rev: November 28, 2001
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(anti-Human and others as indicated)
RDI Divison of Fitzgerald Industries Intl offers a wide line of antibodies. Since no one antibody works best for all applications (neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.
Antigen: isolated from human leiomyoma
presentation: 1ml tissue culture supernatant
Reactivity: human, most mammals including mouse, rat and rabbits
Application: Clone D9 specifically reacts with the cytoplasmic 53Kda desmin
protein. Desmin is the main intermediate filament in mature skeletal, cardiac
and smooth mucle cells. the antibody can be used to distinguish rhabdomyosarcomas
and leiomyosarcomas from ohter soft tissue tumors, lymphomas and carcinomas.
Clone D9 does not cross react with other intermediate filament proteins and
is useful for the identification of myodifferentiation in tumors.
Positive control:skeletal and smooth muscle myosarcomas
USE: histochemistry, paraffin sections (proteolytic treatment with pronase
or trypsin required), western blot
Dilution: 1:10 histochemistry (optimize for each method)
dilution buffer: PBS with 1% BSA and 0.01% Na-Azide
Storage: 4 DEG C (undiluted)
Ref: Muyen, GNP Van et al, Coexpression of Intermediate Filament Polypeptides in Fetal and Adult Tissues. Lab Invest 57 (4):359-369 (1987)
For In Votro Research Use Only
see also pure
desmin and natural chicken desmin for use as standards, controls.
Category Rabbit polyclonal
Form/Purification Rabbit serum
Ig Class IgG
Immunogen Chicken gizzard
Clinical Application/Specificity: Desmin is the main intermediate filament in mature skeletal, cardiac and smooth-muscle cells. The antibody can be used to distinguish rhabdomyo-sarcomas and leiomyosarcomas from other soft tissue tumors, lymphomas and carcinomas. It reacts with the 53 kDa desmin protein and does not crossreact with other intermediate filament proteins.
Recommended Positive Control: Skeletal and smooth muscle myosarcomas
Application · Immunoblotting
Suitable for frozen and paraffin-embedded* tissue *no proteolytic treatment
Antigen Recognized(in species tested so far): Human, most mammals,
Working Dilution 1:50 - 1:100
Dilution Buffer PBS with 1% BSA and 0.1% Na-Azide
Incubation Time 1 h at room temperature
Storage At 4°C (undiluted) or at -20°C (aliquots)
Volume 0.25 ml
Garcia-Martinez V, et al. Cell shape and cytoskeletal organisation of endothelial
cells of the semilunar heart valves in the developing chick (1986). Anat
Embryol, 174, 83 - 89.
For In Vitro Research Use Only
RDI Divison of Fitzgerald Industries Intl
34 Junction Square Drive
Concord MA 01742-3049
phone (978) 371-6446 or (800) 370-2222
fax (978) 371-2266