rev:  January 11, 2004

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ANTIBODIES  

(anti-Human and others as indicated)
RDI Divison of researchd Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.


Mouse anti-CA ATPase SERCA 2 Monoclonal ab

cat# RDI-CATP/2D8        $469.00/vial

Background: The ATP pumps that reside in intracellular organelles are encoded by a family of structurally related enzymes, termed the sarcoplasmic or endoplasmic reticulin CA+ (SERCA) ATPases. The Serca2 gene is subject to tissue dependent processing which is responsible for the generation of Serca2a muscle-specific isoform expressed in type I (slow) skeletal, cardiac and smooth muscle and the Serca2b isoform expressed in all cells types.

isotype: mIgG1

clone: IID8

package: 100ul of diluted ascites fluid with 0.05% sodium azide added

antigen: produced from a fusion of NS-1 myeloma cells and spleen cells from Balb/c mice immunized with purified canine sarcoplasmic reticulum.

Specificity: recognizes the SERCA2 ATPAse but does not discriminate between the two isoforms (2a or 2b).

Human: Serca 2a, 2b, Type 1 (slow), cardiac

Canine: Serca 2a,2b , type I (slow) ,cardiac

rabbit: Serca2a, 2b, Type I (slow),  cardiac , smooth muscle

Pig:      Serca2b, neuronal, liver

G. Pig:  Serca2a, 2b, type I (slow)

Rat: 0NLY WORKS FOR HISTOCHEMISTRY PURPOSES

Serca2a, 2b, Type I (slow), cardiac, neuronal

Use:

-Western blot 1:2500 recognizes approx 110Kda band

-Immunofluorescence: 1:500 strongly labels the entire type I (slow) myofiber consistent with SR localization.

-histochemistry: has been used to detect serca2b ATPase in the cerebrum and cerebellum of rat and pig.

Storage: Store at -20 DEG C upon receipt. Avoid frequent freeze thaw cycles. Recommend aliquoting. BEFORE OPENING, suggest centrifuging for 1 minute at 1000 RPM to concentrate material in vial.


References: -A.O. Jorgensen et al., Cell Motility and the Cytoskeleton, 9:164-174,. 1988


Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.
It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We arenot responsible for any patent infringements that might result with the use of or derivation of this product.


Also available: antibodies to NA K ATPase and CA ATPASE (sera 1 , serca 2 and serca 3). Please inquire.


RDI Divison of researchd Industries Intl

San Jose, 95123 CA Snell ave 658

USA

or (800) 370-2222

EMAIL:margaret@cellular-research.com

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