of the pump. Suitable for both frozen and paraffin embedded tissues.

-Immunoprecipitation



Storage: Store at -20 DEG C upon receipt. Avoid frequent freeze thaw cycles. Recommend aliquoting. BEFORE OPENING, suggest centrifuging for 1 minute at 1000 RPM to concentrate material in vial.


References: -J.L. Borke, A. Caride, A.K. Verma, J.T. Penniston

                    and R. Kumar. Plasma membrane calcium pump and 28

                     kDa calcium binding proteins in cells of rat

                     kidney distal tubes. American Journal of Physiology, 257:F842-F849, 1989.

                    -Journal of Biological Chemistry, 267: 14244- 14249, 1992.

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.


It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.


Also available: antibodies to NA K ATPase and CA ATPASE (sera 1 , serca 2 and serca 3). Please inquire.


RDI Divison of researchd Industries Intl

San Jose, 95123 CA Snell ave 658

USA

or (800) 370-2222

EMAIL:margaret@cellular-research.com

Ordering terms

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