rev:January 11, 2004

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ANTIBODIES  

(anti-Human and others as indicated)

RDI Divison of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.


Mouse anti-CA ATPase SERCA 1 Monoclonal ab


cat# RDI-CATP1/G9    $469.00/vial

Background: The ATP pumps that reside in intracellular organelles are encoded by a family of structurally related enzymes, termed the sarcoplasmic or endoplasmic reticulin CA+ (SERCA) ATPases. The Serca1 gene is exclusively expressed in type II (fast) skeletal muscle.

isotype: mIgG1

clone: VE121G9

package: 100ul of diluted ascites fluid with 0.05% sodium azide added


antigen: produced from a fusion of NS-1 myeloma cells and spleen cells from Balb/c mice immunized with purified rabbit skeletal muscle sarcoplasmic reticulum.

Specificity: recognizes the SerCA1 ATPase in tissues as shown below. It epitope maps to region between amino acids 506 and the C-terminus of rabbbit skeletal muscle ATPase, a region that is expressed in native sarcoplasmic reticulum. This clone also inhibits the crystallization of CA2+ ATPase induced by vanadate. Recognizes Serca1 and Type II (fast) from HUMANS, Canine and rabbit.

Use: -Western blot 1:2500 recognizes approx 110Kda band

         -Immunofluorescence: 1:500 strongly labels the entire type II (fast) myofiber .


Storage: Store at -20 DEG C upon receipt. Avoid frequent freeze thaw cycles. Recommend aliquoting. BEFORE OPENING, suggest centrifuging for 1 minute at 1000 RPM to concentrate material in vial.



References: -C.M. Knudson et al, Journal of Biological Chemistry, 264: 1345-1348, 1988.

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.


It is the responsibility of the user to comply with all local/state andFederal rules in the use of this product. We are not responsible for anypatent infringements that might result with the use of or derivation of this product.


Also available: antibodies to NA K ATPase and CA ATPASE (sera 1 , serca 2 and

serca 3). Please inquire.


RDI Divison of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

Ordering terms

EMAIL:antibodies@fitzgerald-fii.com

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