rev: February 22, 1999

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CYTOKERATIN   ANTIBODIES  

(anti-Human and others as indicated)

RDI Divison of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.


ANTI-CYTOKERATIN  20



MOUSE MONOCLONAL ANTIBODY TO: HUMAN CYTOKERATIN 20

PRODUCT CODE   RDI-CBL208    $469.00/vial

CLONE         Ks20.8

ISOTYPE      IgG2a

IMMUNIZING ANTIGEN   Electrophoretically purified cytokeratin 20 from human intestinal mucosa

SOURCE     Mouse ascitic fluid

PURIFICATION METHOD    Hydrophobic interaction chromatography

SPECIFICITY   The antibody is specific for the 46 kDa polypeptide (cytokeratin 20). It represents an excellent marker for certain carcinomas such as adenocarcinoma of the colon, transitional cell carcinoma of the bladder and Merkel cell skin tumours. The antibody can be used in the detection of intestinal and gastric foveolar epithelium, urothelial cells and Merkel cells of the epidermis as well as tumours of their origin (eg. primary and metastatic colorectal cancer).

Adenocarcinomas of the breast and lung also neuroendocrine tumours of the lung are essentially negative.

APPLICATIONS

    *Cytological material

    *Western blotting

     *Suitable for staining paraffin wax embedded tissue following enzyme digestion and incubation for 1 hour at room temperature

REFERENCES

Moll, R. et al. J. Cell Biol. 111, 567-580 (1990)

Moll, I. & Moll, R. Arch. Dermatol. Res. 283, 300-309 (1991)

Moll, R. et al. Am. J. Path. 140, 427-447 (1992)

LOT SPECIFIC DATAPRESENTATION The monoclonal is presented as 50µg of lyophilysed material (contains 0.5% BSA and 0.09% NaN3). Reconstitute with 1ml distilled water. The working dilution is 5µg/ml but we recommend that each laboratory determine an optimum working titre for use in its particular application.

STORAGE For use within 1 month of purchase store at +4oC, for long term storage aliquot antibody into small volumes and store at -20oC.

-see also rec human cytokeratin 20 antigen

For research use only. Not supplied for use in human diagnostic or therapeutic procedures


RDI Divison of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

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