rev: August 23, 2002

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Anti-RAT
CD CLUSTERED ANTIBODIES  

RDI Division of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (flow cytometry, neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below. All products are for in vitro research use only-not for use in or on humans or animals-not for use in diagnostics. Price/availability/specifications subject to change without notice.

Anti-RAT  CD61  Antibodies

DATA SHEET:mouse anti rat CD61 (Integrin Beta 3 chain), monoclonal

Catalog#: RDI-RTCD61-F11  Price: $562/1pkg (azide free/sterile)

-also available: FITC labeled cat#RDI-rtCD61-FT $562.00/0.5mg

-non labeled with azide cat#RDI-RTCD61abm $438.00/0.5mg

Package Size: 0.5mg in 0.5ml (1mg/ml)10mM Phosphate   buffer,pH 7.2 with 150mM NACL (sterile filtered).no preservative) Endotoxin level is less than 0.01ng/ug of protein.

Clone: F11

Ig Isotype: mouse IgG1k

Immunogen: Sprague-Dawley rat bone cell suspensions

Reactivity: Reacts with the intergin beta 3 subunit found on endothelial cells, and at high levels on osteoclasts, as the heterodimer AvB3 integrin (vitronectin receptor) and on megakaryocytes and platelets primarily in association with alpha2B integrin subunit (CD41). Clone F11 has been reported to block the in vitro attachement of osteoclasts to several ligands. Weak reactivity with human osteoclast, magakaryocytes and platelets has also been observed.

USE: flow cytometry(<1ug/1M cells)

       -histochemistry (acetone fixed frozen sections) and paraffin sections (recommend zinc fixed paraffin

       sections, not formalin fixed)

       immunoprecipitation

       function blocking

ref: -Helfrich, M.H. et al J. Bone Miner. Res 7:335-343

       -Helfrich, M.H. eta l, J. Bone Miner. Res 7:335-343.

Storage: Store at 4 D under asceptic conditions, or Aliquot. Avoid frequent freeze thaw cycles. If not used for function blocking, can add 0.1% NaN3 as preservative.

Precautions: For In vitro research Use Only. Not for usein or on humans or for diagnostics.

Fixation in Zinc-Fixative

1. Prepare 0.1M Tris buffer, pH 7.4 (IL) Tris Base 12.1 g (THAM, TRIZMA) Deionized water 900 mls 1.o N HCL 81.5 mls

2. Prepare Zinc-Fixative as follows:

0.1M Tris buffer, pH 7.4 recipe above (1000 ml)

0.5g Calcium Acetate

5.0g Zinc Acetate

5.0g Zinc Chloride

Mix to dissolve. The final pH will be approximately 6.5-7.0. Do not readjust the pH, as this will cause the zinc to come out of solution.

3. Store Zinc Fixative at room temperature.

4. Fix tissues in Zinc Fixative by placing freshly dis-sected tissue pieces (no larger than 5 mm in size) in Zinc Fixative and allowing to sit 6 hours at room temp-erature. After fixation, dehydrate tissues for one houreach in 70% ethanol, 90% ethanol, 95% ethanol, and 100% ethanol (2 changes) at room temperature. Tissues are  then cleared in 2 changes of xylene for 50 minutes each at room temperature, followed by paraffin embedding.

Note: The use of an alkaline phosphatase detection system may be needed as this fixation method has been reported to be non-compatible with blocking of endogenous peroxidase activity.

REFERENCES

Nitta,H. etal. 1997. Improved InSitu Immunodetection of Leukocytes on Paraffin-Embedded Mouse Spleen. Cell Vision

4: 73-80

Beckstead JH: A simple technique for preservation of fixation-sensitive antigens in paraffin-embedded tissues. The Journal of Histochemistry and Cytochemistry 1994;42:1127

Beckstead JH: Letter to the editor. Journal of Histochemistry and Cytochemistry 1995;43:345 (This is the actual formulation of the fixative).

Catalog#: RDI-MCD61-2C9 $500.00

Package Size: 0.5mg purified ab in 1ml (0.5mg/ml) buffer with 0.09% sodium azide

Clone: 2C9.G2 (subclone of HMB3-1)

Ig Isotype: Armenian Hamster IgG, group 1K

Purification: Purified from aTCS by affinity chromatography

Immunogen: vitronectin receptor protein from the mouse T-cell hybridoma 2B4

Reactivity: Reacts with the integrin B3 subunit, which associates with the Av chain, to form the vitronectin receptor (CD51/CD61) found on endothelial cells and osteoclasts, and with the A2b chain on platelets and magakaryocytes. Cross reactivity to rat mast cells and platelets has been observed by flow cytometry. Mab 2C9.G2 has been demonstrated to block binding of rat ant mouse ells to fibronectin.

USE: flow cytometry(<1ug/1M cells)

         histochemistry (acetone fixed frozen sections)

      -ligand blocking (azide free)

      -initiation of AvB3 intergin signaling

ref: -Internat. Immunol. 7:251-258 (1995)

       -J Biol Chem 20:1152-11531 (1995)

      -Science 287:860-864 (2000)

Storage: Store at 4-8 DEG C.

Precautions: For In vitro research Use Only. Sodium azide may form explosive compounds in presence of heavy metals or under acidic conditions. Flush drains with copious amounts of water to prevent buildup of explosive compounds.

-also available biotin labeled cat#RDI-MCD61-2C9BT $562.00/0.5mg

-azide free formulation:cat#RDI-MCD61-2CXP $625.00/0.5mg

FITC onjugated cat#RDI-MCD61-2CFT $594.00/0.5mg

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RDI Division of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com