rev: August 8, 2002
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CD CLUSTERED ANTIBODIES
RDI Divison of Fitzgerald Industries Intl offers a wide line of antibodies. Since no one antibody works best for all applications (flow cytometry, neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below. All products are for in vitro research use only-not for use in or on humans or animals-not for use in diagnostics. Price/availability/specifications subject to change without notice.
Catalog#: RDI-RTCD31-3A $500.00/vial
Package Size: 500ug in 1ml (0.5mg/ml) 10mM PBS pH7.2 with 150mM NaCl and
Species: mouse IgG1k
Antigen: activated Lewis rat derived microglial cells
Fusion: spleen cells from immunized Balb/c mice were fused with cells of mouse SP2 myeloma cell line.
Activity: Reacts with rat (CD31)Pecam-1. Antibody gives positive reaction at high dilutions against recombinant rat PECAM-1. Reactivity with other species not determined. Non reactive with human CD31. CD31 is an approx 130kda integral membrane glycoprotein, a member of the immunoglobulin superfamily, that mediates homophilic and heterophilic cell-cell adhesion. CD31 is expressed on endothelial cells, platelets, and subsets of leukocytes. In the human and mouse, multiple alternatively spliced isoforms have been identified and this alternative splicing may be involved in the regulation of ligand specificity. CD31 mediated endothelial cell- cell interaction are involved in angiogenesis in the mouse and rat. In addition, PECAm-1 has been demonstrated to play an important role in extravasation of leukocytes invivo. Mab from clone TLD-3A12 partially blocks the proliferative response of antigen-specific CD4+ T cells to antigen presenting cells and relevant antigen. This antibody also cross reacts with Pig endothelial cells and platelets as determined by histochemistry on acetone fixed frozen sections and flow cytometry.
Application: -Indirect fluorescence (approx 1ug/million cells)
-histochemistry on acetone fixed frozen sections (approx 5-10ug/ml with ABC/Dab detection) rat spleen as positive control continuned on back side
-for paraffin sections recommend zinc fixed sections
-in vitro blocking using azide free, sterile material.
-titers must be determined for each application
Storage: Store at 4-8 DEg C
Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.
References: -J. Neurosci Res 45:747-757 (1996)
-J. Exp Med 184:229-239 (1996)
PE conjugated cat#RDI-RTCD31-3APE $438.00/100ug
Biotin conjugated cat#RDI-RTCD31-3ABT $562.00/0.5mg
no azide/sterile filtered formulation 0.5mg=cat#RDI-CD313A12-XP $625.00/0.5mg
Please inquire of other CD antibodies against rat, mouse, human and other species.
See other rat cd antibodies at: http://www.researchd.com/ratcdabs/ratcdabs.htm
Precautions: For In vitro research Use Only. Not for use in or on humans
or animals or for diagnostics It is the responsibility of the user to comply
with all local/state and Federal rules in the use of this product. We are
not responsible for any patent infringements that might result with the use
of or derivation of this product.
34 Junction Square Drive
Concord MA 01742-3049
phone (978) 371-6446 or (800) 370-2222
fax (978) 371-2266