rev:    March 17, 2005

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ESTROGEN RECEPTOR   ANTIBODIES  

(anti-Human and others as indicated)

RDI Divison of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.

-also available recombinant human Estrogen receptor alpha cat#RDI-ERALPHA-AG   $594.00/50ug

cat#RDI-ERECPCabrX       $438.00/0.5ml

cat#RDI-ERECPCabrX-1   $688.00/1.0ml

-Protein A purified antibody (1mg/ml with 0.5% BSA and 0.09% sodium  azide)

-immunogen: synthetic peptide derived from C terminus of human estrogen alpha

-reactivity: human, mouse, and hmaster (not rat ,others not tested)

-use: paraffin sections (with citrate buffer release), westernblot, immunoprecipitation

-histochemistry blocking peptide cat#RDI-ERECPCabrX-CP $188.00/200ug in 1ml with BSA and azide

cat#RDI-ERECPNabrX      $438.00/0.5ml

cat#RDI-ERECPNabrX-1   $688.00/1.0ml

-Protein A purified antibody (1mg/ml with 0.5% BSA and 0.09% sodium  azide)

-immunogen: synthetic peptide derived from N terminus of human estrogen alpha

-reactivity: Human, Rat, Mouse, Rabbit, Sheep, Pig, and Cow. Others-not known.

-use: paraffin sections (with citrate buffer release, C term antibody is preferred ), westernblot, immunoprecipitation

-histochemistry blocking peptide cat#RDI-ERECPNabrX-CP $188.00/200ug in 1ml with BSA and azide

cat#RDI-ERED56F-COC    $312.00/0.1ml

cat#RDI-ERED56F-COC1   $688.00/0.5ml

-cocktail of clones 1D5 & 6F11 (supernatant)

-reactivity: human and rat (others not tested)

-not epitope mapped

-use: acetone fixed forzen and formalin fixed or alcohol fixed paraffin sections or western blot

cat#RDI-ERAER1D5      $625.00/100ug mIgG1

#RDI-ERAER1D5-1       $1125.00/200ug 200ug/ml in PBS with BSA & NaN3

#RDI-ERAER1D5-1X     $938.00/100ug (1mg/ml in PBS, no azide/no BSA)

#RDI-ERAER1D5-2X     $1188.00/200ug (1mg/ml in PBS, no azide/no BSA)

-clone 1D5

-mouse IgG1

-binds in N terminal domain

-human, mouse, rat and monkey reactive

-suitable for western blot(67kDa), and histochemisry on formalin fixed paraffin sections

-antigen: rec human estrogen receptor

cat#RDI-ERAER611          $562.00/0.5ml mIgG1

      #RDI-ERAER611-1      $938.00/1ml

supernatant , mIgG1

-suitable for western blot(67kDa), immunoprecipitation (native or denatured)

-not epitope mapped

-human, mouse ,rat and hamster reactive (others not tested)

-antigen:rec full length estrogen receptor

cat#RDI-ERAER308        $406.00/100ug mIgG1

      #RDI-ERAER308-1   $625.00/200ug

-10mM PBS with 0.2% BSA and 15mM NaN3 (200ug/ml) Bulk, NO BSA on request

-suitable for western blot(67kDa), immunoprecipitation, frozen and paraffin sections, and supershift assays

-binds to region aa280-335 of D/E domain of ER, a hsp90 binding site (reacts with hinge region and interferes with the DNA binding function of the receptor)

-human, mouse ,rabbit and bovine reactive

-antigen:purified, SDS denatured calf uterus ER

-recognizes a protein of 67kDa identified as ER, upon chymotyrptic cleavage of ER, this antibody recognizes a 34kDa fragment. The epitope recognized by this antibody is better accessible in the denatured ER.

cat#RDI-ERECPD11X      $406.00/100ug mIgG1

#RDI-ERECPD11X-1      $625.00/200ug

-10mM PBS with 0.2% BSA and 15mM NaN3 (200ug/ml) Bulk, NO BSA on request

-binds to region aa302-553 of E/F domain of ER)

-human , rat, mouse, bovine, pig, rabbit, sheep, hamster and chicken reactive

-antigen:35kDa, C terminal fragment of human estrogen receptor which was expressed in E.Coli as a GST fusion protein

-recognizes a protein of 67kDa identified as ER

-western blot (approx 1-2ug/ml)

-blocks binding of estrogen receptor to ER (to order without azide or BSA, cat#RDI-ERECPD11XP $688.00/200ug)

-immunoprecipitation (native or denatured)

cat#RDI-ERAER314     $406.00/100ug mIgG1

     #RDI-ERAER314-1    $625.00/200ug

-10mM PBS with 0.2% BSA and 15mM NaN3 (200ug/ml) Bulk, NO BSA on request

-suitable for gel supershift, western blot(67kDa) and  immunoprecipitation, frozen and paraffin sections (for paraffin recomend clone TE511.5D111, ascites/tcs form)

-binds to region aa120-170 (I group) of B domain of human ER)

-human, mouse, rat, rabbit and bovine reactive

-antigen:purified, SDS denatured calf uterus ER

-On chymotryptic cleavage of ER, clone AER314 recognized only the undigested ER indicating that its epitope is dismembered by digestion.

cat#RDI-ERAER304      $406.00/100ug

     #RDI-ERAER304-1  $625.00/200ug

-10mM PBS with 0.2% BSA and 15mM NaN3 (200ug/ml) Bulk, NO BSA on request

-suitable for gel supershift, western blot(67kDa), immunoprecipitation

-binds to region aa120-170 (I group) of B domain of human ER)

-human, mouse, bovine and chicken reactive

-antigen:purified, SDS denatured calf uterus ER (mIgG1 isotype)

-On chymotryptic cleavage of ER, clone AER304 recognized only the undigested ER indicating that its epitope is dismembered by digestion.

cat#RDI-ERAER315       $406.00/100ug mIgG1

     #RDI-ERAER315-1   $625.00/200ug

-10mM PBS with 0.2% BSA and 15mM NaN3 (200ug/ml) Bulk, NO BSA on request

-suitable for gel supershift, western blot(67kDa), immunoprecipitation

-binds to region aa340-500 (III group) of E domain of human ER)

-human (recombinant only) and bovine and chicken reactive

-antigen:purified, SDS denatured calf uterus ER

-On chymotryptic cleavage of ER, clone AER304 recognized a fragment of 34kDa. Clone AER315 antibody is unable to interact with the preformed ER- DNA complexes. DNA displaces the antibody in the preformed receptor- antibody complexes indicating that the receptor-DNA binding is stronger than the receptor-cloneAER315 Mab binding.

cat#RDI-ERAER303      $406.00/100ug mIgG2a

    #RDI-ERAER303-1   $625.00/200ug

-10mM PBS with 0.2% BSA and 15mM NaN3 (200ug/ml) Bulk, NO BSA on request

-suitable for gel supershift, western blot(67kDa), immunoprecipitation

-binds to region aa495-595 (IV group) of E/F domain of human ER)

--human, bovine reactive (not mouse)

-antigen:purified, SDS denatured calf uterus ER

-On chymotryptic cleavage of ER, clone AER303 recognized a fragment of 26kDa. the electrophoretic mobility of ER-DNA complex is partially retarded by this antibody

cat#RDI-ERAER311     $406.00/100ug mIgG2a

    #RDI-ERAER311-1   $625.00/200ug

-10mM PBS with 0.2% BSA and 15mM NaN3 (200ug/ml) Bulk, NO BSA on request

-suitable for gel supershift, western blot(67kDa), immunoprecipitation and histochemistry (frozen and paraffin sections with high temp release)

-binds to region aa495-595 (group V) of E/F domain of human ER)

-human, mouse, bovine, rat and pig reactive

-antigen:SDS denatured calf uterus ER

-On chymotryptic cleavage of ER, clone AER311 recognized undigested AND a fragment of 36kDa. Receptor DNA complex formed in the presence of clone AER311 Mab was reportedly larger than the complex formed with other antibodies, suggesting that clone AER311 Mab could concurrently bind both epitopes present in the homodimer of ER.

cat#RDI-ERAER317         $406.00/100ug mIgG2a

     #RDI-ERAER317-1      $625.00/200ug

-10mM PBS with 0.2% BSA and 15mM NaN3 (200ug/ml) Bulk, NO BSA on request

-suitable for affinity purification (in Bulk form) gel supershift,

western blot (67kDa), immunoprecipitation

-binds to region aa495-595 (IV group) of E/F domain of human ER)

-human, mouse and bovine reactive

-antigen:purified, SDS denatured calf uterus ER

-On chymotryptic cleavage of ER, clone AER317 recognized a fragment of 36kDa. The electrophoretic mobility of ER-DNA complex is partially retarded by this antibody.

cat#RDI-ERD75abrt     $469.00/100ug ratIgG2a

#RDI-ERD75abrt-1       $750.00/200ug

-10mM PBS with 0.2% BSA and 15mM NaN3 (200ug/ml) Bulk, NO BSA on request

-suitable for histochemistry (paraffin), western blot (67kDa),

-human, baboon, chimpanzee, monkey reactive

-antigen:estrogen receptor from MCF-7 cells

cat#RDI-ERH222abrt      $469.00/100ug ratIgG1

#RDI-ERH222abrt-1       $750.00/200ug

-10mM PBS with 0.2% BSA and 15mM NaN3 (200ug/ml) Bulk, NO BSA on request

-suitable for histochemistry(paraffin-recommend clone H226), western blot (67kDa),

-human (broad species)

-antigen:estrogen receptor from MCF-7 cells

cat#RDI-ERH226abrt      $469.00/100ug ratIgG1a

#RDI-ERH226abrt-1       $750.00/200ug

-10mM PBS with 0.2% BSA and 15mM NaN3 (200ug/ml) Bulk, NO BSA on request -suitable for histochemistry(paraffin), western blot (67kDa),

-human (broad species)

-antigen:estrogen receptor from MCF-7 cells

-epitope: A/B region(transactivating domain)

The hybridoma from which this antibody was produced was the product of a fusion between Sp2/0 myeloma cells and splenocytes from a BALB/c mouse immunized with a synthetic peptide whose sequence was derived from the ER-DNA binding, amino acids 247-261. The DNA-binding domain of the ER spans amino acids 185-261.

-sequence E247 V G M M K G G I R K D R R G261

Isotype: mIgG1

Specificity: This clone reacts with the estrogen receptor from human, calf, rat and mouse tissues and does not cross with the progesterone receptor or glucocorticoid receptor.

-reacts with both the steroid occupied and unoccupied forms of ER and does not bind to native, steroid binding from of ER. Some studies have shown that this clone does not inhibit binding of the ER to DNA and in fact the antibody is released from the ER upon DNA binding of the receptor.

USE: immunolocalization (5ug/ml immunoprecipitation (must titer)

Optimal dilutions must be obtained for all applications.

Storage: -20 C. Avoid repeated freeze/thaw cycles.

Reference : A. Traish et al, Steroids,55:196-208, 1990.

For IN Vitro Research USe Only-Not for use in diagnostics

cat#RDI-ERECPBCabrX         $438.00/0.5ml

cat#RDI-ERECPBCabrX-1      $750.00/1.0ml

cat#RDI-ERECPBCabrXP     $812.00/1ml no azide/no Bsa formulation

-Protein A purified antibody (1mg/ml with 0.5% BSA and 0.09% sodium azide)

-immunogen: synthetic peptide derived from C terminus of human estrogen receptor Beta

-reactivity: human NOT RAT (others not tested)

-use: , westernblot

-histochemistry blocking peptide cat#RDI-ERECPBCabrX-CP $188.00/200ug in 1ml with BSA and azide

cat# RDI-ERBETAHabr     $469.00/vial

Package: 50 ug in 0.050ml PBS of epitope affinity-purified rabbit IgG with 1.0 mg/ml BSA & 0.05% sodium azide

Immunogen: produced by immunizing New Zealand white rabbits with a synthetic peptide corresponding to the N- terminal amino acid residues 55-70 of rat and mouse ERB conjugated to KLH. This sequence is completely conserved in human and marmoset ERB protein.

Peptide Sequence. A55 EPQKSPWCEARSLEH70

Background: Steroid receptors are ligand-dependent intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate hormone. A receptor for estrogen (ER) was first cloned in 1987 and has been shown to be expressed and transcriptionally active in a number of tissues including ovary, testis, prostate and brain. Until recentlty, only the "classical" ER (ERa) has been known. However, a new estrogen binding receptor,referred to as ERB, has recently been cloned in rat and humans.

    ERB has been shown to be a high affinity estrogen binding protein capable of initiating transcription of genes under the control of estrogen response elements (ERE;s). RNA distribution studies have shown ERB is transcribed in human thymus, spleen, ovary and testis as well as rat ovary and prostate. This distribution overlaps with but is not identical to the tissue distribution of ERa RNA. While ERB RNA distribution has been studied, little is known about protein distribution under various hormone conditions. ERB protein distribution may differ from RNA distribution and from ERa protein given the complex and differing activation, dimerization and translocation pathways exhibited by various steroid receptors under various ligand states.

Reactivity: Reacts with various cell types in normal and ERa knock- out mice and normal rats. No immunostaining is observed with pre-immune serum and specific staining is eliminated when the antibody is pre-incubated with the immunizing peptide. No immuno-cross-reactivity has been observed with ERa expressed in baculovirus.

Uses: -immunohistochemistry and gel super-shift procedures to detect ERB. Produces cytoplasmic staining in Purkinje neurons of rat cerebellum, cells in the murine pituitary gland, and cynomolgus monkey cortical amygdala. In addition ERB immunostaining has been observed in mouse Leydig cells and in the transitional epithelium of the urinary bladder in male and female wild-type and ERa knock-out mice. This antibody efficiently supershifts rat ERB/ERE (vitellogenin xenopus A2 gene). This antibody does not detect ERB protein in Western blotting applications.

Dilution: Various assay conditions require that the optimal wroking concentrations be determined by serial dilution of this product. Suggested starting dilution is:

Immunohistochemistry. 1-2 ug/ml

Gel Supershift: 1-5 ul

Storage: Store at -20'C. Avoid repeated freeze/thaw cycles.

General References:

G.G.J.M.Kuiper, et.al.: Cloning of a novel estrogen receptor expressed in rat prostate and ovary Proc.Natl. Acad.Sci. USA, 93: 5925-5930, 1996.

S. Mosselman, et.al. ERB: identification and characterization of a novel human estrogen receptor FEBS Letters, 392: 49-53, 1996.

For Research Use Only.

Also available Biotin labeled:cat#RDI-ERBETAHabr-BT:$562.00

50 ug of purified, biotinylated IgG lyopholized in phosphate-buffered saline, pH 7.2. Reconstitute with 0. ml of phosphate-buffered saline, pH 7.2. Be careful to reconstitute the entire contents of the vial, some parts of the lyophilized pellet may have become dislodged and may not be at the bottom of the vial. Dilute further as desired. Contains 0.05% Sodium Azide. Store at -20'C. Avoid repeated freeze/thaw cycles.

Also available:purified recombinant human ER Beta cat#RDI-ERBETAH-AG $406.00/25ug

                        neutralizing peptide: cat#RDI-ERBETAH-CP $188.00/50ug

-produced in Baculovirus, ER-alpha is a 66kDa hormone inducible transcription factor that can act postively or negatively in regulating genes involved in tissue growth and differentiation. This material is both soluble functionally active because the baculovirus expression system used for its expression produces a recombinant proein with post-translational modifications similar to those found in mammalian cells. This material is supplied in purifed form

and tested for both 3H Estradiol and fluorescent estrogen binding. Bulk quotes on request.

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

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