rev:  January 11, 2004

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ANTIBODIES  

(anti-Human and others as indicated)

RDI Divison of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.


Mouse anti-Calcineurin Monoclonal ab

cat# RDI-CALCINabm        $438.00/vial 50ug

       RDI-CALCINabm-1     $750.00/vial 150ug


Background: Calcineurin is a CA2+/calmodulin dependent protein phosphatase. The active enzyme is a heterodimer consisting of a large calmodulin binding catalytic subunit A (61kDa) and a smaller, amino terminus myristylated CA binding subunit B (19kDa). Regions corresponding to the calmodulin-binding site, an autoinhibitory domain, and a putative subunit B binding site have bene identified within the large subunit A. It has recently been demonstrated that calcineurin activity is sensitive to immunosuppressants such as cyclosporin A and tacrolimus (FK506). The study of FK506 mediated inhibition of nitric oxide formation has revealed that nitric oxide synthase (NOS) is a calcineurin substrate. Calcineurin dephosphorylates protein kinase C mediated phosphorylation of NOS. This dephosphorylation enhances catalytic activity of NOS. Calcineurin appears to be an essential mediator of one signal through which antigen stimulation of the T cell antigen receptor (TcR) leads to the activation of T cells.

isotype: mIgG2a


package: 50ug (200ul) or 150ug (600ul) (at 0.25mg/ml in 50% glycerol, PBS + 1.5mM NaN3 & 1mg/ml BSA)


antigen: protein fragment corresponding to amino acids 247-449 of human calcineurin. (in calmodulin binding region)


USE: Suitable for western blot on human, rat, and chick using approx 1 ug/ml ab on total cell of HELA cells. Major band =MW 61Da. -also suitable for inmmunofluoresnce and histochemistry


Storage: Store at -20 DEG C upon receipt. Recommend aliquoting. Avoid frequent warming cycles.


Main REF: -Frantz, B. et al, 1994:EMBO J 13:861

                  -Husi, H. et al 1994 J. Biol.Chem 269:14199

                  -Nichols, R.A. et al. 1994 J. Biol. Chem. 269:23817

                  -Dawson, T.M. eta l. 1993 Proc Natl Acad Sci USA 90:9808


Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.

-copyright by owner


RDI Divison of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

Ordering terms

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