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CYTOKERATIN   ANTIBODIES  

(anti-Human and others as indicated)

RDI Divison of researchd Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.

ANTI-CYTOKERATIN  TYPE 18

cat#RDI-PRO61028          $375.00/vial

Clone Determination          Ks 18.04

Category                           Mouse monoclonal

Immunoglobulin Class       IgG1

Purification                        Protein A (recombinant)

Antigen                               Human Cytokeratin 18 from HeLa cytoskeletal preparation

Specificity                         Ks 18.04 represents an excellent marker to discriminate simple epithelia from those of different origin

Application                     Suitable for frozen and parrafin embeded tissue Cytological material

                                         Immunoblotting (Western)

                                         microwave release recommended for paraffin sections

Storage                            At 2-8 DEG C one year after reconstitution

Quantity                           50 µg (lyoph.) ; contains 0.5% BSA and 0.09% NaN3

Polypeptide Reacting     Mr 45 000 polypeptide ( human cytokeratin 18) of all simple type epithelia and basal cells of many squamous, nonepidermal epithelia

Antigen Recognized in            Human, mouse, rat, bovine, pig, dog,

Species (tested so far)             hamster, sheep, fish (trout)

Reactivities on Cultured          HeLa and MCF-7

Cell Lines (tested so far)

Tumors Specifically Detected   All tested adeno Ca.; mammary CA, urinary bladder CA, undiff CA, cervix CA,

                                                  h epatocellular CA.

Working Dilution                    After reconstitution with 1 ml dist. water, dilute 1:20 with PBS, pH 7.4 for use in

                                                 i mmunohistochemistry

Incubation Time                       1 h at 37 DEG C (extended with paraffin (suggest overnight at 2-8 DEG C)

References:

Bartek,J., Vojtesek,B., Staskova,Z., Bartkova,J.,Kerekes,Z. Rejthar,A. and Kovarik,J.: A series of 14 new monoclonal antibodies to keratins: Characterization and value in diagnostic histopathology.J. Pathol.(1991) in press.

Heid,H., Vojtesek,B., Bruder,G., Kaufman, M., Stackova,Z., Kovarik,J.,and Franke W.W.: Cell type heterogeneity of mammary gland epithelial and breast carcinoma cells as revealed by cytokeratin expression. Differentiation (1991), submitted.

Layerova,L.,Kovarik,J.,Bartek,J.,Rejthar,A. and Vojtesk, B.: Novel monoclonal antibodies defining epitope of human cytokeratin 18 molecule. Hybridoma 7, 495-504 (1988).

Markl,J., Winter,S. and Franke,W.W." The catalog and the expression complexity oof cytokeratins in a lower verte-brate: Biochemical identification of cytokerations in a teleost fish, the rainbow trout. Eur.J.Cell Ciol.50, 1-16 (1989).

Moll,R., Franke, W.W., Schiller, D.L.,Geiger, B. and Krepler, R.: The catalog of human cytokeratins: Patterns of expression in normal epithelia, tumors and cultured cells 31, 11-24 (1982).

Gomi,H.,Yokoyama,T., Fujmoto,K., Ikeda,T., Katoh,A.,Itoh, T, and Itohara,S.: Mice Devoid of the Glial Fibrillary  Acidic Protein Develop Normally and Are Susceptible to Scrapie Prions. Neuron,Vol. 14,29-41 (1995).

also available as 5ml TCS cat#RDI-PRO65028  $188.00/vial  $156.00/vial 3+

For Research Use Only

RDI Divison of researchd Industries Intl

San Jose, 95123 CA Snell ave 658

USA

or 408-780-0908

EMAIL:margaret@cellular-research.com

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