rev: January 20, 2004

HOME (index page)

Return ( alphabetcial antibody page)

Return  (cytokeratin data page)


CYTOKERATIN   ANTIBODIES  

(anti-Human and others as indicated)

RDI Divison of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.

ANTI-CYTOKERATIN  TYPE I


Mab to Cytokeratin Type I

RDI-PRO61804    $438.00  (replaces RDI-CBL215)

Clone Determination         AE 1

Category                            Mouse monoclonal

Immunoglobulin Class       IgG1

Purification                        Ion-exchange chromatography

Antigen                              Human epidermal keratin

Specificity                        AE 1 represents an excellent marker for distinguishing carcinoma from

                                        non-epithelial tumors. Tumors specifically detected: All epithelium-derived tumors.

Application Suitable for   · Frozen and paraffin-embedded tissue

                                        · Cytological material

                                         · Immunoblotting (Western)

Polypeptide Reacting      Mr 40 000, Mr 48 000 and Mr 50 000 polypeptides of human epithelial proteins (cytokeratins nos. 19, 16, 14) and most other acidic (type I) cytokeratins

Antigen Recognized in                Human, monkey, rabbit, mouse, rat,

Species (tested so far)                bovine, chicken

Reactivities on Cultured             MCF-7, A-431, RT 112

Cell Lines (tested so far)

Working Dilution                         Dilute approx. 1:200 with PBS pH 7.4 for immunohistochemical application; optimal working dilution should be determined by serial dilution prior to application

Incubation Time 1h at RT; extended with Paraffin

Pretreatment With paraffin-embedded sections, protease pretreatment is recommended.

Storage At 2-8°C

Quantity 200 µg (1 mg/mL) in PBS with 0.1% NaN3

References

Battifora H, Sheibani K, Tubbs RR, Kopinski MI and Sun TT: Anti-keratin antibodies in tumor diagnosis: Distinction between seminoma and embryonal carcinoma. Cancer 54, 843-848 (1984)

Eichner R, Bonitz P and Sun TT.: Classification of epidermal keratins according to their immunoreactivity, isoelectric point and mode of expression. J Cell Biol 98, 1388-1396 (1984)

Garin-Chesa P, Rettig J and Melamed R: Expression of cytokeratin in normal and neoplastic colonic epithelial cells: Implications for cellular differentiation and carcinogenesis. Am J Surg Pathol 10, 829-835 (1986)

Nelson WG, Battifora H, Santana H and Sun TT: Specific keratins as molecular markers for neoplasms with a stratified epithelial origin. Cancer Res. 44, 1600-1603 (1984)

Nelson WG and Sun TT: The 50- and 58-kD keratin classes as molecular markers for stratified squamous epithelia: Cell culture studies. J Cell Biol 97, 244-251 (1983)

Moll R, Franke WW, Schiller DL, Geiger B and Krepler R: The catalog of human cytokeratins: Patterns of expression in normal epithelia, tumors and cultured cells. Cell 31, 11-24 (1982)

Shi SR, Goodman ML, Chan AK, Pilch BZ, Chen LB and Sun TT: Immunohistochemical study of nasopharyngeal carcinoma using monoclonal anti-keratin antibodies. Am J Pathol 117, 53-63 (1984)

Spagnolo DV, Michie SA, Crabtree GS, Warnke RA and Rouse RV: Monoclonal anti-keratin (AE1) reactivity in routinely processed tissue from 166 human neoplasms. Am J Clin Pathol 84, 697-704 (1985)

Sun TT, Eichner R, Cooper D, Schermer A, Nelson WG and Weiss RA: Classification, expression and possible mechanisms of evolution of mammalian epithelial keratins: A unifying model. The Cancer Cell 1, 169-176 (1984)

Sun TT, Eichner RA, Nelson WG, Tseng SCG, Weiss RA, Jarvinen M and Woodcock-Mitchell J: Keratin classes: Molecular markers for different types of epithelial differentiation. J Invest Dermatol 81, 109-115 (1983)

Sun TT, Tseng SCG, Huang AJW, Cooper D, Lynch MH, Weiss RA, Eichner RA and Schermer A: Monoclonal antibody studies of keratin expression: A review. In: Intermediate Filaments. The New York Acad Sci (E Wang, D Fischmann, R Liem, and TT Sun, eds.) 455, 307-329 (1985)

Tseng SCG, Jarvinen M, Nelson WG, Huang JW, Woodcock-Mitchell J and Sun TT: Correlation of specific keratins with different types of epithelial differentiation: Monoclonal antibody studies. Cell 30, 361-372 (1982)

Woodcock-Mitchell J, Eichner R, Nelson WG and Sun TT: Immunolocalization of keratin polypeptides in human epidermis using monoclonal antibodies. J Cell Biol 95, 580-588 (1982)

Weiss RA, Eichner R and Sun TT.: Monoclonal antibody analysis of keratin expression in epidermal diseases: A 48 kD and a 56 kD keratin as molecular markers for hyperproliferating keratinocytes. J Cell Biol 98, 1397-1406 (1984)

Weiss RA, Guillet GY, Freedberg IM, Small EA, Farmer ER, Weiss M and Sun TT: The use of monoclonal antibody to keratin in human epidermal disease: Alterations in immunohistochemical staining pattern. J Invest Dermatol 81, 2279-2286 (1983)


RDI Divison of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

ordering terms

RETURN(cytokeratin page)