rev: Febraury 10, 2005
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CYTOKERATIN &
RELATED
ANTIBODIES
(anti-Human and others as indicated)
RDI Divison of Fitzgerald Industries Intl offers a wide line of antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.
ANTI-FIBRILLIN
Mouse anti-Fibrillin-1 clone 12A5.18 Human/Bovine
(Paraffin Section/WB/Histochemsitry)
cat# RDI-FIBRILLabm $438.00/100ug
RDI-FIBRILLabm-1 $688.00/200ug
Presentation: Protein G purified antibody in 10mM PBS with 0.2% BSA and 15mM
NaN3. (Bulk available without BSA/Azide upon request) concentration
200ug/ml
Reactivity: human and bovine
Clone: 12A5.18
Isotype: mIgG1k
Immunogen: microfibrils from zonular appartus of bovine eye
Uses: -Western blot: 1-2ug/ml
-histochemistry (frozen and paraffin sections) 2-4ug/ml 60 min RT
Note:no special pretreatment is required for formalin/paraffin sections(do not heat sections in the citrate buffer)
-immunolocalization by Electron Microscopy
-immunofluorescence 5-10ug/ml
Positive Ctrl: Normal skin fibroblasts
Localization: extracellular microfibrils
Background: In western blot, this Mab recognizes a single protein of 350kDa
which is identified as fibrillin-1. It shows no cross-reaction with fibrillin-2.
Reactivity with deletion mutants indicates that the epitope spans between
aa 451-909 of fibillin-2. In immunohistochemical staining of
paraformaldehyde-fixed human skin, this antibody decorates the elastic framework
and more specifically the oxytalantic network close to the epidermis. Cultures
of normal human skin fibroblasts show discrete staining of extracellular
fibrils. No corss observed with fibrillin-2.There is an aberrant expression
of fibrillin in Marfan Synsdrome
Fibrillin-1 is an extracellular acidic protein with a high cysteine content
and an extended thread-like shape with mosaic composition of different types
of extracellular modules. Most of the fibrillin molecule is contributed by
47 epidermal growth factor-like (EGF-like) repeats; 43 of them have a consensus
sequence for calcium binding (cb). Other modules including 8-cysteine motifs
(seven), hybrid motifs (two), and unique domains (three) are interspersed
throughout the molecule[3-7]. fibrillin-1 contains a proline-rich region
close to the N-terminus that is replaced by a glycine-rich region in fibrillin-2.
The fibrillins are found throughout the connective tissue as integral components
of extended fibrils.
Storage: 2-8 deg c
ref: 1) Maier, A et al, Fibrillin and elastin networks in extrafusal tissue
and muscle spindles of bovie extraocular muscles. Investigative Opthalmology
and Visual Science, 1994, 35(7):3103-10
2) McDaniels CN, et al, Immunization with undenatured
bovine zonular fibrils results in monoclonal antibodies to fibrillin. Matrix
Biology, 1994,14(1):41-9
3) Wright DW et al, Matrix Biology, 1994, 14(1):41-9
For Research Use Only
see also clone 11C1.3, cat#RDI-FIBRILLabm-C $438.00/100ug $688.00/200ug
(not recommended for paraffin sections) good for WB, immunofluorescence, mIgG1k isotype, see above references for details.
For research use only. Not supplied for use in human diagnostic or therapeutic procedures
34 Junction Square Drive
Concord MA 01742-3049
USA
phone (978) 371-6446 or (800) 370-2222
fax (978) 371-2266
EMAIL:antibodies@fitzgerald-fii.com
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