rev:   October 5, 2004

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ANTIBODIES  

(anti-Human and others as indicated)

RDI Divison of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.


CA 2+ ATPase isoforms (1, 2, 3 & 4)
Rabbit anti-Ca2+ -ATPase, isoform 1

Code#: RDI-PMCA1abr   $625.00/1 vial

Form: Lyophilized whole serum (no preservatives).

Quantity: 200 ul.

Reconstitution: with 200 ul of bidistilled water.

Description: The antiserum against Ca2+ -ATPase, isoform 1, was produced in rabbits by immunization with the N-terminal sequence-specific peptide of the human pump as epitope. It recognizes the corresponding proteins in human, rat and mouse tissue. The antibody was evaluated for specificity and potency : a) by Biotin-Avidin labelling of cryostate-sections of 4% para-formaldehyde fixed tissue and b) by immunoenzymatic labelling of Western blots. It can also be used for immunoprecipitation. The serum was inactivated at 50'C for 30 minutes.

Working dilutions: Immunohistochemistry: 1: 1'000 - 1:2'000.

                               Immunoblots: 1: 1'000 - 1:2'000.

For immunohistochemistry and immunoblots the titer was determined by using the avidin-biotin method. We recommend that the optimal dilutions be determined by titration experiments.

Storage: After reconstitution freeze in small aliquots (e.g. 1ul) and keep at -80'C (or at least - 20'C). For continuous use keep at 4'C (with 0.01% Na-azide). Avoid repeated freezing and thawing.

References:

a) Stauffer T.P., Guerini D., Carafoli E. (1995) Tissue distribution of the four gene products of the plasma- membrane Ca2+ -pump. A study using specific antibodies J. Biol. Chem. 19: 12184-12190.

b) Stauffer T.P., Guerini D., Celio M,R., Carafoli E. (1997) Immunolocalization of the plasma membrane Ca2+- pump isoforms in the rat brain. Brain Res. 748:21-29.

For Research Use Only


Rabbit anti-Ca2+ -ATPase, isoform 2

Code#: RDI-PMCA2abr   $625.00/1 vial  

Form: Lyophilized whole serum (no preservatives).

Quantity: 200 ul.

Reconstitution: with 200 ul of bidistilled water.

Description: The antiserum against Ca2+ -ATPase, isoform 2, was produced in rabbits by immunization with the N-terminal sequence-specific peptide of the human pump as epitope. It recognizes the corresponding proteins in human, rat and mouse tissue. The antibody was evaluated for specificity and potency : a) by Biotin-Avidin labelling of cryostate-sections of 4% para-formaldehyde fixed tissue and b) by immunoenzymatic labelling of Western blots. It can also be used for immunoprecipitation. The serum was inactivated at 50'C for 30 minutes.

Working dilutions: Immunohistochemistry: 1: 1'000 - 1:2'000.

                               Immunoblots: 1: 1'000 - 1:2'000.

For immunohistochemistry and immunoblots the titer was determined by using the avidin-biotin method. We recommend that the optimal dilutions be determined by titration experiments.

Storage: After reconstitution freeze in small aliquots (e.g. 1ul) and keep at -80'C (or at least - 20'C). For continuous use keep at 4'C (with 0.01% Na-azide). Avoid repeated freezing and thawing.

References:

a) Stauffer T.P., Guerini D., Carafoli E. (1995) Tissue distribution of the four gene products of the plasma- membrane Ca2+ -pump. A study using specific antibodies J. Biol. Chem. 19: 12184-12190.

b) Stauffer T.P., Guerini D., Celio M,R., Carafoli E. (1997) Immunolocalization of the plasma membrane Ca2+- pump isoforms in the rat brain. Brain Res. 748:21-29.

For Research Use Only


Rabbit anti-Ca2+ -ATPase, isoform 3

Code#: RDI-PMCA3abr   $625.00/1 vial

Form: Lyophilized whole serum (no preservatives).

Quantity: 200 ul.

Reconstitution: with 200 ul of bidistilled water.

Description: The antiserum against Ca2+ -ATPase, isoform 3, was produced in rabbits by immunization with the N-terminal sequence-specific peptide of the human pump as epitope. It recognizes the corresponding proteins in human, rat and mouse tissue. The antibody was evaluated for specificity and potency : a) by Biotin-Avidin labelling of cryostate-sections of 4% para-formaldehyde fixed tissue and b) by immunoenzymatic labelling of Western blots. It can also be used for immunoprecipitation. The serum was inactivated at 50'C for 30 minutes.

Working dilutions: Immunohistochemistry: 1: 1'000 - 1:2'000.

                               Immunoblots: 1: 1'000 - 1:2'000.

For immunohistochemistry and immunoblots the titer was determined by using the avidin-biotin method. We recommend that the optimal dilutions be determined by titration experiments.

Storage: After reconstitution freeze in small aliquots (e.g. 1ul) and keep at -80'C (or at least - 20'C). For continuous use keep at 4'C (with 0.01% Na-azide). Avoid repeated freezing and thawing.

References:

a) Stauffer T.P., Guerini D., Carafoli E. (1995) Tissue distribution of the four gene products of the plasma- membrane Ca2+ -pump. A study using specific antibodies J. Biol. Chem. 19: 12184-12190.

b) Stauffer T.P., Guerini D., Celio M,R., Carafoli E. (1997) Immunolocalization of the plasma membrane Ca2+- pump isoforms in the rat brain. Brain Res. 748:21-29.

For Research Use Only


Rabbit anti-Ca2+ -ATPase, isoform 4

Code#: RDI-PMCA4abr   $625.00/1 vial  

Form: Lyophilized whole serum (no preservatives).

Quantity: 200 ul.

Reconstitution: with 200 ul of bidistilled water.

Description: The antiserum against Ca2+ -ATPase, isoform 4, was produced in rabbits by immunization with the N-terminal sequence-specific peptide of the human pump as epitope. It recognizes the corresponding proteins in human, rat and mouse tissue. The antibody was evaluated for specificity and potency : a) by Biotin-Avidin labelling of cryostate-sections of 4% para-formaldehyde fixed tissue and b) by immunoenzymatic labelling of Western blots. It can also be used for immunoprecipitation. The serum was inactivated at 50'C for 30 minutes.

Working dilutions: Immunohistochemistry: 1: 1'000 - 1:2'000.

                               Immunoblots: 1: 1'000 - 1:2'000.

For immunohistochemistry and immunoblots the titer was determined by using the avidin-biotin method. We recommend that the optimal dilutions be determined by titration experiments.

Storage: After reconstitution freeze in small aliquots (e.g. 1ul) and keep at -80'C (or at least - 20'C). For continuous use keep at 4'C (with 0.01% Na-azide). Avoid repeated freezing and thawing.

References:

a) Stauffer T.P., Guerini D., Carafoli E. (1995) Tissue distribution of the four gene products of the plasma- membrane Ca2+ -pump. A study using specific antibodies J. Biol. Chem. 19: 12184-12190.

b) Stauffer T.P., Guerini D., Celio M,R., Carafoli E. (1997) Immunolocalization of the plasma membrane Ca2+- pump isoforms in the rat brain. Brain Res. 748:21-29.

For Research Use Only


RDI Divison of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

Ordering terms

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