rev:January 11, 2004
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ANTIBODIES
(anti-Human and others as indicated)
RDI Divison of Fitzgerald Industries Intl offers a wide line of antibodies. Since no one antibody works best for all applications (neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.
Mouse anti-CA ATPase SERCA 1 Monoclonal ab
cat# RDI-CATP1/G9 $469.00/vial
Background: The ATP pumps that reside in intracellular organelles are encoded
by a family of structurally related enzymes, termed the sarcoplasmic or
endoplasmic reticulin CA+ (SERCA) ATPases. The Serca1 gene is exclusively
expressed in type II (fast) skeletal muscle.
isotype: mIgG1
clone: VE121G9
package: 100ul of diluted ascites fluid with 0.05% sodium azide added
antigen: produced from a fusion of NS-1 myeloma cells and spleen cells from
Balb/c mice immunized with purified rabbit skeletal muscle sarcoplasmic
reticulum.
Specificity: recognizes the SerCA1 ATPase in tissues as shown below. It epitope
maps to region between amino acids 506 and the C-terminus of rabbbit skeletal
muscle ATPase, a region that is expressed in native sarcoplasmic reticulum.
This clone also inhibits the crystallization of CA2+ ATPase induced by vanadate.
Recognizes Serca1 and Type II (fast) from HUMANS, Canine and rabbit.
Use: -Western blot 1:2500 recognizes approx 110Kda band
-Immunofluorescence: 1:500 strongly labels
the entire type II (fast) myofiber .
Storage: Store at -20 DEG C upon receipt. Avoid frequent freeze thaw cycles. Recommend aliquoting. BEFORE OPENING, suggest centrifuging for 1 minute at 1000 RPM to concentrate material in vial.
References: -C.M. Knudson et al, Journal of Biological Chemistry, 264: 1345-1348,
1988.
Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.
It is the responsibility of the user to comply with all local/state andFederal rules in the use of this product. We are not responsible for anypatent infringements that might result with the use of or derivation of this product.
Also available: antibodies to NA K ATPase and CA ATPASE (sera 1 , serca 2 and
serca 3). Please inquire.
RDI Divison of Fitzgerald Industries Intl
34 Junction Square Drive
Concord MA 01742-3049
USA
phone (978) 371-6446 or (800) 370-2222
fax (978) 371-2266
EMAIL:antibodies@fitzgerald-fii.com
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