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Androgen RECEPTOR   ANTIBODIES  

(anti-Human and others as indicated)

RDI Divison of researchd Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.

cat# RDI-A1RECPTabm      $406.00/100ug

       RDI-A1RECPTabm-1   $562.00/200ug

Presentation: Protein G purified antibody in 10mM PBS with 0.2% BSA and 15mM   NaN3. (Bulk available without BSA/Azide upon request)   concentration 200ug/ml

Immunogen: synthetic peptide , aa 299-315 of human AR

           sequence: S T E D T A E Y S P F K G G Y T K

Isotype: mIgG1

Clone: AR 441

Reactivity: human (does not react with mouse)

Uses: -Western blot: 1ug/ml

          -histochemistry (frozen and paraffin sections)   approx 1:100 , 60 minutes RT

        (paraffin * (Staining of formalin-fixed tissues REQUIRES boiling   tissue sections in 1mM EDTA, pH 8.0 for 10-20 min followed by   cooling at RT for 20 min.)

Background: Recognizes a protein of 110kDa which is identified as androgen receptor (AR). It reacts with full length, and the newly described A form of the receptor. It does not cross react with estrogen, progesterone, or glucocorticoid receptors. The expression of AR is reportedly inversely correlated with histologic grade i.e. well differentiated prostate tumors show higher expression than the poorly differentiated tumors. In prostate cancer, AR has been proposed as a marker of   hormone-responsiveness and thus it may be useful in identifying patients likely to benefit from anti- androgen

Storage: 2-8 deg c

References:

Mol Endocrinol, 2:1265-75, 1988.

Science, 240:889-95, 1988.

Cell, 56:335-44, 1989.

Crit Rev Biochem Mol Biol, 24:101-17, 1989.

FASEB J, 5:3092-9, 1991.

FASEB J, 5:2243-9, 1991.

Clin Chem, 39(2):346-52, 1993.

For Research Use Only-Not for use in diagnostics

cat#RDI-ANDRECPTabr   $438.00/100ug vial

Steroid receptors are ligand-dependent intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate hormone. Androgens induce development in male-specific organs, such as the prostate and seminal vesicle, and later in life they control the functional activity of target tissues. Androgens exert their effects by binding to the androgen receptor (AR) which has been found in a variety of tissues, including reproductive organ tissue, central nervous system tissue, and skeletal muscle.

This antibody is known to react with numerous species including primates, bovine, sheep, pigs, rodents, birds, frogs and fish. This product does not cross-react with estrogen, progesterone, or glucocorticoid receptors. Competition studies using the antigenic peptide at a 10 molar excess, completely blocked nuclear staining whereas other non-related peptides had no effect on immunohistochemical staining.

The antibody has been used to detect AR by various procedures, including immunocytochemistry, immunohistochemistry and Western blot.  By Western blot, this product detects a ~110 kDa band representing AR, faint bands at 70 and 75 kDa are also seen in pre-immune sera.  Immunohistochemical studies of both frozen and formalin-fixed, paraffin-embedded (FFPE) sections show intense nuclear staining consistent with nuclear localization of AR. With FFPE sections, antigen retrieval methods using microwave fixation must be utilized. New Zealand White Rabbits were immunized with the synthetic peptide corresponding to the N-terminal 21 amino acids of rat and human AR.  An additional cysteine residue was added at the C-terminal end for conjugation to KLH. Specific antibody against the antigenic peptide was epitope purified by affinity chromatography.

Peptide sequence: M(1) E V Q L G L G R V Y P R P P S K T Y R G(21) C

Uses & Suggested Working Concentrations

            western blot: 4ug/ml

             immunohistochemistry: 4ug/ml

Optimal titer must be determined by the end user for their particular application.

Packaging: 100 ug of purified rabbit antibody in 0.1ml (1.0mg/ml) PBS buffer with 1mg/mL BSA and 0.05% sodium azide. Keep in concentrated form until use. Store at 4-8 DEG C DO NOT FREEZE

General related Reference:

G. S. Prins, L. Birch, G. L. Greene. Androgen Receptor Localization in Different Cell Types of the Adult Rat Prostate. Endocrinology, 129(6): 3187-3199, 1991.

For In vitro Research Use Only-Not For Use in Diagnostics.

San Jose, 95123 CA Snell ave 658

USA

or (800) 370-2222

EMAIL:margaret@cellular-research.com

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