rev:   March 27, 2003

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Anti-RAT
CD CLUSTERED ANTIBODIES  

RDI Divison of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (flow cytometry, neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below. All products are for in vitro research use only-not for use in or on humans or animals-not for use in diagnostics. Price/availability/specifications subject to change without notice.


Anti-RAT  CD45RA  Antibodies


DATA SHEET:mouse anti -RAT CD45RA clone OX-33

cat# RDI-RTCD45RA-33           $438.00/0.5mg

       RDI-RTCD45RA-33FT       $625.00/0.5mg FITC conj

      RDI-RTCD45RA-33PE        $625.00/200ug PE conj

Presentation: 0.5mg purified antibody in 1ml (0.5mg/ml( buffer with 0.09% sodium azide

Clone: OX-33

Isotype: Mouse (BALB/c) IgG1k

Immunogen: Leukocyte common antigen purified from rat splenocytes

Purification: The antibody was purified from tissue culture supernatant by affinity chromatography.

USE: -flow cytometry approx 1ug/million cells Note: It has observed that the staining intensity of OX-33 mAb is reduced after fixation of stained leukocytes (3 hours with 1% formaldehyde). Therefore, one should not fix the stained cells prior to flow cytometry.Freshly-isolated leukocytes and cell lines may wait for analysis in wash buffer at 4°C, without fixation, for up to 18 hours post-staining without loss of viability. Activated lymphocytes may lose viability rapidly, and data should be collected within 5 hours post-staining.

-histochemical staining (IHC) of acetone-fixed frozen and zinc-fixed paraffin-embedded sections. (not recommned for formalin fixed paraffin) titrate 5- 10ug/ml

SPECIFICITY: The OX-33 antibody reacts with a high-molecular-weight form of CD45 found only on B lymphocytes.1 CD45 is a member of the Protein Tyrosine Phosphatase (PTP) family: Its intracellular (COOH-terminal) region contains two PTP catalytic domains, and the extracellular region is highly variable due to alternative splicing of exons 4, 5, and 6 (designated A, B, and C, respectively), plus differing levels of glycosylation.2 The CD45 isoforms detected in the rat are cell type-, maturation-, and activation state-specific.2 The CD45 isoforms play complex roles in T-cell and B-cell antigen receptor signal transduction.2

STORAGE: undiluted at 4°C. Avoid frequent warming cycles

Caution: Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer.

REFERENCES

1. Woollett, G.R., A.N. Barclay, M. Puklavec, and A.F. Williams. 1985.Molecular and antigenic heterogeneity of the rat leukocyte-common antigen from thymocytes and T and B lymphocytes. Eur. J. Immunol. 15: 168 - 173.

2. Johnson, P., A. Maiti, and D.H.W. Ng. 1997. CD45: A family of leukocyte-specific cell surface glycoproteins. In Weir’s Handbook of Experimental Immunology, Vol. 2. L.A. Herzenberg, D.M. Weir, L.A. Herzenberg, and C. Blackwell, eds. Blackwell Science, Cambridge, MA, pp. 62.1 - 62.16.

For Research Use Only


Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. Sodium azide may form explosive compounds in presence of heavy metals or under acidic conditions. Flush drains with copious amounts of water to prevent buildup of explosive compounds.


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RDI Divison of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

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