rev:      February 25, 2005

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CYTOKERATIN   ANTIBODIES  

(anti-Human and others as indicated)

RDI Divison of researchd Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.


ANTI-CYTOKERATIN  TYPE 8,18

see other cytokertatin antibodies from the main list



Mab to Cytokeratin 8, 18

Cat#RDI-PRO10502    $375.00

Clone                      NCL-5D3

Category                 Mouse monoclonal

Form/Purification   Ascites

Ig Class                   IgG2a

Immunogen             Isolated from human breast cancer carcinoma cell line MCF-7

Description/

Specificity            The antibody recognizes all simple epithelia including glandular epithelia. It does not react with stratified squamous epithelium. Useful for the differentiation between carcinomas and lymphomas. NCL 5D3 is directed against the the 52.2 kD and 45 kD cytokeratins (nos. 8, 18 in the Moll-catalogue) in immunoblotting.

Recommended for  Positive Control    Glandular epithelium

Application · Immunohistochemistry

                   · Immunoblotting

                   · Cell suspensions

                  · Suitable for frozen and paraffin-embedded tissue

                  Proteolytic treatment with pepsin or pronase

Antigen Recognized Human, no significant reaction with mouse or rat in Species (tested so far)

Working Dilution    1:5 - 1:10, dilute immediately before use

Dilution Buffer      PBS with 1% BSA and 0.1% Na-Azide

Storage                 At 2-8°C (undiluted) or at -20°C (aliquots)

Volume                  1 ml

References

Angus B, et al: NCL-5D3. A new monoclonal antibody recognizing low molecular weight cytokeratins effective for immunohistochemistry using fixed paraffin-embedded tissue. J Pathol 153, 377-384 (1987)

Waseem.A et al, Biochemistry 43, 1283-1295 (2004)

For research use only. Not supplied for use in human diagnostic or therapeutic procedures


RDI Divison of researchd Industries Intl

San Jose, 95123 CA Snell ave 658

USA

or (800) 370-2222

EMAIL:margaret@cellular-research.com

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