rev:   January 11, 2004

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(anti-Human and others as indicated)

RDI Divison of researchd Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.

Mouse anti-Na+/K+ alpha 1 subunit Monoclonal ab (M8P1A3)

cat# RDI-atpa1M8abm     $469.00/vial

Background: The NA+/K+ Atpase is an integral membrane enzyme found in all cells of higher organisms and is responsible for the ATP-dependent transport of NA and K across the cell membrane. The enzyme consists of a large multipass transmembrane catalytic subunit (alpha subunit) and an associated smaller glycoprotein (Beta subunit). Studies indicate that there are three isoforms of the alpha subunit (A1, a2, & A3) and two isoforms of the Beta subunit (B1 & B2) which are encoded by two multigene families. The alpha 3 subunit has been found in neuronal and to a lesser extent skeletal and smooth muscle.

isotype: mIgG1

clone: M8-P1-A3

package: 200ul of diluted ascites fluid with 0.05% NaN3

antigen: produced from a fusion of Ag14 myeloma cells and spleen   cells from F1 hybrid mice immunized with purified lamb kidney alpha 1 subunit Na+ K+ Atpase.

Specificity: alpha 1 subunit of NA+/K+ ATPase

                 -in sheep, canine , rat, pig. (also sees

                   pig gastric H+/K+ ATPase)

                 -binds to region between amino acids 496 and 506 of the

                   lamb kidney NA K Atpase alpha subunit.

Use: -western blot (detects a band at approx 110kDa) 1:250


        -Immunohistochemistry: 1:100

ref: -W.J. Ball, et al, Biochimica et Biophysica Acta, 719:413-423, 1982.

Storage: Store at -20 DEG C upon receipt. Avoid frequent  freeze thaw cycles. Recommend aliquoting. BEFORE   OPENING, suggest centrifuging for 1 minute at 1000   RPM to concentrate material in vial.

Precautions: For In vitro research Use Only. Not for use in or on  humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of   this product.

RDI Divison of researchd Industries Intl

San Jose, 95123 CA Snell ave 658


or 408-780-0908

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