rev: December 20, 2004
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CYTOKERATIN
ANTIBODIES
(anti-Human and others as indicated)
RDI Division of Fitzgerald Industries Intl offers a wide line of antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.
ANTI-CYTOKERATIN TYPE 18
MOUSE MONOCLONAL ANTIBODY TO: HUMAN CYTOKERATIN 18:FITC Conjugated
PRODUCT CODE cat#RDI-CBL185FT $375.00/100T
CLONE DC-10
ISOTYPE IgG1
IMMUNIZING
ANTIGEN PMC-42 human breast carcinoma cells
FUSION PARTNER P3-x63-Ag8.653 myeloma cells
SOURCE Tissue culture supernatant
PURIFICATION METHOD Ammonium sulphate precipitation followed by ion exchange chromatography
SPECIFICITY This antibody reacts with human cytokeratin 18 (45 kDa peptide)
APPLICATIONSRDI-CBL185 CONT'D *Western blotting*Immunocytochemistry on: - methanol or acetone fixed culture epithelial cells - frozen and methacarn fixed tissue sections - formalin fixed tissue sections, following microwaving antigen retrieval
REFERENCES Hybridoma 7, 495-504 (1988)Int.J. Cancer Suppl. 3, 50-55 (1988)Folin Biol. 35, 373-382 (1989)J. Tumor Marker Oncol. 5, 219 (1990)
LOT SPECIFIC DATA
PRESENTATION The monoclonal is presented at a concentration of 1ooT in 1ml in phosphate buffered saline containing 10mM sodium azide and 1% bovine serum albumin. We recommend that each laboratory determine an optimum working titre for use in its particular application.
STORAGE For use within 1 month of purchase store at +4oC, for long term storage aliquot antibody into small volumes and store at -20oC.
For research use only. Not supplied for use in human diagnostic or therapeutic procedures
34 Junction Square Drive
Concord MA 01742-3049
USA
phone (978) 371-6446 or (800) 370-2222
fax (978) 371-2266
EMAIL:antibodies@fitzgerald-fii.com
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