rev: 5/16/2008
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ANTIBODIES
(anti-Human and others as indicated)
RDI Division of Fitzgerald Industries Intl offers a wide line of antibodies. Since no one antibody works best for all applications (neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.
Rabbit anti-Calretinin
-Calretinin is a calcium binding protein of the
EF-hand family related to calbindin D-28K and calmodulin. The antibody was
evaluated for specificity and potency by biotin-avidin labelling of cryostat or
vibratome sections of 4% paraformaldehyde fixed brains and b) by
immunoenzymatic labelling of immunoblots.
Catalog#: RDI-CALRETabr
Price: $593.25/vial
LOT# see sheet with shipment
Packaging: 200 microliters lyophilized antiserum (no preservatives)
Species: rabbit
Antigen: recombinant human calretinin
Specificity: human, monkey, rat and mouse tissues -does not cross with
calbindin D-28Kd or other known calcium binding proteins as determined by its
distribution in the brain as well as by immunoblots.
Uses: Immunoblots: 1:1000-1:5000
Immunohistochemistry: 1:5000- 1:10000 with avidin-biotin method.
Optimal dilutions must be determined by the end user for their particular application.
ref: Schwaller B., et al, CELL CALCIUM 14:639-648.
Storage: For long term storage, keep small portions (1-2ul) at -80 DEG C or at
least -20 DEG . For continuous use
keep at 4 DEG C (with 0.01% Na-azide). AVOID FREQUENT FREEZE THAW
CYCLES.
For IN VITRO RESEARCH USE ONLY-NOT FOR USE IN OR ON HUMANS OR
ANIMALS-Notresponsible for any patent infringements with use or derivation of
this or any product.
Additional product of interest: recombinant human calretinin(suitable
for immunoblots, absorption experiments inimmunohistochemistry and RIA). cat#RDI-rCALRET/P 250.00/10ug $1500.00/100ug
mouse anti-calretinin clone 6B3 monoclonal cat#RDI-CALRETabm-6B3 $625.00/200ul
Goat anti-calretinin (polyclonal) cat#RDI-CALRETabG $562.00/200ul
RECOMBINANT HUMAN Calretinin
cat# RDI-rCALRET/P $312.00
$1500.00/100ug
package: 10 micrograms lyophilized from 25ul 10mM Tris-base, 80mM NaCl, 1mM
EDTA, mM B- mercaptoethanol, 0.02% NaN3, pH 7.4).
Source: E Coli
Description: Purified by FPLC
Storage: Freeze in small aliquots (approx 1ug) and keep at
-80 DEG C. Avoid repeated freezing and thawing.
Uses: For absorption: dilute 1ul of the rabbit anti-calretinin, cat#RDI-CALRETabr
in 5ml of the usual buffer for immunohistochemistry (final dilution
1:5000). Add 1ug of the recombinant protein to 1ml of the diluted antibody in
solution and mix well. Incubate for at least 6 hours in the cold. Apply
to tissue sections and incubate for 3 days. Complete the immunohistochemical
reaction as usual (using biotinylated second antibody, ABC-complex, DAB) . -Immunostaining should be strongly reduced or even
completely prevented.
For IN VITRO RESEARCH USE ONLY-NOT FOR USE IN OR ON HUMANS OR ANIMALS-Not responsible for any patent infringements with use or derivation of this or any product.
RDI Division of Fitzgerald Industries Intl
phone (978) 371-6446 or (800) 370-2222
fax (978) 371-2266
EMAIL:antibodies@fitzgerald-fii.com
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