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(anti-Human and others as indicated)
RDI Divison of Fitzgerald Industries Intl offers a wide line of antibodies. Since no one antibody works best for all applications (neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.
CALCIUM CHANNEL ANTIBODIES
LOT# see sheet with shipment
Packaging: 1 milligram IgG fraction in 1ml 0.1M Tris-Hcl, pH 7.5.
Species: rabbit IgG purified by Protein G Sepharose chromatography.
Antigen: each of the above antibodies were made individually with a synthetic peptide, designated A, B and C (respectively).
Specificity: human and rat. recognizes the alpha-1 subunit of the type N Calcium channel by immunoprecipitation.
Uses: Optimal dilutions must be determined by the end user for their particular application.
ref: -Dubel, S.J. et al, Proc. Natl. Acad. Sci. (USA) 89, 5058-5062 (1992)
-Starr, T.V.B. et al, Proc. Natl. Acad. Sci (USA) 88,
Storage: Store at -20 DEG C. AVOID FREQUENT FREEZE THAW CYCLES.
cat# RDI-CACHANA2abm $500.00/vial
LOT# see sheet with shipment
Packaging: 200 microliters in 100mM Hepes, 20nM Na-Citrate pH 7.4 with 0.05% NaN3.
Species: mouse IgG2b
Antigen: A2 subunit Ca Channel protein purified from the T- tubular membranes of rabbit skeletal muscles.
Specificity: specific for the calcium channel of skeletal muscles in: fish, frog, chicken, pig, sheep, bovine, guinea pig, mouse rat and human. It does cross react with the calcium channel of other tissue including brain, cardiac muscle, uterus and tracea.
-Immunoblots: 1:5000-1:10000 (blots a polypeptide of 135 Kda corresponding to the Alpha 2 subunit of the Ca-channel).
Immunohistochemistry: 1:5000- 1:10000 with avidin biotin method
Optimal dilutions must be determined by the end user for their particular application.
ref: -Smith R.G. et al (1992), Serum Antibodies to L-
type calcium channels in patients with amyotrophic
lateral sclerosis. New Engl. J. Med. 327:1721-
-Hullin R. et al (1993) Tissue specific expression
of calcium-channels. Trends Cardiovasc Med 3:48- 53.
-Schneider et al (1992) Purification and structure
of L-type Calcium channels In:Methods in Molecular
Biology., Vol 13:Protocols in Molecular
Neurobiology. Ed. A. Longstaff and P. Revest.
Humana Press, Totowa NJ, pp. 273-286.
Storage: For long term storage, keep small portions (1-2ul) at -80 DEG C
or at least -20 DEG C. For continuous use keep at 4 DEG C (with 0.01% Na-azide).
AVOID FREQUENT REEZE THAW CYCLES.
Additional product of interest: partially purified Ca channel.
cat#RDI-CACHAN/P 250.00/10 micrograms Blotting control
34 Junction Square Drive
Concord MA 01742-3049
phone (978) 371-6446 or (800) 370-2222
fax (978) 371-2266
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