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(anti-Human and others as indicated)

RDI Divison of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.


Rabbit anti-Human Calcium Channel (TYPE N)

Background: Anti-human N-type Ca channel alpha subunit IgG fraction antibodies were raised in rabbits agianst three peptides,designated A, B and C. These peptides were originally recognized in rat brain Ca channels (ref 1).

cat# RDI-CACHAN9Aabr    $438.00

       RDI-CACHAN9Babr    $438.00

       RDI-CACHAN9Cabr    $438.00

LOT# see sheet with shipment

Packaging: 1 milligram IgG fraction in 1ml 0.1M Tris-Hcl, pH 7.5.

Species: rabbit IgG purified by Protein G Sepharose chromatography.

Antigen: each of the above antibodies were made individually with a synthetic peptide, designated A, B and C (respectively).

Specificity: human and rat. recognizes the alpha-1 subunit of the type N Calcium channel by immunoprecipitation.

Uses: Optimal dilutions must be determined by the end user for their particular application.

ref: -Dubel, S.J. et al, Proc. Natl. Acad. Sci. (USA) 89, 5058-5062 (1992)

      -Starr, T.V.B. et al, Proc. Natl. Acad. Sci (USA) 88, 5621-5625 (1991)


Mouse anti-Calcium Channel ALPHA 2-Subunit Monoclonal ab

cat# RDI-CACHANA2abm     $500.00/vial

LOT# see sheet with shipment

Packaging: 200 microliters in 100mM Hepes, 20nM Na-Citrate pH 7.4 with 0.05% NaN3.

Species: mouse IgG2b

Antigen: A2 subunit Ca Channel protein purified from the T- tubular membranes of rabbit skeletal muscles.

Specificity: specific for the calcium channel of skeletal muscles in: fish, frog, chicken, pig, sheep, bovine, guinea pig, mouse rat and human. It does cross react with the calcium channel of other tissue including brain, cardiac muscle, uterus and tracea.


-Immunoblots: 1:5000-1:10000 (blots a polypeptide of 135 Kda corresponding to the Alpha 2 subunit of the Ca-channel).

Immunohistochemistry: 1:5000- 1:10000 with avidin biotin method

Optimal dilutions must be determined by the end user for their particular application.

ref: -Smith R.G. et al (1992), Serum Antibodies to L-

       type calcium channels in patients with amyotrophic

       lateral sclerosis. New Engl. J. Med. 327:1721- 1728.

     -Hullin R. et al (1993) Tissue specific expression

      of calcium-channels. Trends Cardiovasc Med 3:48- 53.

     -Schneider et al (1992) Purification and structure

       of L-type Calcium channels In:Methods in Molecular

       Biology., Vol 13:Protocols in Molecular

       Neurobiology. Ed. A. Longstaff and P. Revest.

       Humana Press, Totowa NJ, pp. 273-286.

Storage: For long term storage, keep small portions (1-2ul) at -80 DEG C or at least -20 DEG C. For continuous use keep at 4 DEG C (with 0.01% Na-azide). AVOID FREQUENT REEZE THAW CYCLES.

Additional product of interest: partially purified Ca channel.

cat#RDI-CACHAN/P 250.00/10 micrograms Blotting control

RDI Divison of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049


phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

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