rev: August 6, 2003
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Anti-RAT
CD CLUSTERED
ANTIBODIES
RDI Division of Fitzgerald Industries Intl offers a wide line of antibodies. Since no one antibody works best for all applications (flow cytometry, neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below. All products are for in vitro research use only-not for use in or on humans or animals-not for use in diagnostics. Price/availability/specifications subject to change without notice.
Anti-RAT CD63
Antibodies
DATA SHEET: mouse anti-Rat CD 63 clone (AD-1)
Catalog#: RDI-RTCD63-AD1 $281.00/vial
Package Size: 100ug in 0.2ml (0.5mg/ml) buffer with 0.09% sodium
azide
Species: mouse IgG1k
Immunogen: Rat basophilic leukemia cell line (RBL-2H3) fused with cells of
NSO myeloma cell line.
CLONE: AD-1
Purity: purified from TCS by affinity chromatography
Activity: The AD1 antibody reacts with a 50 60 kDa protein that is a homolog
(87%) of human CD63 (melanoma-associated antigen ME491). CD63, a highly
glycosylated type-III lysosomal glycoprotein, is a member of the tetraspan
transmembrane superfamily (TM4SF) that also includes CD9, CD37, and CD53.
In the rat, CD63 is expressed on mast cells and platelets.CD63 is also found
on rat embryonic fibroblasts and hepatic cell, along with cultured cell lines
of diverse origin (e.g., liver, lung, and heart). Expression is upregulated
with culture. CD63 is expressed in close proximity to the Fc RI on rat mast
cells and has been shown to partially inhibit IgE-mediated histamine release.
The function of CD63 has not been fully characterized.
Application: -Indirect fluorescence (approx 1ug/million cells) recommend amplifed detection, anti- mouse IgG:Biotin followed by Streptavidin:PE
-acetone fixed frozen sections
-immunoprecipitation,
-partial inhibition of IgE-mediated histamine release and The expression of CD63 on rat
CD54 +
-titers must be determined for each application
Storage: Store at 4 DEG C.
Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.
References: 1. Kitani, S. et al. A cell surface glycoprotein of rat basophilic leukemia cells close to the high affinity IgE receptor (Fc RI). Similarity to human melanoma differentiation antigen ME491. J. Biol. Chem. 266: 1903 1909.
2. Nishikata, H. et al 1992. The rat mast cell antigen AD1 (homologue to human CD63 or melanoma antigen ME491) is expressed in other cells in culture. J. Immunol. 149: 862 870.
3. Barclay, A.N. et al, 1997. The Leucocyte Antigen Facts Book, 2 nd Edition. Academic Press, San Diego, CA, pp. 304 305. 4. Boros, M. et al 1995. Response of mucosal mast cells to intestinal ischemia-reperfusion injury in the rat. Shock 3: 125 - 131.
Conditions: Not be responsible for any patent infringements which use or derivation of any products.
FOR Research Use Only-not for use in or on humans or animals-not for use in diagnostics
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RDI Division of Fitzgerald Industries Intl
34 Junction Square Drive
Concord MA 01742-3049
USA
phone (978) 371-6446 or (800) 370-2222
fax (978) 371-2266
EMAIL:antibodies@fitzgerald-fii.com