rev: October 10, 2000

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CYTOKERATIN   ANTIBODIES  

(anti-Human and others as indicated)

RDI Divison of researchd Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.


ANTI-CYTOKERATIN  TYPE 7  (see also other cytokeratin 7 clones)
MOUSE MONOCLONAL   ANTIBODY TO: HUMAN CYTOKERATIN 7

PRODUCT CODE                RDI-CBL194  $375.00

     -also available FITC conjugated cat#RDI-CBL194  $375.00/100T

CLONE                                  LP5K

ISOTYPE                               IgG2b

IMMUNIZING ANTIGEN     Cytoskeletal proteins from cultured HeLa cells

FUSION PARTNER               Sp2/0

SOURCE                                 Tissue culture supernatant

PURIFICATION METHOD   Ammonium sulphate precipitation followed by ion exchange chromatography

SPECIFICITY                       The antibody reacts with cytokeratin 7 which has been found in columnar and glandular epithelium of the lung, cervix, breast, bile ducts and larger collecting ducts of the kidney. Occasionally staining of blood vessel cell walls, particularly endothelial cells, may be observed.

APPLICATIONS               *Suitable for staining frozen sections and paraffin wax embedded tissue. Protease treatment of paraffin embedded sections is required prior to antibody application

                                          *Cytological material

                                           *Western blotting

REFERENCES

Osborn, M. et al. Lab. Invest. 55, 497-504 (1986)

Moll, R. et al Cell 31, 11-24 (1982)

Ramaekers, F. et al. Path. 11, 14-17 (1990)

Vojtesek, B. et al. Neoplasma 37, 333-42 (1989)

PRESENTATION The monoclonal is presented at a concentration of 200µg/2ml in phosphate buffered saline containing 10mM sodium azide and 1% bovine serum albumin. We recommend that each laboratory determine an optimum working titre for use in its particular application.

STORAGE For use within 1 month of purchase store at +4 oC, for long term storage aliquot antibody into small volumes and store at -20oC.

For research use only. Not supplied for use in human diagnostic or therapeutic procedures


RDI Divison of researchd Industries Intl

San Jose, 95123 CA Snell ave 658

USA

or (800) 370-2222

EMAIL:margaret@cellular-research.com

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