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ANTIBODIES  

(anti-Human and others as indicated)

RDI Divison of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.


NA CA Exchanger (see below 1 polyclonal and 2 monoclonals)
BATCH SHEET: Mouse anti-NA CA Exchanger Protein (clone ref#R3F1) monkey/horse/dog/rat/bovine/mouse

Catalog#: RDI-NACAEXCH-F1     Price: $688.00/vial

Packaging: 1 X 200 microliters lyophilized concentrated supernatant (no preservatives) approx 140 micrograms IgG

Reconstitution: with 200ul distilled water

Species: mouse IgG1
Antigen: canine cardiac sarcolemmal NA+ CA+ exchanger

recognizes two neighboring but non-overlapping sequences of the hydrophilic region of the exchanger, connecting the putatative transmembrane segments 5 and 6. The antibody was evaluated for specificity and potency by immunobloting and immunoprecipitation. It recognizes virtually only NCX1 in monkey, horse, dog, rat, bovine an dmouse tissue. At higher concentrations it reacts faintly with NCX2, whereas NCX3 is not recognized. Both splice variants with exon A (heart, nervous system) and exon B (kidney, glia) are recognized.

ses: Immunoblots: 1:1000-1:5000 (for chemiluminesece detection, recommend starting 1:5K). A strong band 70kda is found in addition to the 116kda of the integral exchanger. Of the HMW band is a double band 116/112kda band

immunoprecipitation:1K-5K

cytochemistry: 1:1000 +with avidin biotin (ABC) method

-on cultured neonatal rat myocytes, 1:1000 dilution shows strong staining of the plasmalemma.

Optimal dilutions must be determined by the end user for their particular application.

ref: Porzig H et al (1993) Mapping the cardiac sodium-calcium exchanger with monoclonal antibodies, Am. J. Physiol. 265:C78-C756, 1993.

Storage: For long term storage, keep small portions (1-2ul) at -80 DEG C or at least -20 DEG C. For continuous use keep at 4 DEG C (with 0.01% Na-azide). AVOID FREQUENT FREEZE THAW CYCLES.

Allow 2 weeks for delivery:


Rabbit anti-NA CA Exchanger Protein


Catalog#: RDI-NACAEXCH    Price: $470.00/vial    

Packaging: 200 microliters lyophilized antiserum (no preservatives)

Reconstitution: with 200ul distilled water

Species: rabbit

Antigen: canine cardiac sarcolemmal NA+ CA+ exchanger

Specificity: specific for cardiac, brain, smooth muscle and kidney NA-CA exchanger. The antiserum cross reacts with the protein from rat, rabbit, frog, toad, guinea pig, cow and human.


Uses: Immunoblots: 1:500-1:1000

         Immunohistochemistry: 1:1000- 1:2000 with avidin biotin method

Optimal dilutions must be determined by the end user for their particular application.


ref: Philipson, K.D. et al. (1988) Biochim. Biophys. Acta 945:298-306.

Storage: For long term storage, keep small portions (1-2ul) at -80 DEG C or at least -20 DEG C. For continuous use keep at 4 DEG C (with 0.01% Na-azide). AVOID FREQUENT FREEZE THAW CYCLES.

Allow 2 weeks for delivery: Also available: rabbit anti- N CA Kexchanger of rod outer segments and other ion transport antibodies. Also new Monoclonal NA-CA Exchanger cat#RDI-NACAXabm


Additional product of interest: partially purified Ca channel.

cat#RDI-CACHAN/P 250.00/10 micrograms

Order through: RESEARCH DIAGNOSTICS INC (201) 584-7093


Mouse anti-NA+ CA+ Exchanger

Catalog#: RDI-NACAXabm Price:  $469.00/vial

LOT# see sheet with shipment

Packaging: 100 ul diluted ascites in PBS with 0.05% NaN3

Species: mouse IgM

Clone: C2C12

Antigen: purified canine cardiac NA+ CA+ exchanger

Specificity: -Reacts with the Na CA exchanger in kidney and cardiac tissues of human, canine, rabbit, guinea pig and rat. Binds to epitope in the region of amino acids 371-525 (on the intracellular side of the plasma membrane).

-Has been used in western blot (not recommended for use with rat in WB), immunohistochemistry and immunoprecipitation to detect NA CA exchanger. In western blot, produces a band at 120 Kda for the mature exchanger. Additional bands at 70Kda and 160Kda represent a proteolytic fragment and non reduced exchanger respectively. -stains plasma membrane with intense staining of cardiac T-tubular membrane is seen.


Uses: western blot:1:1000

         immunofluorescence: 1:200

Optimal dilutions must be determined by the end user for their particular application.



ref:
-J.S. Frank et al, "Distribution of the NA+ CA+ exchange protein in mammalian cardiac myocytes: An Immunofluorescence and Immunocolloid Gold labeling Study. the   Journal of Cell Biology, 117 (2):337-345, 1992.

-Z. Li, et al, The Journal of Biological Chemistry, 268(16):11489-11491, 1993.


Storage: Store at -20 DEG C upon receipt. Avoid frequent freeze thaw cycles. Recommend aliquoting. BEFORE OPENING, suggest centrifuging for 1 minute at 1000 RPM to concentrate material in vial.


Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.


Also available: rabbit anti-NA CA Exchanger cat#RDI-NACAEXCH


RDI Division of Fitzgerlad Industries Intl

(Research Diagnostics Inc)

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

email: sales@researchd.com

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