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ANTIBODIES
(anti-Human and others as indicated)
RDI Division of Fitzgerald Industries Intl offers a wide line of antibodies. Since no one antibody works best for all applications (neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.
Calsequestrin (see below monoclonal and polyclonal)
Mouse anti-Calsequestrin Monoclonal ab (mouse/canine/rabbit)
cat# RDI-Calseqabm $469.00/vial
Background: Calsequestrin is a calcium binding
protein known to sequester calcium accumulated in the sarcoplasmic
reticulum of muscle cells during relaxation and is found discretely localized
to the junctional and corbular
(terminal cisternae). Calsequestrin
functions to localize Ca2+ near the junctional face
of the terminal cisternae from which CA2+ can be
released into the cytosol via the ryanodine
receptor. This protein is highly acidic and has a large capacity and moderate
to low affinity for CA2+.
isotype: mIgG2b
clone: VIIID12
package: 100ul of diluted ascites fluid with 0.05%
sodium azide added
antigen: produced from a fusion of NS-1 myeloma cells and spleen cells from Balb/c mice immunized with purified rabbit skeletal muscle sarcoplasmic reticulum.
Specificity: calsequestrin in both type I (slow) and
type II (fast) skeletal muscle tissues in canine, rabbit and mouse.
Use: -Western blot:1:1000 starting dilution detects
the presence of calsequestrin in rabbit, canine and
mouse cells. By western blot, recognizes a band at 63Kda representing calsequestrin. Other higher molecular weight bands seen on
western blot are calsequestrin- like proteins found
in the sarcoplasmic reticulum.
Storage: Store at -20 DEG C upon receipt. Avoid frequent freeze thaw cycles. Recommend aliquoting. BEFORE OPENING, suggest centrifuging for 1 minute at 1000 RPM to concentrate material in vial.
Main REF: C.M. Knudson et al, Journal of Biological Chemistry, 264:1345-1348, 1988.
Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.
Rabbit anti-Calsequestrin (Cardiac/Skeletal) Human, Ovine, Mouse,Rat+
cat# RDI-CALSEQabR $493.50/100ul
Package: 100 ul of affinity purified rabbit serum
with 1.0 mg/ml BSA and 0.05% sodium azide. Store at
-20C. Avoid repeated freeze/thaw cycles.
Derivation: immunizing
Use: 1:2500 for western blot
-This antibody recognizes both cardiac and skeletal muscle calsequestrin from a variety of species. This antibody has been successfully used in Western blots and immunoprecipitation to detect the presence of calsequestrin in canine, human, sheep, rat and mouse cardiac and skeletal muscle. By Western blot, this antibody recognizes a band at ~55 kDa representing cardiac calsequestrin. Additional bands at 97 kDa may be observed and have been reported to be calsequestrin-like proteins.
Backgorund: The sarcoplasmic reticulum (SR) is in part responsible for maintaining the level of intracellular Ca2+ in cardiac and skeletal muscle by storing and releasing Ca2+. Several intralumenal SR Ca2+ binding proteins have been identified, the most prominent of these is calsequestrin. Calsequestrin is a calcium binding protein known to sequester calcium accumulated in the sarcoplasmic reticulum of muscle cells during relaxation and is found discretely localized to the junctional and corbular (terminal cisternae) SR. Calsequestrin functions to localize Ca2+ near the junctional face of the terminal cisternae from which Ca2+ can be released into the cytosol via the ryanodine receptor. This protein is highly acidic and has a large capacity and moderate to low affinity for Ca2+.
Two mammalian isoforms of calequestrin exist, a cardiac and a skeletal muscle isoform, which are the products of different genes and are 63% identical.
Original Reference
L. Mahony and L. Jones. Developmental Changes in Cardiac Sarcoplasmic Reticulum in Sheep. Journal of Biological Chemistry, 261(32): 15257-15265, 1986.
For Research Use Only
RDI Division of Fitzgerald Industries Intl
phone (978) 371-6446 or (800) 370-2222
fax (978) 371-2266
EMAIL:antibodies@fitzgerald-fii.com
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