anti-RAT CD28 antibodies from RDI Division of Fitzgerald Industries Intl

rev: May 13, 2003

HOME (index HOME page)

Return (master anti-rat CD antibody index page)

Anti-RAT
CD CLUSTERED ANTIBODIES

RDI Division of Fitzgerald Industries Intl offers a wide line of antibodies. Since no one antibody works best for all applications (flow cytometry, neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below. All products are for in vitro research use only-not for use in or on humans or animals-not for use in diagnostics. Price/availability/specifications subject to change without notice.

Anti-RAT CD 28Antibodies

clone JJ319 & JJ316

DATA SHEET: Mouse anti-Rat CD28 clone JJ319

Catalog#: RDI-RTCD28-JJ319 $625.00/vial

Package Size: 500ug purified in 0.5ml PBS 1mg/ml (no carrier, no preservative) sterile filtered, endotoxin <0.01ng/ug antibody at time of bottling.

Species: mouse IgG1k

CLONE: JJ319

Immunogen: Rat cd28 transfected mouse A20JB lymphoma cell line

Source: purified

Activity: reacts with rat CD28 which is expressed on virtually all T cells bearing AlphaB T cell receptors (TCR), on most DG TCR-bearing T cells and on a subset of NK cells. In the thymus, CD28 expression is regulated during the maturation of AB TCR-bearing T cells. CD28 is a co- timulatory receptor required for activation of T cells, its ligands include CD80 (B7-1) and CD86 (B7-2). Soluble clone JJ319 antibody co-stimulated proliferative responses and IL-2 production of CD4+ and CD8+ T cells activated by anti-T cell receptor mab.

Application: -Indirect immunofluorescence cell surface staining.

-immunoprecipitation

-in vitro co-stimulation of T cell activation via TCR (clone JJ316 is capable of directly stimulating T cells in vitro and in vivo)

Storage: Store at 4 DEG C . Handle under asceptic conditions or add a preservative suitable for your system. Precautions: For In vitro research Use Only. Not or use in or on humans or animals or for diagnostics.

References: -J. Immunol 154:5121-5127

-Eur J Immunol 25:328-332

-Immunity 2:555-559

-Eur J. Immunol 27:239-247

It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.

also available: FITC labeled- cat#RDI-RTCD28-319FT $625.00/0.5mg

Biotin labeled cat#RDI-RTCD28-319BT $562.00/0.5mg

with azide formulation cat#RDI-RTCD28-319 $469.00/0.5mg

see also clone JJ316

DATA SHEET: Mouse anti-Rat CD28 clone JJ316

Catalog#: RDI-RTCD28-JJ316 $625.00/vial

Package Size: 500ug purified in 0.5ml PBS 1mg/ml (no carrier, no preservative) sterile filtered, endotoxin <0.01ng/ug antibody at time of bottling.

Species: mouse IgG1k

CLONE: JJ316

Source: purified

Activity: reacts with rat CD28 which is expressed on virtually all T cells bearing AlphaB T cell receptors (TCR), on most DG TCR-bearing T cells and on a subset of NK cells. In the thymus, CD28 expression is regulated during the maturation of AB TCR-bearing T cells. CD28 is a co- timulatory receptor required for activation of T cells, its ligands include CD80 (B7-1) and CD86 (B7-2). Soluble Clone JJ316 enhances proliferative responses and IL-2 production by T cells activated by anti-T cell receptor Mab. At hugh concentrations, cross linked clone JJ316 mab stimulates resting CD4+ and CD8+ T cells to proliferate, secrete IL-2, express activation markers, and gain CTL activity in the absence of signalling through TCR. In VIVO administration of mab JJ316 results in blastogenic transformation of peripheral CD4+ cells and dramatic lymphocytosis.

Application: -Indirect immunofluorescence cell surface staining.

-immunoprecipitation

-in vitro AND IN VIVO T cell stimulation and in vitro co- stimulation of T cell activation via TCR.

Storage: Store at 4 DEG C . Handle under asceptic conditions or add a preservative suitable for your system.

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.

References: -J. Immunol 154:5121-5127

-Eur J Immunol 27:239-247

-Eur J Immunol. 25:328-332

-Immunity 2:555-559