rev: March 27, 2003
HOME (index HOME page)
Return (master anti-rat CD antibody index page)
Anti-RAT
CD CLUSTERED
ANTIBODIES
RDI Division of Fitzgerald Industries Intl offers a wide line of antibodies. Since no one antibody works best for all applications (flow cytometry, neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below. All products are for in vitro research use only-not for use in or on humans or animals-not for use in diagnostics. Price/availability/specifications subject to change without notice.
Anti-RAT CD45
Antibodies
cat# RDI-RTCD45-OX $438.00/0.5mg
RDI-RTCD45-OXFT $625.00/0.5mg FITC conj
RDI-RTCD45-OXBT $500.00/0.5mg Biotin conj
RDI-RTCD45-OXPE $625.00/200ug PE conj
Presentation: 0.5mg purified antibody in 1ml (0.5mg/ml) buffer with 0.09%
sodium azide
Clone: OX-1
Immunogen: Leukocyte common antigen-enriched glycoprotein fraction from Wistar rat thymocytes
Isotype: Mouse (BALB/c) IgG1k
Purification: from tissue culture supernatant by affinity chromatography.
Use: -flow cytometry (approx 1ug/million cells)This antibody has been tested by immunofluorescent staining (1 µg/million cells)
-immunoaffinity chromatography,1,4
-immunoprecipitation
-immunofluorescent staining of epidermal sheets,5 and
immunohistochemical staining (IHC) of acetone-fixed frozen and zinc-fixed or citrate-pretreated formalin-fixed paraffin-embedded sections.
SPECIFICITY: The OX-1 antibody reacts with all molecular forms of CD45 (Leukocyte Common Antigen) on all hematopoietic cells except erythrocytes.1,2 CD45 is a member of the Protein Tyrosine Phosphatase (PTP) family: Its intracellular (COOH-terminal) region contains two PTP catalytic domains, and the extracellular region is highly variable due to alternative splicing of exons 4, 5, and 6 (designated A, B, and C, respectively), plus differing levels of glycosylation.3 The CD45 isoforms detected in the rat are cell type-, maturation-, and activation state-specific.3 The CD45 isoforms play complex roles in T-cell and B-cell antigen receptor signal transduction.3
Storage: Store 4 DEg C undiluted> Avoid frequent warming cycles
Caution: Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer.
REFERENCES
1. Sunderland, C.A., W.R. McMaster, and A.F. Williams. 1979.Purification with monoclonal antibody of a predominant leukocyte-common antigen and glycoprotein from rat thymocytes. Eur. J. Immunol. 9: 155 - 159.
2. Woollett, G.R., A.N. Barclay, M. Puklavec, and A.F. Williams. 1985. Molecular and antigenic heterogeneity of the rat leukocyte-common antigen from thymocytes and T and B lymphocytes. Eur. J. Immunol. 15: 168 - 173.
3. Johnson, P., A. Maiti, and D.H.W. Ng. 1997. CD45: A family of leukocyte-specific cell surface glycoproteins. In Weir’s Handbook of Experimental Immunology, Vol. 2. L.A. Herzenberg, D.M. Weir, L.A. Herzenberg, and C. Blackwell, eds. Blackwell Science, Cambridge, MA, pp. 62.1 - 62.16.
4. Standring, R., W.R. McMaster, C.A. Sunderland, and A.F. Williams. 1978. The predominant heavily glycosylated glycoproteins at the surface of rat lymphoid cells are differentiation antigens. Eur. J. Immunol. 8: 832 - 839.
5. Elbe, A., O. Kilgus, T. Hünig, and G. Stingl. 1993. T-cell receptor diversity in dendritic epidermal T cells in the rat. J. Invest. Dermatol. 102: 74 79.
6. Kampinga, J., and R. Aspinall. 1990. Thymocyte differentiation and thymic micro-environment development in the fetal rat thymus: an immunohistological approach. In Role of the Thymus in Tolerance Induction, Vol. 3. M.D. Kendall and M.A. Ritter, eds. Harwood Academic Publishers, New York, pp. 149 186.
Note: For In Vitro Research Use Only-not responsible for patent infringements with use or derivation of any products. w
Hazardous Ingredient: Sodium Azide. Avoid exposure to skin and eyes, ingestion, and contact with heat, acids, and metals. Wash exposed skin with soap and water. Flush eyes with water. Dilute with running water before discharge into plumbing.
Precautions: For In vitro research Use Only. Not for use in or on humans
or animals or for diagnostics. Sodium azide may form explosive compounds
in presence of heavy metals or under acidic conditions. Flush drains with
copious amounts of water to prevent buildup of explosive compounds.
34 Junction Square Drive
Concord MA 01742-3049
USA
phone (978) 371-6446 or (800) 370-2222
fax (978) 371-2266
EMAIL:antibodies@fitzgerald-fii.com