rev:        June 20, 2005

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COLLAGEN ANTIBODIES  

(anti-Human and others as indicated)

RDI Divison of researchd Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.


click here for list of pure collagens to use with the below antibodies!

-new Biotin conjugates and smaller sizes now available for many of these antibodies!


mouse anti-human collagen type 1 (human/rabbit)

cat#RDI-COLL1abm-H5    $438.00/100ug

-mIgG2a, clone I-8H5

-suitable for western blot, no cross with other collagens


PRODUCT SPECIFICATION SHEET: rabbit anti-Collagen Type I


PRODUCT: Affinity Purified Anti-Collagen Type I (Rabbit) Minimum

Cross Reaction to Type II, III, IV, V and VI Collagens.

CODE NO.: RDI-600-401-103      $594.00/vial 500ug

                     RDI-600-401-103X   $281.00/vial 100ug

-also available Biotin conjugated cat#RDI-600406103   $344.00.00/100ug

LOT NO:

PHYSICAL STATE: Liquid (Sterile Filtered)

SIZE: 0.5 mg

ANTIBODY CONCENTRATION: 0.86 mg/ml (by UV absorbance at 280 nm)

BUFFER: 0.125M Sodium Borate, 0.075M Sodium Chloride, 0.005M EDTA; ph 8.0

STABILIZER: None

PRESERVATIVE: may contain 0.01% sodium azide

RECOMMENDED DILUTIONS: Western Blots 1:5,000 - 1:10,000

                                                       ELISA 1:4,000 - 1:8,000

                                                       Immunohistochemistry 1:50 - 1:200

APPLICATIONS: ELISA, Western Blotting, Immunochemistry.

STORAGE: Store vial at 4`C before opening. Aliquot and freeze.Avoid cycles of thawing and freezing. Expiration date is six (6) months from opening.


PURITY: This product has been prepared by immunoaffinity chromatography using immobilized antigens. Extensive cross-absorption was performed against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities. Typically less than 1% cross reactivity against other types of collagen was detected using ELISA. Some class specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues.

SPECIFICITY: Antibody reacts with most mammalian Type I collagens and has negligible cross-reactivity with Type II, III, IV, V and VI collagens.

IMMUNOGEN: Collagen Type I from human tissues


NOTE: This product is FOR IN-VITRO Research USE ONLY


Rabbit anti-mouse Collagen Type I   (Mouse,Rat & Human)

cat# RDI-MCOLL1abr  $594.00

Presentation: 100ul neat rabbit sera (no preservative)

Immunogen: Type I collagen purified from fetal mouse

Specificity: Reacts with mouse, rat and human. Recognizes the type 1 collagen as 2 bands at 115kda by reducing SDS-Page. Very slight reactivity with collagen type III.

Uses: -Immunoblotting:1:200-1:500 with   chemiluminescence detection

          -Immunofluorescence:1:20-1:40

          -immunohistochemistry:1:20-1:60, frozen sections require enzymatic or 41 DEG C heat

           treatment to unwind the triple helical structure.

          -immunoprecipitation1:20-1:60

Storage: At first thaw, tighten cap , centrifuge 1-2 minutes 1000rpm to concentrate antibody in vial. Recommend making working aliquots. Avoid frequent freeze thaw cycles. Additional of 0.1% soidum azide or other preservative recommneded if suitable for your application.

References: Romanos, G.E., et al. 1992. J. Peridont. Res. 27, 101.

Background: Collagens are major fibrous structural elements of cartilage, tendon, bone, skin, lung, and blood vessels. Type III collagen consists of three alpha1(III) chains and is most often found in skin, blood vessels, and internal organs.

For Research Use Only


PRODUCT SPECIFICATION SHEET:Rabbit anti-Collagen Type II

PRODUCT: Affinity Purified Anti-Collagen Type II (Rabbit) Minimum Cross Reaction to Type I, III, IV, V and VI Collagens.


CODE NO.: RDI-600-401-104      $594.00/vial 500ug vial

                     RDI-600-401-104X   $281.00/vial 100ug


-also available Biotin conjugated cat#RDI-600406104   $344.00.00/100ug

LOT NO:

PHYSICAL STATE: Liquid (Sterile Filtered)

SIZE: 0.5 mg

ANTIBODY CONCENTRATION: 1.0 mg/ml (by UV absorbance at 280 nm)

BUFFER: 0.125M Sodium Borate, 0.075M Sodium Chloride, 0.005M EDTA; ph 8.0

STABILIZER: None

PRESERVATIVE:  may contain 0.01% sodium azide

RECOMMENDED DILUTIONS: Western Blots 1:5,000 - 1:10,000

                                                       ELISA 1:4,000 - 1:8,000

                                                       Immunohistochemistry 1:50 - 1:200


APPLICATIONS: ELISA, Western Blotting, Immunochemistry.


STORAGE: Store vial at 4`C before opening. Aliquot and freeze.Avoid cycles of thawing and freezing. Expiration date is six (6) months from opening.


PURITY: This product has been prepared by immunoaffinity chromatography using immobilized antigens. Extensive cross-absorption was performed against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities. Typically less than 1% cross reactivity against other types of collagen was detected using ELISA. Some class specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues.


SPECIFICITY: Antibody reacts with most mammalian Type II collagens and has negligible cross-reactivity with Type I, III, IV, V and VI collagens.

IMMUNOGEN: Collagen Type II from human tissues


NOTE: This product is FOR IN-VITRO Research USE ONLY


Mouse anti-Collagen Type II   (human/Mouse/Rat/Bovine/Pig/Chicken)

cat# RDI-COLL2abm      $438.00/100ug

       RDI-COLL2abmBT    $562.00/100ug Biotin labeled

Presentation: Protein G purified antibody in 10mM PBS with 0.2% BSA and 15mM NaN3. (Bulk available without BSA/Azide upon request) concentration 200ug/ml

Isotype: mIgG2ak

Clone: 2B1.5

Reactivity: human, rat, mouse, bovine, pig and chicken (others not tested)

Uses: -Western blot: 1ug/ml 2-3 hours RT

          -histochemistry (frozen and paraffin sections, for formalin fixed tissues, Must using pepsin pretreatment at 1mg/ml

          in Tris-HCL pH2.0 for 15 minutes at room temp or 10 min at 37   DEG C.) approx 2-4ug/ml 60 minutes RT

          -Elisa (order without BSA, add-X to catalog#, ie, RDI- COLL2abm-X, $688.00/200ug)

           -flow cytometry

         -immunofluorescence  1:50-1:100

Positive Control: cartilage in lung or fetus

Localization: cartilage matrix


Background: Recognizes collagen type II and shows no reaction with types I, III, IV, V, VI, IX, X or XI. In western blot, this clone reacts with the TC(b) fragment of pepsin digested type I collagen after digestion with mammalian collagenase. It also reacts with lathyritic type II collagen. The binding epitope is localized to the carboxyl-terminal one quarter of hte type II collagen. It recognizes the ai(II) chain but not the a3(XI) chain, The epitope for clone 2B1.5 Mab is not susceptible to digestion of lathyritic type II collagen with recombinant stromelysin.

reference: The Journal of Histotechnology, 20(3):215,1997.

Storage: 2-8 deg c

For Research Use Only


Mouse anti-Collagen Type II   (human/Mouse/Chicken/Salamander)

cat# RDI-COLL2abm3      $438.00/100ug

        RDI-COLL2abm31    $750.00/200ug

Presentation: Protein G purified antibody in 10mM PBS with 0.2% BSA and 15mM NaN3. (Bulk available without BSA/Azide upon request) concentration 200ug/ml

Isotype: mIgG1

Clone: 6B3

Reactivity: human, mouse, chicken and salamander (others not tested)

Uses: -Western blot: 1ug/ml

--histochemistry (frozen and paraffin sections, for formalin fixed tissues, Must using pepsin pretreatment at 1mg/ml in Tris-HCL pH2.0 for 15 minutes at room temp or 10 min at 37 DEG C.) approx 2-4ug/ml 60 minutes RT

-Elisa (order without BSA, add-X to catalog#, ie, RDI- COLL2abm3-X, $688.00/200ug)

Positive Control: cartilage

Localization: cartilage matrix

Background: Recognizes collagen type II and shows no reaction with types I, or III. In western blot, this clone reacts with the TC(a) fragment of lathyritic type II collagen after digestion with mammalian collagenase. It also reacts with pepsin digested type II collagen. The epitope for clone 6B3 is localized in the triple helix of type II collagen. Immunoblotting of CnBr peptides of collagen II shows that clone 6B3 Mab reacts with CB 11 fragment which is the site of immunohgenic and arthritogenic epitopes alon the intact II molecule. In western blot (but not in Elisa) this Mab recognizes a1(II) as well as a3(Xi) chain both of which have identical primary structure.

Storage: 2-8 deg c

For Research Use Only


Mouse anti-Collagen Type II   (human/Mouse/Chicken)

cat#  RDI-COLL2abm5     $438.00/100ug

         RDI-COLL2abm51   $750.00/200ug

Presentation: Protein G purified antibody in 10mM PBS with 0.2% BSA and 15mM NaN3. (Bulk available without BSA/Azide upon request) concentration 200ug/ml

Isotype: mIgG2ak

Clone: 5B2.5

Reactivity: human, mouse, and chicken (others not tested)

Uses: -Western blot: 1ug/ml

        -immunofluorescence

       -Elisa (order without BSA, add-X to catalog#, ie, RDI- COLL2abm5-X)

Positive Control: cartilage

Localization: cartilage matrix

Background: Recognizes collagen type II and shows no reaction with types I, III, IV, V, VI, IX, X or XI. In western blot, this clone reacts with the TC(a) fragment of lathyritic ype II collagen after digestion with mammalian collagenase. It does not react with pepsin-digested tyep II collagen. The epitope for this Mab is localized to the aminotelopeptide of the type II collagen. Precise mapping of clone 5B2.5 is reported to be GGFDEK, slightly preceding the lysine residue involved in crosslinking. Two germinal residues constitue a key structural element of the epitope. The epitope for this Mab is lost after digestion of lathyritic type II collagen with recombinant stromelysin.

Storage: 2-8 deg c

For Research Use Only


Rabbit anti-rat collagen type 2 (Rat/Human/Mouse)

cat#RDI-COLL2abr    $594.00/500ul

-reacts with rat, mouse and human type II collagen


PRODUCT SPECIFICATION SHEET:Rabbit anti-Collagen Type III


PRODUCT: Affinity Purified Anti-Collagen Type III (Rabbit) Minimum Cross Reaction to Type I, II, IV, V and VI Collagens.

CODE NO.: RDI-600-401-105    $594.00/vial 500ug

                     RDI-600-401-105X  $281.00/vial 100ug

-also available Biotin conjugated cat#RDI-600406105   $344.00/100ug


LOT NO:

PHYSICAL STATE: Liquid (Sterile Filtered)

SIZE: 0.5 mg

ANTIBODY CONCENTRATION: 0.5 mg/ml (by UV absorbance at 280 nm)

BUFFER: 0.125M Sodium Borate, 0.075M Sodium Chloride, 0.005M EDTA; ph 8.0

STABILIZER: None

PRESERVATIVE:  may contain 0.01% sodium azide

RECOMMENDED DILUTIONS: Western Blots 1:5,000 - 1:10,000

                                                       ELISA 1:4,000 - 1:8,000

                                                       Immunohistochemistry 1:50 - 1:200

APPLICATIONS: ELISA, Western Blotting, Immunochemistry.

STORAGE: Store vial at 4`C before opening. Aliquot and freeze.Avoid cycles of thawing and freezing. Expiration date is six (6) months from opening.


PURITY: This product has been prepared by immunoaffinity chromatography using immobilized antigens. Extensive cross-absorption was performed against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities. Typically less than 1% cross reactivity against other types of collagen was detected using ELISA. Some class specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues.

SPECIFICITY: Antibody reacts with most mammalian Type III collagens and has negligible cross-reactivity with Type I, II, IV, V and VI collagens.

IMMUNOGEN: Collagen Type III from human tissues

NOTE: This product is FOR IN-VITRO Research USE ONLY


Rabbit anti-mouse Collagen Type III   (Mouse,Rat & Human)

cat# RDI-MCOLL3abr   $594.00

Presentation: 100ul neat rabbit sera (no preservative)

Immunogen: Type III collagen purified from fetal mouse skin digested with CnBr

Specificity: Reacts with mouse, rat and human. Recognizes the type 3. Does not react with other collagens type 1, 2, 4, 5 or 6.

Uses: -Immunoblotting:1:500-1:2000 with chemiluminescence detection

         -Immunofluorescence:1:50-1:200

         -immunohistochemistry:1:50-1:200, frozen sections require enzymatic or 41 DEG C heat treatment to unwind the triple helical structure.

         -immunoprecipitation 1:20-1:60



Storage: At first thaw, tighten cap , centrifuge 1-2 minutes 1000rpm to concentrate antibody in vial. Recommend making working aliquots. Avoid frequent freeze thaw cycles. Additional of 0.1% soidum azide or other preservative recommneded if suitable for your application.

References: Romanos, G.E., et al. 1992. J. Peridont. Res. 27, 101.

Background: Collagens are major fibrous structural elements of cartilage, tendon, bone, skin, lung, and blood vessels. Type I collagen, a heterotrimer consisting of two a1 chains and one a2 chain, is the most abundant fibrillar collagen. It is preferentially synthesized in bone, skin, and tendon and is the most abundant protein found in these tissues.

For Research Use Only


PRODUCT SPECIFICATION SHEET:Rabbit anti-Collagen Type IV

PRODUCT: Affinity Purified Anti-Collagen Type IV (Rabbit) Minimum

Cross Reaction to Type I, II, III, V and VI Collagens.

CODE NO.: RDI-600-401-106      $594.00/vial 500ug vial

                     RDI-600-401-106X   $281.00/vial 100ug
-also available Biotin conjugated cat#RDI-600406106   $344.00/100ug

LOT NO:

PHYSICAL STATE: Liquid (Sterile Filtered)

SIZE: 0.5 mg

ANTIBODY CONCENTRATION: 6.4 mg/ml (by UV absorbance at 280 nm)

BUFFER: 0.125M Sodium Borate, 0.075M Sodium Chloride, 0.005M EDTA; ph 8.0

STABILIZER: None

PRESERVATIVE: may contain 0.01% sodium azide

RECOMMENDED DILUTIONS: Western Blots 1:5,000 - 1:10,000

                                                       ELISA 1:4,000 - 1:8,000

                                                       Immunohistochemistry 1:50 - 1:200


APPLICATIONS: ELISA, Western Blotting, Immunochemistry.

STORAGE: Store vial at 4`C before opening. Aliquot and freeze.Avoid cycles of thawing and freezing. Expiration date is six (6) months from opening.

PURITY: This product has been prepared by immunoaffinity chromatography using immobilized antigens. Extensive cross-absorption was performed against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities. Typically less than 1% cross reactivity against other types of collagen was detected using ELISA. Some class specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues.

SPECIFICITY: Antibody reacts with most mammalian Type IV collagens and has negligible cross-reactivity with Type I, II, III, V and VI collagens.

IMMUNOGEN: Collagen Type IV from human tissues

NOTE: This product is FOR IN-VITRO Research USE ONLY


Mouse anti-Collagen Type V (COCKTAIL)   (human)  Paraffin Sections

cat# RDI-COLL4abm-COC   $438.00/0.5ml

       RDI-COLL4abm-COC1  $562.00/1ml

Presentation: Tissue culture supernatant with 0.09% sodium azide,

Isotype: mIgG1

Clone: PHM-12 & CIV 22

Reactivity: human (others not tested)

Uses: Immunohistology (Formalin/paraffin) (Use Ab at 1:50-1:100 for 30 min at RT) (Staining of formalin/paraffin tissues is enhanced by digestion of tissue sections with Protease XXV at 1mg/ml PBS for 5 minutes at 37�C

Positive Control: Skin.

Localization: Basement membrane

Background: Collagen IV is a major constituent of the basement membranes along with laminins and enactins. It is composed of � 1(IV) chain and � 2(IV) chain in 2:1 ratio . It can form insoluble fibers with high tensile strength. Antibody to collagen IV is useful in detecting the loss of parts of basement membrane in carcinomas. This MAB cocktail is highly specific to type IV collagen. In kidney, it reacts with glomerular and tubular basement membranes, parts of mesangial matrix and the Bowman�s capsule. It also reacts with basal lamina of capillaries as well as basement membranes in a variety of tissues. This Mab cocktail is excellent for the staining of routine formalin-fixed, paraffin-embedded tissues.

References:

1. Odermatt B F, et. al. 91984) Proc Natl Acad Sci USA, 81:7343-7347.

2. Lee CS; et al. Pathology, 1996, 28(2):135-8.

3. Delaigue O; et al. Graefes Archive for Clinical and Experimental Ophthalmology, 1995 233: 331-8.

Storage: 2-8 deg c

For Research Use Only


Pab to Collagen Type IV

cat#RDI-PRO10760   $375.00/vial

Category Rabbit serum


Ig Class IgG


Immunogen Human placental type IV collagen


Clinical Application/Specificity The antibody is reactive with collagen type IV of basement membranes, and shows a homogeneous staining pattern in all tissues. As neoplastic cells of invasive carcinomas often lack a continuous basement membrane, the antibody is useful to distinguish between non-invasive and invasive lesions. Additionally, it can be used for the differentiation of bullous lesions in dermatopathology. No cross reactivity with other collagens at optimal dilutions. In immunoblotting1, a slight cross reactivity with collagen type V is observed.

Recommended Positive Control for Skin, kidney

Application ELISA

Immunoblotting1

Immunohistochemistry

Suitable for frozen tissue and paraffin* sections; (*proteolytic treatment required; enzyme: pepsin)


Antigen Recognized in species tested so far: Most species incl. human, rat, mouse, rabbit


Working Dilution 1:50 - 1:100


Dilution Buffer PBS with 1% BSA and 0.1% Na-Azide


Incubation Time 1 h at room temperature or over night at 4�C


Storage At 4�C (undiluted) or at -20�C (aliquots)


Volume 0,25 ml

References

1. F.T. Bosman, M. Havenith, and J.P.M. Cleutjens: Basement Membranes in Cancer. Ustrastructural Pathology (1985) 8:291-304.

2. R. Visser, J.M.H. v.d. Beek, M.G. Havenith, J.P.M. Cleutjens & F.T. Bosman: Immunocytochemical detection of basement membrane antigens in the histopathological evaluation of laryngeal dysplasia and neoplasia.

For  In Vitro Research Use Only-Not For Use In Diagnostics


Mab to Collagen Type IV      
cat#RDI-PRO10710    $375.00/vial


Clone 1042


Category Mouse monoclonal, ascites


Ig Class IgG2b


Immunogen Human placental type IV collagen


Clinical Application/Specificity Antibody 1042 is reactive with human collagen type IV of basement membranes, and shows a homogeneous staining pattern in all tissues. As neoplastic cells of invasive carcinomas often lack a continuous basement membrane, the antibody is useful to distinguish between non-invasive and invasive lesions. Additionally, it can be used for the differentiation of bullous lesions in dermatopathology. No cross-reactivity with other collagens.

Recommended Positive Control: for Skin, kidney


Application
� ELISA

� Immunoblotting

� Immunohistochemistry

� Suitable for frozen tissue and paraffin* sections; (*proteolytic treatment required; enzyme: pronase)

Antigen Recognized :Human specific

Working Dilution 1:10 - 1:20


Dilution Buffer PBS with 1% BSA and 0.1% Na-Azide


Incubation Time 1 h at room temperature


Storage At 4�C (undiluted) or at -20�C (aliquots)


Volume 1 ml


References

M.G. Havenitz, J.P.M. Cleutjens, C. Beek, E.v.d.Linden, A.F.P.M. De Goeij, and F.T. Bosman: Human specific anti-type IV collagen monoclonal antibodies, characterization and immunohistochemical applicatiuon. Histochemistry (1987) 87:123-128.

For In Vitro Research Use Only-Not For Use in Diagnostics


PRODUCT SPECIFICATION SHEET:Rabbit anti-Collagen Type V

PRODUCT: Affinity Purified Anti-Collagen Type V (Rabbit) Minimum Cross Reaction to Type I, II, III, IV and VI Collagens.

CODE NO.: RDI-600-401-107        $562.00/vial  500ug

                     RDI-600-401-107X     $281.00/vial 100ug

-also available Biotin conjugated cat#RDI-600406107   $344.00/100ug

LOT NO:

PHYSICAL STATE: Liquid (Sterile Filtered)

SIZE: 0.5 mg

ANTIBODY CONCENTRATION: 1.0 mg/ml (by UV absorbance at 280 nm)

BUFFER: 0.125M Sodium Borate, 0.075M Sodium Chloride, 0.005M EDTA; ph 8.0

STABILIZER: None

PRESERVATIVE: may contain 0.01% sodium azide


RECOMMENDED DILUTIONS: Western Blots 1:5,000 - 1:10,000

                                                       ELISA 1:4,000 - 1:8,000

                                                       Immunohistochemistry 1:50 - 1:200

APPLICATIONS: ELISA, Western Blotting, Immunochemistry.


STORAGE: Store vial at 4`C before opening. Aliquot and freeze.Avoid cycles of thawing and freezing. Expiration date is six (6) months from opening.

PURITY: This product has been prepared by immunoaffinity chromatography using immobilized antigens. Extensive cross-absorption was performed against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities. Typically less than 1% cross reactivity against other types of collagen was detected using ELISA. Some class specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues.


SPECIFICITY: Antibody reacts with most mammalian Type V collagens and has negligible cross-reactivity with Type I, II, III, IV and VI collagens.


IMMUNOGEN: Collagen Type V from human tissues


Rabbit anti-Collagen Type V (Human/Rat)

cat#RDI-COLL5abr  $594.00 /500ul

-slight reactivity with collagen type IV, no cross with Type 1, 3,6,or 7

NOTE: This product is FOR IN-VITRO Research USE ONLY


PRODUCT SPECIFICATION SHEET:Rabbit anti-Collagen Type VI

PRODUCT: Affinity Purified Anti-Collagen Type VI (Rabbit) Minimum

Cross Reaction to Type I, II, IV, V and VI Collagens.


CODE NO.: RDI-600-401-108      $594.00/vial  500ug

                     RDI-600-401-108X   $281.00/vial  100ug
-also available Biotin conjugated cat#RDI-600406108   $344.00/100ug

LOT NO:

PHYSICAL STATE: Liquid (Sterile Filtered)

SIZE: 0.5 mg

ANTIBODY CONCENTRATION: 1.0 mg/ml (by UV absorbance at 280 nm)

BUFFER: 0.125M Sodium Borate, 0.075M Sodium Chloride, 0.005M EDTA; ph 8.0

STABILIZER: None

PRESERVATIVE: may contain 0.01% sodium azide

RECOMMENDED DILUTIONS: Western Blots 1:5,000 - 1:10,000

                                                       ELISA 1:4,000 - 1:8,000

                                                       Immunohistochemistry 1:50 - 1:200

APPLICATIONS: ELISA, Western Blotting, Immunochemistry.

STORAGE: Store vial at 4`C before opening. Aliquot and freeze.Avoid cycles of thawing and freezing. Expiration date is six (6) months from opening.

PURITY: This product has been prepared by immunoaffinity chromatography using immobilized antigens. Extensive cross-absorption was performed against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities. Typically less than 1% cross reactivity against other types of collagen was detected using ELISA. Some classs pecific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues.


SPECIFICITY: Antibody reacts with most mammalian Type VI collagens and has negligible cross-reactivity with Type I, II, III, IV and V collagens.



IMMUNOGEN: Collagen Type VI from human tissues

NOTE: This product is FOR IN-VITRO Research USE ONLY


Mouse Anti-Human COLLAGEN TYPE VII

Human/Monkey/bovine/sheep/pig/guinea pig

cat# RDI-COLL7abm-72          $438.00/200ug in 1ml (w/BSA/Azide)

        RDI-COLL7abm-72XP    $500.00/200ug in 0.2ml (NO BSA or azide)

Presentation: 200ug in 1ml PBS with 1mg/ml BSA and 0.09% sodium azide

Clone: LH7.2

Ig Isotype mIgG1k

Immunogen: Insoluble fractions of human neonatal foreskin epidermal cells.1

Mol. Wt. of Antigen: 250kDa (intact) 150kDa (digested)

Epitope: On non-helical (non-collagenous) C-terminal region of collagen VII and is resistant to collagenase, trypsin, pronase, neuraminidase, chondroitinase, and hyaluronidase.1 However, it is lost by digestion of collagen VII with pepsin.1

SpeciesReactivity: Human, Marmoset, Monkey,Cow, Sheep, Goat, Pig, and Guinea pig (others not tested)

Applications: Electron Microscopy 1

                        Immunofluorescence 1

                      Immunohistology (Acetone-fixed frozen only)1 (Fixation in methanol is not suitable)

                      The optimal dilution for a specific application should be determined by the investigator.

Positive Ctrl: Normal skin.

Cellular Localization: Epidermal basement membrane.

Comments: Mab shows no cross-reaction with Type I, II, III, IV, and V collagens, laminin, and fibronectin. It reacts with the basement membrane zone of stratified squamous epithelia. Ab-1 is useful in differentiating invasive from non-invasive melanoma through clear visualization of appearance and integrity of epidermal basement membrane. This Mab is also helpful in the identification of recessive Dystrophic Epidermolysis Bullosa (RDEB) in which Type VII collagen is defective.

Storage: 4 DEG C. Avoid frequent warming cycles

References:

1. Leigh IM, et. al. Epithelia, 1:17-29, 1987.

2. Burge S; et al. Brit J Dermatol, 1988, 118:629-640.

3. Heagerty AH; et al. J Invest Dermatol 1986, 86:603-5.

4. Leigh IM; et al. J Inve Dermatol 1988, 90(5):639-42.

5. Kirkham N; et al. J Pathol, 1989, 157(3):243-7.

6. Tatnall FM; et al. Brit J Dermatol, 1989, 120:533-9.

7. Shimizu H; et al J Dermatol, 1990, 122(5):577-85.

8. Wetzels RH; et al. Am J Pathol, 1991, 139(2):451-9.

9. Kirkham N; et al. J Pathol, 1989, 159(1):5-6.

For research Use Only Not supplied for use in human diagnostic or therapeutic procedures


rabbit anti-rat collagen type IX (Rat/mouse/human/bovine)

cat#RDI-COLL9abr  $594.00/500ul


rabbit anti-rat collagen type 10 (X)  (Rat/mouse/human)

cat#RDI-COLL10abr  $594.00/500ul

Presentation: 500ul neat rabbit sera (no preservative)

Immunogen: Type X collagen purified from rat chondrosarcoma cells

Reactivity: Reacts with human, mouse, and rat collagen type X. Exhibits slight cross-reactivity to fibronectin and collagen types II and IX. Does not cross react with collagen types I, III, or XI.

Description: Undiluted polyclonal antibody recognizing type X collagen. Collagens are major fibrous structural elements of cartilage, tendon, bone, skin, lung, and blood vessels. Type X collagen, a heterotrimer consisting of three a1(X)subunits, is found in hypertrophic and mineralizing cartilage. Type X collagen provides a transition to bone by allowing removal of hypertrophic cartilage and calcification. Type X collagen is believed to be involved in skeletal growth, maintenance, and repair.

Uses: -Immunoblotting:1:100-1:300 with chemiluminescence detection

         -Immunofluorescence:1:20-1:40

          -immunohistochemistry:1:30-1:60, frozen sections require enzymatic or 41 DEG C heat   treatment to unwind the triple helical   structure.

         -immunoprecipitation1:20-1:60

Storage: At first thaw, tighten cap , centrifuge 1-2 minutes 1000rpm to concentrate antibody in vial. Recommend making working aliquots. Avoid frequent freeze thaw cycles. Additional of 0.1% soidum azide or other preservative recommneded if suitable for your application.

References: -Chung, K.S., et al. 1995 . Dev. Biol. 170, 387.

                     -Kwan, et al. 1986. Biosci. Rep. 6, 155.

                     -Schmid, T.M., and Linsenmayer, T.M. 1985. Dev. Biol. 107, 373.

For research use only, not for diagnostic use.


Mouse anti-Collagen Type 10- (X)   (Chicken)

cat# RDI-COLL10abm        $469.00/100ug

        RDI-COLL10abm1     $750.00/200ug

        RDI-COLL10abmBT  $625.00/100ug Biotin labeled

Presentation: Protein G purified antibody in 10mM PBS with 0.2% BSA and 15mM NaN3. (Bulk available without BSA/Azide upon request) concentration 200ug/ml

Isotype: mIgG1k

Clone: X-AC9

reactivity: chicken , NOT with human (others not tested)

Uses: -histochemistry (frozen and paraffin sections, for formalin fixed tissues, 2-4ug/ml 30 minutes rt [Staining of formalin/paraffin tissues REQUIRES boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by ooling at RT for 20 min. Staining is further enhanced if heat-induced epitope retrieval is followed by digestion of tissue sections with 0.05% hyaluronidase at 37 0 C for 30 min].

-Elisa (order without BSA, add-X to catalog#, ie, RDI-COLL10abm-X, $812.00/200ug)

-immunofluorescence 2-10ug/ml

Pos Cntrl: chicken cartilage

Localization: cartilage matrix

Background: Type X collagen is a non-fibrillar, short-chain, network-forming collagen found in fetal   hypertrophic cartilage in the growth zones of long   bones, vertebrae, and ribs. It is a short collagen with a triple helical core half the length of the fibril-forming collagen type I, II, and III and a globular domain at the carboxyl end (NC-1 domain) through which the molecules assemble into an hexagonal meshwork. The function of collagen X, a unique homotrimer synthesised by hypertrophicchondrocytes, is not known but its localization and transient expression at sites of calcification suggest that it is likely to be associated with events in the early stages of endochondral bone formation.

References: 1. Schmid T and Linsenmayer TF. J Cell Biol, 1985;   100:598-605.

Storage: 2-8 deg c

For Research Use Only


NEW!

Pure Collagens for Control Use:   click here for specs:

-for in vitro research use only-not for use in or on humans or animals-not for use in diagnostics

Human Type I Collagen      cat#RDI-9001103      $344.00/500ug

Human Type II Collagen     cat#RDI-9001104      $594.00/100ug

Human Type III Collagen    cat#RDI-9001105      $344.00/500ug

Human Type IV Collagen    cat#RDI-9001106      $344.00/500ug

Human Type V Collagen     cat#RDI-9001107      $344.00/500ug

Human Type VI Collagen    cat#RDI-9001108      $344.00/500ug

-see also our antibodies to collagen Type I, II, III, IV, V, VI and VII(monoclonal), and related laminin and

fibronectin.


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email: margaret@cellular-research.com

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