rev:  August 8, 2002

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Anti-PIG/PORCINE
CD CLUSTERED ANTIBODIES  

RDI Divison of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (flow cytometry, neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below. All products are for in vitro research use only-not for use in or on humans or animals-not for use in diagnostics. Price/availability/specifications subject to change without notice.


Anti-PIG/PORCINE  Antibodies  (see 2 clones below)
DATA SHEET: mouse anti-Rat CD 31 clone (TLD-3A12) RAT/PIG

Catalog#: RDI-RTCD31-3A   $500.00/vial

Package Size: 500ug in 1ml (0.5mg/ml) 10mM PBS pH7.2 with 150mM NaCl and 0.09% NaN3

Species: mouse IgG1k

Antigen: activated Lewis rat derived microglial cells

Fusion: spleen cells from immunized Balb/c mice were fused with cells of mouse SP2 myeloma cell line.

CLONE: TLD-3A12

Activity: Reacts with rat (CD31)Pecam-1. Antibody gives positive reaction at high dilutions against recombinant rat PECAM-1. Reactivity with other species not determined. Non reactive with human CD31. CD31 is an approx 130kda integral membrane glycoprotein, a member of the immunoglobulin superfamily, that mediates   homophilic and heterophilic cell-cell   adhesion. CD31 is expressed on endothelial cells, platelets, and subsets of leukocytes. In the human and mouse, multiple alternatively spliced isoforms have been identified and this alternative splicing may be involved in the regulation of ligand specificity. CD31 mediated endothelial cell- cell interaction are involved in angiogenesis in the mouse and rat. In addition, PECAm-1 has been demonstrated to play an important role in extravasation of leukocytes invivo. Mab from clone TLD-3A12 partially blocks the proliferative response of antigen-specific CD4+ T cells to antigen presenting cells and relevant antigen. This antibody also cross reacts with Pig endothelial cells and platelets as determined by histochemistry on acetone fixed frozen sections and flow cytometry.

Application: -Indirect fluorescence (approx 1ug/million cells)

                       -histochemistry on acetone fixed frozen sections (approx 5-10ug/ml with ABC/Dab detection) rat spleen as positive control continuned on back side

                     -for paraffin sections recommend zinc fixed   sections

                    -western blotting

                    -immunoassay

                    -immunoprecipitation

                    -in vitro blocking using azide free, sterile material.

                   -titers must be determined for each application

Storage: Store at 4-8 DEg C

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.

References: -J. Neurosci Res 45:747-757 (1996)

                  -GLIA 7:34-40

                  -Immunology 84:453-460.

                  -J. Exp Med 184:229-239 (1996)

Also available:

PE conjugated         cat#RDI-RTCD31-3APE    $438.00/100ug

Biotin conjugated   cat#RDI-RTCD31-3ABT    $562.00/0.5mg

no azide/sterile filtered formulation   0.5mg=cat#RDI-CD313A12-XP $625.00/0.5mg

Please inquire of other CD antibodies against rat, mouse, human and other species.

See other rat cd antibodies at: http://www.researchd.com/ratcdabs/ratcdabs.htm

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.


see also anti-porcine CD1, CD2, CD3, CD4, CD6, CD8, CD11b, CD25, CD29, CD31, CD44, CD45, CD45RA, CD45RC, CD49D, CD56, CD62E, CD106, CD14, endothelial cells, HLA Class IIDQ, monocytes/macrophages, neutrophils/eosinophils, IgA, IgG, IgG1, IgG2, IgM and more...,
It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.
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RDI Divison of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com