rev:January 11, 2004

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ANTIBODIES  

(anti-Human and others as indicated)

RDI Divison of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.


Mouse anti-Na+/K+ alpha 1 subunit Monoclonal ab

cat# RDI-atpa1abm     $469.00/vial

Background: The NA+/K+ Atpase is an integral membrane enzyme found in all cells of higher organisms and is responsible for the ATP-dependent transport of NA and K across the cell membrane. The enzyme consists of a large multipass transmembrane catalytic subunit (alpha subunit) and an associated smaller glycoprotein (Beta subunit). Studies indicate that there are three isoforms of the alpha subunit (A1, a2, & A3) and two isoforms ofthe Beta subunit (B1 & 2) which are encoded by two multigene families. The alpha 3 subunit has been found in neuronal and to a lesser extent skeletal and smooth muscle.


isotype: mIgG1

clone: 9A-5

package: 100ul of diluted ascites fluid with 0.05% NaN3

antigen: produced from a fusion of NS-1/1-Ag-4 mouse myeloma cells and spleen cells from balb/c mice immunized with purified rat kideny Na+ K+ Atpase.

Specificity: alpha 1 subunit of NA+/K+ ATPase -in human, canine ,rat and avaian tissues. -binds to region within an intracellular region at or near Asp-369 of the alpha subunit.


Use: -inhibit activity of enzyme

        -affinity purification of the A subunit of the NA K ATPase

         -immunoprecipitation

         -Immunohistochemistry: 1:1000 stains consistent with the

           plasma membrane localization of NA+/K+ ATPase.

         -affinity purification of the alpha subunit.

         -Elisa

ref: D.B. Schenk et al, Proc. Natl. Acad. Sci, 80:5281-5285, 1983

     D.B. Schenk, et al, J. Biological Chem 259(23) 14941-14951, 1984.

      J.J. Hubert et al, Biochemistry, 25:4156-4163, 1986.



Storage: Store at -20 DEG C upon receipt. Avoid frequent freeze thaw cycles. Recommend aliquoting. BEFORE OPENING, suggest centrifuging for 1 minute at 1000 RPM to concentrate material in vial.


Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.


RDI Divison of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

Ordering terms

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