rev: October 10, 2000
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CYTOKERATIN
ANTIBODIES
(anti-Human and others as indicated)
RDI Divison of researchd Industries Intl offers a wide line of antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.
PRODUCT CODE RDI-CBL194 $375.00
-also available FITC conjugated cat#RDI-CBL194 $375.00/100T
CLONE LP5K
ISOTYPE IgG2b
IMMUNIZING ANTIGEN Cytoskeletal proteins from cultured HeLa cells
FUSION PARTNER Sp2/0
SOURCE Tissue culture supernatant
PURIFICATION METHOD Ammonium sulphate precipitation followed by ion exchange chromatography
SPECIFICITY The antibody reacts with cytokeratin 7 which has been found in columnar and glandular epithelium of the lung, cervix, breast, bile ducts and larger collecting ducts of the kidney. Occasionally staining of blood vessel cell walls, particularly endothelial cells, may be observed.
APPLICATIONS *Suitable for staining frozen sections and paraffin wax embedded tissue. Protease treatment of paraffin embedded sections is required prior to antibody application
*Cytological material
*Western blotting
REFERENCES
Osborn, M. et al. Lab. Invest. 55, 497-504 (1986)
Moll, R. et al Cell 31, 11-24 (1982)
Ramaekers, F. et al. Path. 11, 14-17 (1990)
Vojtesek, B. et al. Neoplasma 37, 333-42 (1989)
PRESENTATION The monoclonal is presented at a concentration of 200µg/2ml in phosphate buffered saline containing 10mM sodium azide and 1% bovine serum albumin. We recommend that each laboratory determine an optimum working titre for use in its particular application.
STORAGE For use within 1 month of purchase store at +4 oC, for long term storage aliquot antibody into small volumes and store at -20oC.
For research use only. Not supplied for use in human diagnostic or therapeutic procedures
RDI Divison of researchd Industries Intl
San Jose, 95123 CA Snell ave 658
USA
or 408-780-0908
EMAIL:margaret@cellular-research.com
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