rev:   November 7, 2003

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ANTIBODIES  

(anti-Human and others as indicated)

RDI Divison of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.


Calsequestrin (see below monoclonal and polyclonal)


Mouse anti-Calsequestrin Monoclonal ab (mouse/canine/rabbit)

cat# RDI-Calseqabm  $469.00/vial

Background: Calsequestrin is a calcium binding protein known to sequester calcium accumulated in the sarcoplasmic reticulum of muscle cells during relaxation and is found discretely localized to the junctional and corbular (terminal cisternae). Calsequestrin functions to localize Ca2+ near the junctional face of the terminal cisternae from which CA2+ can be released into the cytosol via the ryanodine receptor. This protein is highly acidic and has a large capacity and moderate to low affinity for CA2+.

isotype: mIgG2b

clone: VIIID12

package: 100ul of diluted ascites fluid with 0.05% sodium azide added

antigen: produced from a fusion of NS-1 myeloma cells and spleen cells from Balb/c mice immunized with purified rabbit skeletal muscle sarcoplasmic reticulum.

Specificity: calsequestrin in both type I (slow) and type II (fast) skeletal muscle tissues in canine, rabbit and mouse.

Use: -Western blot:1:1000 starting dilution detects the presence of calsequestrin in rabbit, canine and mouse cells. By western blot, recognizes a band at 63Kda representing calsequestrin. Other higher molecular weight bands seen on western blot are calsequestrin- like proteins found in the sarcoplasmic reticulum.

Storage: Store at -20 DEG C upon receipt. Avoid frequent freeze thaw cycles. Recommend aliquoting. BEFORE OPENING, suggest centrifuging for 1 minute at 1000 RPM to concentrate material in vial.

Main REF: C.M. Knudson et al, Journal of Biological Chemistry, 264:1345-1348, 1988.

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.  It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.


Rabbit anti-Calsequestrin (Cardiac/Skeletal)   Human, Ovine, Mouse,Rat+

cat# RDI-CALSEQabR    $469.00/100ul

Package: 100 ul of affinity purified rabbit serum with 1.0 mg/ml BSA and 0.05% sodium azide. Store at -20C. Avoid repeated freeze/thaw cycles.

Derivation: immunizing New Zealand white rabbits with purified canine cardiac calsequestrin.

Use: 1:2500 for western blot

       -This antibody recognizes both cardiac and skeletal muscle calsequestrin from a variety of species. This antibody has been successfully used in Western blots and immunoprecipitation to detect the presence of calsequestrin in canine, human, sheep, rat and mouse cardiac and skeletal muscle. By Western blot, this antibody recognizes a band at ~55 kDa representing cardiac calsequestrin. Additional bands at 97 kDa may be observed and have been reported to be calsequestrin-like proteins.

Backgorund: The sarcoplasmic reticulum (SR) is in part responsible for maintaining the level of intracellular Ca2+ in cardiac and skeletal muscle by storing and releasing Ca2+. Several intralumenal SR Ca2+ binding proteins have been identified, the most prominent of these is calsequestrin. Calsequestrin is a calcium binding protein known to sequester calcium accumulated in the sarcoplasmic reticulum of muscle cells during relaxation and is found discretely localized to the junctional and corbular (terminal cisternae) SR. Calsequestrin functions to localize Ca2+ near the junctional face of the terminal cisternae from which Ca2+ can be released into the cytosol via the ryanodine receptor. This protein is highly acidic and has a large capacity and moderate to low affinity for Ca2+.

Two mammalian isoforms of calequestrin exist, a cardiac and a skeletal muscle isoform, which are the products of different genes and are 63% identical.

Original Reference

L. Mahony and L. Jones. Developmental Changes in Cardiac Sarcoplasmic Reticulum in Sheep. Journal of Biological Chemistry, 261(32): 15257-15265, 1986.

For Research Use Only


RDI Divison of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

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