rev:    November 28, 2001

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ANTIBODIES  

(anti-Human and others as indicated)

RDI Divison of researchd Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.

cat#RDI-PRO10519   $375.00./vial

clone: D9

Antigen: isolated from human leiomyoma

presentation: 1ml tissue culture supernatant

Reactivity:  human, most mammals including mouse, rat and rabbits

Application: Clone D9 specifically reacts with the cytoplasmic 53Kda desmin protein. Desmin is the main intermediate filament in mature skeletal, cardiac and smooth mucle cells. the antibody can be used to distinguish rhabdomyosarcomas and leiomyosarcomas from ohter soft tissue tumors, lymphomas and carcinomas. Clone D9 does not cross react with other intermediate filament proteins and is useful for the identification of myodifferentiation in tumors.

Positive control:skeletal and smooth muscle myosarcomas

USE: histochemistry, paraffin sections (proteolytic treatment with pronase or trypsin required), western blot


Dilution: 1:10 histochemistry (optimize for each method)

dilution buffer: PBS with 1% BSA and 0.01% Na-Azide

Storage: 4 DEG C (undiluted)

Ref: Muyen, GNP Van et al, Coexpression of Intermediate Filament Polypeptides in Fetal and Adult Tissues. Lab Invest 57 (4):359-369 (1987)

For In Votro Research Use Only

see also pure recombinant human desmin and natural chicken desmin for use as standards, controls.

cat#RDI-PRO10570   $344.00/vial  

Category Rabbit polyclonal

Form/Purification Rabbit serum

Ig Class IgG

Immunogen Chicken gizzard

Clinical Application/Specificity: Desmin is the main intermediate filament in mature skeletal, cardiac and smooth-muscle cells. The antibody can be used to distinguish rhabdomyo-sarcomas and leiomyosarcomas from other soft tissue tumors, lymphomas and carcinomas. It reacts with the 53 kDa desmin protein and does not crossreact with other intermediate filament proteins.

Recommended Positive Control: Skeletal and smooth muscle myosarcomas

Application · Immunoblotting

                  · Immunohistochemistry

                  · Suitable for frozen and paraffin-embedded* tissue *no proteolytic treatment required

Antigen Recognized(in species tested so far): Human, most mammals, birds

Working Dilution 1:50 - 1:100

Dilution Buffer PBS with 1% BSA and 0.1% Na-Azide

Incubation Time 1 h at room temperature

Storage At 4°C (undiluted) or at -20°C (aliquots)

Volume 0.25 ml

References

Garcia-Martinez V, et al. Cell shape and cytoskeletal organisation of endothelial cells of the semilunar heart valves in the developing chick (1986). Anat Embryol, 174, 83 - 89.

For In Vitro Research Use Only

RDI Divison of researchd Industries Intl

San Jose, 95123 CA Snell ave 658

USA

or 408-780-0908

EMAIL:margaret@cellular-research.com

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