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ANTIBODIES
(anti-Human and others as indicated)
RDI Divison of researchd Industries Intl offers a wide line of antibodies. Since no one antibody works best for all applications (neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.
Mouse anti-PMCA ATPase Monoclonal ab
cat# RDI-PMCA/5F10 $469.00/vial
Background: The Ca2+ pump of the plasma membrane, termed PMCA ATPase pumps
CA2+ from the cytosol to the extracellular space. This membrane bound enzyme
is related to a number of other ATPases including the SERCA ATPase and the
NA K Pump. Studies indicate there are four known isoforms of the PMCA ATPase
which are encoded by a multigene family.
isotype: mIgG2a
clone: 5F10
package: 100ul of diluted ascities fluid with 0.05% sodium azide added
antigen: produced from a fusion of FO myeloma cells and spleen cells from
Balb/c mice immunized with purified human erythrocyte CA2+ ATPase.
Specificity: epitope mapping between amino acids 724-783 of the human erythrocyte
CA2+ pump. This epitope is located in the highly conserved hinge region which
is on the intracellular loop of PMCA ATPase between the putative transmembrane
domains M4 and M5. This clone reacts with all four known isoforms of PMCA
ATPase and a wide range of species and tissues as shown below.
Human: erythrocyte, kidney, placenta, choroid plexus
Primate: kidney
Canine: kidney
Feline: erythrocyte, choroid plexus
Chicken: oviduct, intestine
Rat: erythrocyte, liver, kidney, aortic
endothelial, intestine, placenta,
enamel/dentin, brain, choroid plexus.
Hamster: erythrocyte, liver, kidney, aortic
endothelial, intestine, brain
Eel: kidney
RABBIT: kidney
Use:
-Western blot 1:1000 recognizes approx 140kDa band representing PMCA ATPase and bands at 95Kda and 180Kda which are probably products of aggregation and/or natural proteolytic products of the pump.
-Immunohistochemistry: 1:500 staining depends upon tissue being studied and location of the pump. Suitable for both frozen and paraffin embedded tissues.
-Immunoprecipitation
Storage: Store at -20 DEG C upon receipt. Avoid frequent freeze thaw cycles.
Recommend aliquoting. BEFORE OPENING, suggest centrifuging for 1 minute at
1000 RPM to concentrate material in vial.
References: -J.L. Borke, A. Caride, A.K. Verma, J.T. Penniston
and R. Kumar. Plasma membrane calcium pump and 28
kDa calcium binding proteins in cells of rat
kidney distal tubes. American Journal of Physiology, 257:F842-F849, 1989.
-Journal of Biological Chemistry, 267: 14244- 14249, 1992.
Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.
It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.
Also available: antibodies to NA K ATPase and CA ATPASE (sera 1 , serca 2
and serca 3). Please inquire.
RDI Divison of researchd Industries Intl
San Jose, 95123 CA Snell ave 658
USA
or (800) 370-2222
EMAIL:margaret@cellular-research.com
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