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ANTIBODIES  

(anti-Human and others as indicated)

RDI Divison of researchd Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.


Mouse anti-H+/K+ ATPase (beta subunit) Monoclonal ab

cat# RDI-HKATPabm    $469.00/vial

Background: The H+/K+ ATPase, or gastric proton pump belongs to a family of P- type cation-transporting adenosine 5'-triphosphatases (ATPases). This family of ATPases shares a number of functional and structural similarities including the common features of consisting of an A and B subunit. Like the ubiquitous NA/+K+ ATPase, the H+/K+ ATPase consists of a large transmembrane catalytic subunit, termed the A subunit which contains sites for ATP binding and phosphorylation, and an associated smaller glycoprotein, termed the B-subunit, which may play a role in maintaining the structural and functional integrity of the complex.

isotype: mIgG1

clone: 2G11

package: 100ul of diluted ascites fluid in PBS with 0.05% NaN3

antigen: produced from a fusion of P3X63 AG8.653 myeloma cells and spleen cells from Balb/c mice immunized with 34kDa core peptide purified from deglycosylated hog gastic microsomes.

Specificity: Detects the B-subunit of the H+/K+ ATPase (a glycoprotein with a 34kDa core peptide that has a single transmembrane segment, a small cytoplasmic N-terminal, and a large extracellular C-terminal domain with seven potential N-linked glycosylation sites. This ab also has been shown to bind an epitope within amino acids 1-13 or 15-28 located on the cytoplasmic side of the B-subunit. It also inhibits the enzymatic activity of the H+/K+ ATPase and alters the affinity of the cytoplasmic K+ binding site.

Use:
     -Western blot:1:4000= recognizes approx 60 to 80 Kda glycosylated protein, a 52kDa b-subunit precursor and the 34 kDa core peptide. Reacts with several mammalian species including cow, dog, pig, rabbit, mouse, feret and rat.

   -Immunohistochemistry: 1:2000 =

   -immunoprecipitation


REF: -Dar-Chone Chow & John G. Forte, Characterization of the subunit  of the H+/K+ ATPase using an inhibitory monoclonal antibody. Am.   J. of Physiol. 265 (Cell Physiol. 34): C1562-1570, 1993.

Storage: Store at -20 DEG C upon receipt. Avoid frequent freeze thaw cycles. Recommend aliquoting. BEFORE OPENING, suggest centrifuging for 1 minute at 1000 RPM to concentrate material in vial.

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.


Mouse anti-H+/K+ ATPase (ALPHA subunit) Monoclonal ab

cat# RDI-HKATPAabm     $500.00/vial 100ug

Background: The H+/K+ ATPase, or gastric proton pump belongs to a family of P- type cation-transporting adenosine 5'-triphosphatases (ATPases). This family of ATPases shares a number of functional and structural similarities including the common features of consisting of an A and B subunit. Like the ubiquitous NA/+K+ ATPase, the H+/K+ ATPase consists of a large transmembrane catalytic subunit, termed the A subunit which contains sites for ATP binding and phosphorylation, and an associated smaller glycoprotein, termed the B-subunit, which may play a role in maintaining the structural and functional integrity of the complex.

isotype: mIgG1

clone: 1H9

package: 100ug (lyophilized) or 100ug (in 0.1ml PBs with 50% glycerol)

antigen: myeloma (SP2/0-Ag14) X Spleen cells of Balb/c nu/+ mouse with neonatal thymectomy-induced autoimmune gastritis

Specificity: Detects the Alpha-subunit of the H+/K+ ATPase from mouse, human, bovine and rat

Use: -Western blot:2-10ug/ml

         -Immunohistochemistry: 2-10ug/ml

         -immunoprecipitation:

REF: -Mori, Y et al:Gastroenterology, 97:364-375,1989

         -Jones, C.M. et al:Gastroenterology, 101:287-294, 1991

         -Toh,B et al:Proc Natl Acad Sci USA 87:6418-6422,1990

         -Nishio, A et al:Gastroenterology, 107:1408-1414,1994

Storage: Store at 4 DEG C or below upon receipt. After reconstitution, recommend aliquoting and store at -20 DEg C.

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.


Mouse anti-H+/K+ ATPase (BETA subunit) Monoclonal ab

cat# RDI-HKATPB6abm     $500.00/vial 100ug

Background: The H+/K+ ATPase, or gastric proton pump belongs to a family of P- type cation-transporting adenosine 5'-triphosphatases (ATPases). This family of ATPases shares a number of functional and structural similarities including the common features of consisting of an A and B subunit. Like the ubiquitous NA/+K+ ATPase, the H+/K+ ATPase consists of a large transmembrane catalytic subunit, termed the A subunit which contains sites for ATP binding and phosphorylation, and an associated smaller glycoprotein, termed the B-subunit, which may play a role in maintaining the structural and functional integrity of the complex.

isotype: mIgG1

clone: 2B6

package: 100ug (in 0.1ml PBs with 50% glycerol)

antigen: myeloma (SP2/0-Ag14) X Spleen cells of Balb/c nu/+ mouse with neonatal thymectomy-induced autoimmune gastritis

Specificity: Detects the Beta-subunit of the H+/K+ ATPase from mouse, human, bovine and rat

Use: -Western blot:2-10ug/ml

         -Immunohistochemistry: 2-10ug/ml (high temp antigen release 2 X 10

          minutes in citrate buffer pH6.5)

-immunoprecipitation: 5-10ug/200-300ul cell extract

REF: -Mori, Y et al:Gastroenterology, 97:364-375,1989

        -Jones, C.M. et al:Gastroenterology, 101:287-294, 1991

        -Toh,B et al:Proc Natl Acad Sci USA 87:6418-6422,1990

       -Nishio, A et al:Gastroenterology, 107:1408-1414,1994

Storage: Store at 4 DEG C or below upon receipt. After reconstitution, recommend aliquoting and store at -20 DEg C.

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.


RDI Divison of researchd Industries Intl

San Jose, 95123 CA Snell ave 658

USA

or (800) 370-2222

EMAIL:margaret@cellular-research.com

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