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ANTIBODIES
(anti-Human and others as indicated)
RDI Division of Fitzgerald Industries Intl offers a wide line of antibodies. Since no one antibody works best for all applications (neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.
Mouse anti-H+/K+ ATPase
(beta subunit) Monoclonal ab
cat# RDI-HKATPabm, new cat#: 10R-H100b $517.00/vial
Background: The H+/K+ ATPase, or gastric proton pump belongs to a family of P-
type cation-transporting adenosine 5'-triphosphatases (ATPases). This family of
ATPases shares a number of functional and structural similarities including the
common features of consisting of an A and B subunit. Like the ubiquitous NA/+K+
ATPase, the H+/K+ ATPase consists of a large transmembrane catalytic subunit,
termed the A subunit which contains sites for ATP binding and phosphorylation,
and an associated smaller glycoprotein, termed the B-subunit, which may play a
role in maintaining the structural and functional integrity of the complex.
isotype: mIgG1
clone: 2G11
package: 100ul of diluted ascites fluid in PBS with
0.05% NaN3
antigen: produced from a fusion of P3X63 AG8.653 myeloma cells and spleen cells
from Balb/c mice immunized with 34kDa core peptide purified from deglycosylated
hog gastic microsomes.
Specificity: Detects the B-subunit of the H+/K+ ATPase (a glycoprotein with a
34kDa core peptide that has a single transmembrane segment, a small cytoplasmic
N-terminal, and a large extracellular C-terminal domain with seven potential
N-linked glycosylation sites. This ab also has been shown to bind an epitope
within amino acids 1-13 or 15-28 located on the cytoplasmic side of the
B-subunit. It also inhibits the enzymatic activity of the H+/K+ ATPase and
alters the affinity of the cytoplasmic K+ binding site.
Use:
-Western blot:1:4000= recognizes
approx 60 to 80 Kda glycosylated protein, a 52kDa b-subunit precursor and the
34 kDa core peptide. Reacts with several mammalian species
including cow, dog, pig, rabbit, mouse, feret and rat.
-Immunohistochemistry: 1:2000 =
-immunoprecipitation
REF: -Dar-Chone Chow & John G. Forte, Characterization of the subunit
of the H+/K+ ATPase using an inhibitory monoclonal antibody. Am.
J. of Physiol. 265 (Cell Physiol. 34): C1562-1570, 1993.
Storage: Store at -20 DEG C upon receipt. Avoid frequent freeze thaw cycles.
Recommend aliquoting. BEFORE OPENING, suggest centrifuging for 1 minute at 1000
RPM to concentrate material in vial.
Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.
Mouse anti-H+/K+ ATPase (ALPHA subunit) Monoclonal ab
cat# RDI-HKATPAabm, new cat#: 10R-H099a $525.00/vial 100ug
Background: The H+/K+ ATPase, or gastric proton pump belongs to a family of
P- type cation-transporting adenosine 5'-triphosphatases (ATPases). This family
of ATPases shares a number of functional and structural similarities including
the common features of consisting of an A and B subunit. Like the ubiquitous
NA/+K+ ATPase, the H+/K+ ATPase consists of a large transmembrane catalytic
subunit, termed the A subunit which contains sites for ATP binding and
phosphorylation, and an associated smaller glycoprotein, termed the B-subunit,
which may play a role in maintaining the structural and functional integrity of
the complex.
isotype: mIgG1
clone: 1H9
package: 100ug (lyophilized) or 100ug (in 0.1ml PBs with 50% glycerol)
antigen: myeloma (SP2/0-Ag14) X Spleen cells of Balb/c nu/+ mouse with
neonatal thymectomy-induced autoimmune gastritis
Specificity: Detects the Alpha-subunit of the H+/K+ ATPase from mouse, human,
bovine and rat
Use: -Western blot:2-10ug/ml
-Immunohistochemistry: 2-10ug/ml
-immunoprecipitation:
REF: -Mori, Y et al:Gastroenterology, 97:364-375,1989
-Jones, C.M. et al:Gastroenterology, 101:287-294, 1991
-Toh,B et al:Proc
Natl Acad Sci
-Nishio, A et al:Gastroenterology,
107:1408-1414,1994
Storage: Store at 4 DEG C or below upon receipt. After reconstitution,
recommend aliquoting and store at -20 DEg C.
Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.
Mouse anti-H+/K+ ATPase (BETA subunit) Monoclonal ab
cat# RDI-HKATPB6abm, new cat#: 10R-H100a $525.00/vial 100ug
Background: The H+/K+ ATPase, or gastric proton pump belongs to a family of P- type cation-transporting adenosine 5'-triphosphatases (ATPases). This family of ATPases shares a number of functional and structural similarities including the common features of consisting of an A and B subunit. Like the ubiquitous NA/+K+ ATPase, the H+/K+ ATPase consists of a large transmembrane catalytic subunit, termed the A subunit which contains sites for ATP binding and phosphorylation, and an associated smaller glycoprotein, termed the B-subunit, which may play a role in maintaining the structural and functional integrity of the complex.
isotype: mIgG1
clone: 2B6
package: 100ug (in 0.1ml PBs with 50% glycerol)
antigen: myeloma (SP2/0-Ag14) X Spleen cells of Balb/c nu/+ mouse with neonatal thymectomy-induced autoimmune gastritis
Specificity: Detects the Beta-subunit of the H+/K+ ATPase from mouse, human,
bovine and rat
Use: -Western blot:2-10ug/ml
-Immunohistochemistry: 2-10ug/ml (high temp antigen release 2 X 10
minutes in citrate buffer pH6.5)
-immunoprecipitation: 5-10ug/200-300ul cell extract
REF: -Mori, Y et al:Gastroenterology, 97:364-375,1989
-Jones, C.M. et al:Gastroenterology, 101:287-294, 1991
-Toh,B et al:Proc Natl
Acad Sci
-Nishio, A et al:Gastroenterology, 107:1408-1414,1994
Storage: Store at 4 DEG C or below upon receipt. After reconstitution, recommend aliquoting and store at -20 DEg C.
Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.
RDI Division of Fitzgerald Industries Intl
phone (978) 371-6446 or (800) 370-2222
fax (978) 371-2266
EMAIL:antibodies@fitzgerald-fii.com
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