rev: May 14, 2003
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Anti-RAT
CD CLUSTERED
ANTIBODIES
RDI Division of Fitzgerald Industries Intl offers a wide line of antibodies. Since no one antibody works best for all applications (flow cytometry, neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below. All products are for in vitro research use only-not for use in or on humans or animals-not for use in diagnostics. Price/availability/specifications subject to change without notice.
Anti-RAT CD90
Antibodies (2 clones)
DATA SHEET: mouse anti-RAT/Mouse CD90 (THY-1.1)
Catalog#
RDI-RTCD90-OX7 $406.00/vial
also available: FITC labeled cat#RDI-RTCD90-OX7FT $562.00/0.5mg
Biotin labeled cat#RDI-RTCD90-OX7BT $562.00/0.5mg
PE labeled cat#RDI-RTCD90-OX7PE
$438.00/0.2mg
LOT# see batch sheet
Package Size: 500ug purified with 0.09% NaN3
Species: mouse balb/c IgG1k
CLONE: OX-7
Activity: This monoclonal reacts with mouse and rat CD90 (Thy-1.1 antigen).
In the rat, THY-1.1 is expressed by hematopoietic stem cells, immature B
lymphocytes in the bone marrow and peripheral lymphoid organs, thymocytes
and recent emigrants (a subset of CD45RC- peripheral T cells). In the mouse,
THY-1.1 is found on thymocytes, all peripheral thymoctes, some intraepithelial
T lymphocytes (IEL, DEC) and hematopoietic stem cells, but not B cells, in
strains expressing the THY1.1 allotype (eg, AKR/J, PL). Does not cross with
THY1-2.
Application: Indirect immunofluorescence cell surface staining.
-histochemistry (acetone fixed frozen and paraffin embedded sections 1-20ug/ml)
Storage: Store at 4 DEG C for up to 30 days. Prolonged storage at -20 DEG
C. Avoid repeated freeze thaw cycles.
Precautions: For In vitro research Use Only. Not for use in or on humans
or animals or for diagnostics.
References: Biochem. J. 187:1-20
Biochem. J. 195:15-30
J. Immunol. 150:1670-1679
It is the responsibility of the user to comply with all local/state and Federal
rules in the use of this product. We are not responsible for any patent
infringements that might result with the use of or derivation of this product.
Catalog#: RDI-RTCD90-HIS5 $438.00/vial $375.00/vial 5+ Bulk
quotes on request
also FITC labeled cat#RDI-RTCD90-HIFT $625.00/0.5mg
Package Size: 500ug purified in 1.0ml (0.5mg/ml) 10mM phosphate buffer and
0.09% sodium azide.
Species: mouse balb/c IgG1k
CLONE: HIS51
Immunogen: Mouse (BALB/c) IgG2ak
Purification: affinity purified from TCS
Background: The HIS51 antibody reacts with the rat Thy-1 antigen (CD90)1
expressed by hematopoietic stem cells, early myeloid and erythroid cells,immature
B lymphocytes in the bone marrow and peripheral lymphoid organs, thymocytes,
recent thymic emigrants (a subset of CD45RC - peripheral T cells), neurons,
glomerular mesangial cells, endothelium at inflammatory sites, mast cells,
and bone marrow-derived dendritic cells.9 Rat dendritic epidermal T cells
(DEC) are Thy-1 negative. CD90 is a GPI-anchored membrane glycoprotein of
the Ig superfamily which is involved in signal transduction. In addition,
there is evidence in the mouse that CD90 mediates adhesion of thymocytes
to thymic stroma. Clone HIS51 antibody cross-reacts with the mouse Thy-1.1
alloantigen of the AKR/J and PL strains, but not Thy-1.2 found on most strains.
In the mouse, CD90 is found on thymocytes, most peripheral T lymphocytes,
some intraepithelial T lymphocytes (IEL, DEC), hematopoietic stem cells,
and neurons, but not B lymphocytes.
Use: -FACS staining (1 µg/1 million cells) with flow cytometric
-immunohistochemical staining (IHC) of frozen, formalin-, and zinc-fixed paraffin-embedded sections
-and in vivo induction of glomerulonephritis in the rat (in vivo must remove azide and sterile filter).
REFERENCES
1. Hermans, M.H.A. and D. Opstelten. 1991. In situ visualization of hemotopoietic cell subsets and stromal elements in rat and mouse bone marrow by immunostaining of frozen sections. J. Histochem. Cytochem. 39: 1627 - 1634.
2. Campbell, D.G., J. Gagnon, K.B.M. Reid, and A.F. Williams. 1981. Rat brain Thy-1 glycoprotein. The amino acid sequence, disulphide bonds and an unusual hydrophobic region. Biochem. J. 195: 15 - 30.
3. Kroese, F.G.M., N.K. de Boer, T. de Boer, P. Nieuwenhuis, A.B. Kantor, and G.J. Deenen. 1995. Identification and kinetics of two recently bone marrow-derived B cell populations in peripheral lymphoid tissues. Cell. Immunol. 162: 185 - 193.
4. Hosseinzadeh, H., and I. Goldschneider. 1993. Recent thymic emigrants in the rat express a unique antigenic phenotype and undergo post-thymic maturation in peripheral lymphoid tissues. J. Immunol. 150: 1670 - 1679.
5. Groen, H., F.A. Klatter, A.S. van Petersen, J.M. Pater, P. Nieuwenhuis, and J. Kampinga. 1993. Composition of rat CD4 + resting memory T-cell pool is influenced by major histocompatibility complex. Transplant. Proc. 25: 2782 - 2783.
6. Paul, L.C., H.G. Rennke, E.L. Milford, and C.B. Carpenter. 1984. Thy-1.1 in glomeruli of rat kidneys. Kidney Int. 25: 771 - 777.
7. Ishizu, A., H. Ishikura, Y. Nakamaru, E. Takeuchi, C. Kimura, T. Koike, and T. Yoshiki. 1995. Thy-1 induced on rat endothelium regulates vascular permeability at sites of inflammation. Int. Immunol. 7: 1939 - 1947.
8. Dráberová, L’., M. Amoui, and P. Dráber. 1996. Thy-1-mediated activation of rat mast cells: the role of Thy-1 membrane microdomains. Immunology 87: 141 - 148.
9. Chen-Woan, M., C.P. Delaney, V. Fournier, Y. Wakizaka, N. Murase, J. Fung, T.E. Starzl, and A.J. Demetris. 1996. In vitro characterization of rat bone marrow-derived dendritic cells and their precursors. J. Leukoc. Biol. 59: 196 207.
10. Elbe, A., O. Kilgus, T. Hünig, and G. Stingl. 1993. T-cell receptor diversity in dendritic epidermal T cells in the rat. J. Invest. Dermatol. 102: 74 - 79.
11. Nakashima, I., M.-Y. Pu, M. Hamaguchi, T. Iwamoto, S.M.J. Rahman, Y.-H. Zhang, M. Kato, K. Ohkusu, Y. Katano, T. Yoshida, Y. Koga, K.-I. Isobe, and F. Nagase. 1993. Pathway of signal delivery to murine thymocytes triggered by co-crosslinking CD3 and Thy-1 for cellular DNA fragmentation and growth inhibition. J. Immunol. 151: 3511 - 3520.
12. He, H.-T., P. Naquet, D. Caillol, and M. Pierres. 1991. Thy-1 supports adhesion of mouse thymocytes to thymic epithelial cells through a Ca 2+ -independent mechanism. J. Exp. Med. 173: 515 - 518.
For In vitro Research Use Only-Not for use in or onhumans or animals We are not be responsible for violations or patent infringements which may occur with the use of or derivation of our products.
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phone (800) 370-2222
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EMAIL:antibodies@fitzgerald-fii.com