Mouse anti-RAT CD45R antibodies from RDI Division of Fitzgerald Industries Intl

rev: March 27, 2003

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Anti-RAT
CD CLUSTERED ANTIBODIES

RDI Division of Fitzgerald Industries Intl offers a wide line of antibodies. Since no one antibody works best for all applications (flow cytometry, neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below. All products are for in vitro research use only-not for use in or on humans or animals-not for use in diagnostics. Price/availability/specifications subject to change without notice.

Anti-RAT CD45R Antibodies

DATA SHEET:mouse anti -RAT CD45R clone HIS24

cat# RDI-RTCD45R-HI24 $500.00/0.5mg

Presentation: 0.5mg in 1ml (0.5mg/ml) in buffer with 0.09% sodium azide

available FITC cat#RDI-RTCD45R-HIFT $625.00/0.5mg

PE cat#RDI-RTCD45R-HIPE $625.00/200ug

clone: HIS24

Isotype: mIgG2bk

Immunogen: Low-density cells from AO/G rat Peyer’s patches

Prep: The antibody was purified from tissue culture supernatant by affinity chromatography.

Specifcity: The HIS24 antibody reacts with a developmentally regulated form of CD45 found on most B lymphocytes, including developing B cells in the bone marrow and peripheral B cells, but not plasma cells. The level of expression of this CD45R antigen appears to be an indicator of both maturational stages in the B-cell lineage and of functionally distinct B-cell subsets. The HIS24 mAb, in combination with other markers such as TdT and Ig expression, is effective in the identification of B-cell progenitors. CD45 is a member of the Protein Tyrosine Phosphatase (PTP) family: Its intracellular (COOH-terminal) region contains two PTP catalytic domains, and the extracellular region is highly variable due to alternative splicing of exons 4, 5, and 6 (designated A, B, and C, respectively), plus differing levels of glycosylation. The CD45 isoforms detected in the rat are cell type-, maturation-, and activation state-specific. The CD45 isoforms play complex roles in T-cell and B-cell antigen receptor signal transduction.

Use: -immunofluorescent staining (1 µg/million cells)by FACS

-immunohistochemical staining 5-10ug/ml (IHC) of acetone-fixed frozen,zinc-fixed

paraffin-embedded, and formalin-fixed paraffin-embedded sections

-western blot analysis.

Optimum titers must be assesed for each use

Storage: Store 4 DEG C

REFERENCES

1. Kroese, F.G.M., D. Opstelten, A.S. Wubbena, G.J. Deenen, J. Aten, E.H. Schwander, L. de Leij, and P. Nieuwenhuis. 1986. Monoclonal antibodies to rat B lymphocyte (sub-)populations. Adv. Exp. Med. Biol. 186: 81 - 89.

2. Kroese, F.G.M., A.S. Wubbena, D. Opstelten, G.J. Deenen, E.H. Schwander, L. de Leij, H. Vos, S. Poppema, J. Volberda, and P. Nieuwenhuis. 1987. B lymphocyte differentiation in the rat: production and characterization of monoclonal antibodies to B lineage-associated antigens. Eur. J. Immunol. 17: 921 - 928.

3. Opstelten, D., G.J. Deenen, J. Rozing, and S.V. Hunt. 1986. B lymphocyte-associated antigens on terminal deoxynucleotidyl transferase-positive cells and pre-B cells in bone marrow of the rat. J. Immunol. 137: 76 - 84.

4. Deenen, G.J., S.V. Hunt, and D. Opstelten. 1987. A stathmokinetic study of B lymphocytopoiesis in rat bone marrow: proliferation of cells containing cytoplasmic µ-chains, terminal deoxynucleotidyl transferase and carrying HIS24 antigen. J. Immunol. 139: 702 - 710.

5. Hermans, M.H.A., G.J. Deenen, N. de Boer, W. Bo, F.G.M. Kroese, and D. Opstelten. 1997. Expression of HIS50 ag: a rat homologue of mouse heat-stable antigen andhuman CD24 on B lymphoid cells in the rat. Immunology 90: 14 - 22.

6. Kroese, F.G.M., E.C. Butcher, P.A. Lalor, A.M. Stall, and L.A. Herzenberg. 1990. The rat B cell system: the anatomical localization of flow cytometry-defined B cell subpopulations. Eur. J. Immunol. 20: 1527 - 1534.

7. Johnson, P., A. Maiti, and D.H.W. Ng. 1997. CD45: A family of leukocyte-specific cell surface glycoproteins. In Weir's Handbook of Experimental Immunology, L.A. Herzenberg, D.M. Weir, L.A. Herzenberg, and C. Blackwell, eds. Blackwell Science, Cambridge, p. 62.1 - 62.16.

For Research Use Only