rev:January 11, 2004

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(anti-Human and others as indicated)

RDI Divison of researchd Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.

Mouse anti-CA ATPase SERCA 1 Monoclonal ab

cat# RDI-CATP1/2H11       $469.00/vial

Background: The ATP pumps that reside in intracellular organelles are encoded by a family of structurally related enzymes, termed the sarcoplasmic or endoplasmic reticulin CA+ (SERCA) ATPases. The Serca1 gene is exclusively expressed in type II (fast) skeletal muscle.

isotype: mIgG1

clone: IIH11

package: 100ul of diluted ascites fluid with 0.05% sodium azide added

antigen: produced from a fusion of NS-1 myeloma cells and spleen cells from Balb/c mice immunized with purified rabbit skeletal muscle sarcoplasmic reticulum.

Specificity: recognizes the SerCA1 ATPase in tissues as shown below. It epitope maps to region between amino acids 199 and 505 of rabbbit skeletal muscle ATPase, a   region that is not exposed in native sarcoplasmic reticulum. This clone has been used in western blot and immunofluorescence to detect the Serca1 ATPase. By western blot, the antibody recognizes a single 100kDa band. In immunofluorescence, strongly lables the entire type II (fast) myofiber. Reactive with: Rat, Canine, Mouse, Guinea pig, and rabbit Serca1 and Type II (fast).

Use: -Western blot 1:2500

        -Immunofluorescence: 1:500 .

Storage: Store at -20 DEG C upon receipt. Avoid frequent freeze thaw cycles. Recommend aliquoting. BEFORE OPENING, suggest centrifuging for 1 minute at 1000 RPM to concentrate material in vial.

References: -A.O. Jorgensen et al, Cell Motility and the Cytoskeleton, 9:164-174, 1988.

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.

It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of thisproduct.

Also available: antibodies to NA K ATPase and CA ATPASE (sera 1 , serca 2 and

serca 3).

RDI Divison of researchd Industries Intl

San Jose, 95123 CA Snell ave 658


phone 408-780-0908 or (2975) 584-7093

fax     (975) 584-0210

Ordering terms

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