rev:5/16/2008
HOME (index page)
Return (ion transport data page)
ANTIBODIES
(anti-Human and others as indicated)
RDI Division of Fitzgerald Industries Intl offers a wide line of antibodies. Since no one antibody works best for all applications (neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.
Mouse anti-Na+/K+ alpha 1 subunit Monoclonal ab
cat# RDI-atpa1abm $517.00/vial
Background: The NA+/K+ Atpase is an integral membrane
enzyme found in all cells of higher organisms and is responsible for the
ATP-dependent transport of NA and K across the cell membrane. The enzyme
consists of a large multipass transmembrane
catalytic subunit (alpha subunit) and an associated smaller glycoprotein (Beta
subunit). Studies indicate that there are three isoforms
of the alpha subunit (A1, a2, & A3) and two isoforms
ofthe Beta subunit (B1 & 2) which are encoded by
two multigene families. The alpha 3 subunit has been
found in neuronal and to a lesser extent skeletal and smooth muscle.
isotype: mIgG1
clone: 9A-5
package: 100ul of diluted ascites fluid with 0.05%
NaN3
antigen: produced from a fusion of NS-1/1-Ag-4 mouse myeloma cells and spleen
cells from balb/c mice immunized with purified rat kideny Na+ K+ Atpase.
Specificity: alpha 1 subunit of NA+/K+ ATPase -in
human, canine ,rat and avaian tissues. -binds to
region within an intracellular region at or near Asp-369 of the alpha subunit.
Use: -inhibit activity of enzyme
-affinity purification of the A subunit of the NA K ATPase
-immunoprecipitation
-Immunohistochemistry: 1:1000 stains consistent with the
plasma membrane localization of NA+/K+ ATPase.
-affinity purification of the alpha subunit.
-Elisa
ref: D.B. Schenk et al, Proc. Natl. Acad. Sci, 80:5281-5285, 1983
D.B. Schenk, et al, J. Biological Chem 259(23) 14941-14951, 1984.
J.J. Hubert et al, Biochemistry, 25:4156-4163, 1986.
Storage: Store at -20 DEG C upon receipt. Avoid frequent freeze thaw cycles.
Recommend aliquoting. BEFORE OPENING, suggest
centrifuging for 1 minute at 1000 RPM to concentrate material in vial.
Precautions: For In vitro research Use Only. Not for use in or on humans or
animals or for diagnostics. It is the responsibility of the user to comply with
all local/state and Federal rules in the use of this product. We are not
responsible for any patent infringements that might result with the use of or
derivation of this product.
RDI Division of Fitzgerald Industries Intl
phone (978) 371-6446 or (800) 370-2222
fax (978) 371-2266
EMAIL:antibodies@fitzgerald-fii.com
RETURN (ion transport data page)