rev: January 14, 2001

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CYTOKERATIN   ANTIBODIES  

(anti-Human and others as indicated)

RDI Divison of researchd Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.

ANTI-CYTOKERATIN  TYPE 14

MOUSE MONOCLONAL ANTIBODY TO: HUMAN CYTOKERATIN 14

PRODUCT CODE   RDI-CBL197 $344.00/vial

-also availabeleFITC conjugated: cat#RDI-CBL197FT  $375.00/100T

CLONE   LL002

ISOTYPE   IgG3

IMMUNIZING ANTIGEN  Thyroglobulin conjugated synthetic peptide of extreme carboxy terminal of human keratin 14 (last 15 residues)

FUSION PARTNER  NS1 myeloma cell line

SOURCE  Tissue culture supernatant

PURIFICATION METHOD  Ammonium sulphate precipitation followed by ion exchange chromatography

SPECIFICITY

Cytokeratin 14, a type I intermediate filament, is one of the two cytokeratins that distinguish stratifying epithelial cells types from simple epithelial cell types. Thus, cytokeratin 14 is expressed by stratifying/keratinocyte cell types but not by simple epithelial cells.

APPLICATIONS

*Suitable for staining formalin fixed wax sections or frozen tissues. In order to obtain 100% consistency in staining it is considered essential to either microwave treat fixed tissue sections or alternatively use the more recently recommended high temperature antigen unmasking technique. Prior exposure of fixed tissues to enzyme (trypsin) digestion is deleterious to epitope expression. The fixative recommended for use with CBL 197 is neutral buffered formal saline

*Western blotting (band 50kda under reducing conditions)

REFERENCES

Moll, R. et al. Cell 31, 11-14 (1982)

Purkis, P. E. et al. J. Cell Sci. 97, 39-50 (1990)

PRESENTATION The monoclonal is presented at a concentration of 200µg/2ml in phosphate buffered saline containing 10mM sodium azide and 1% bovine serum albumin. We recommend that each laboratory determine an optimum working titre for use in its particular application.

STORAGE For use within 1 month of purchase store at +4oC, for long term storage aliquot antibody into small volumes and store at -20oC.

For Research Use Only. Not supplied for use in diagnostic or therapeutic procedures.

See also recombinant human Cytokeratin 14 cat#RDI-PRO62218   $438.00/100ug

                                                                      cat#RDI-PRO62018   $750.00/250ug

For research use only. Not supplied for use in human diagnostic or therapeutic procedures

San Jose, 95123 CA Snell ave 658

USA

or (800) 370-2222

EMAIL:margaret@cellular-research.com

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