rev: February 25, 2005
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CYTOKERATIN
ANTIBODIES
(anti-Human and others as indicated)
RDI Division of Fitzgerald Industries Intl offers a wide line of antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.
ANTI-CYTOKERATIN TYPE 8,18
see other cytokertatin antibodies from the main list
Cat#RDI-PRO10502 $375.00
Clone
NCL-5D3
Category Mouse
monoclonal
Form/Purification Ascites
Ig Class IgG2a
Immunogen Isolated from human breast cancer carcinoma cell line MCF-7
Description/
Specificity The antibody recognizes all simple epithelia including glandular epithelia. It does not react with stratified squamous epithelium. Useful for the differentiation between carcinomas and lymphomas. NCL 5D3 is directed against the the 52.2 kD and 45 kD cytokeratins (nos. 8, 18 in the Moll-catalogue) in immunoblotting.
Recommended for Positive Control Glandular epithelium
Application · Immunohistochemistry
· Immunoblotting
· Cell suspensions
· Suitable for frozen and paraffin-embedded tissue
Proteolytic treatment with pepsin or pronase
Antigen Recognized Human, no significant reaction with mouse or rat in Species (tested so far)
Working Dilution 1:5 - 1:10, dilute immediately before use
Dilution Buffer PBS with 1% BSA and 0.1% Na-Azide
Storage At 2-8°C (undiluted) or at -20°C (aliquots)
Volume 1 ml
References
Angus B, et al: NCL-5D3. A new monoclonal antibody recognizing low molecular weight cytokeratins effective for immunohistochemistry using fixed paraffin-embedded tissue. J Pathol 153, 377-384 (1987)
Waseem.A et al, Biochemistry 43, 1283-1295 (2004)
For research use only. Not supplied for use in human diagnostic or therapeutic procedures
RDI Division of Fitzgerald Industries Intl
34 Junction Square Drive
Concord MA 01742-3049
USA
phone (978) 371-6446 or (800) 370-2222
fax (978) 371-2266
EMAIL:antibodies@fitzgerald-fii.com
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