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ANTIBODIES  

(anti-Human and others as indicated)

RDI Divison of researchd Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.

The brains of patients with Alzheimer's disease are characterised by an abundance of fibrous lesions of senile plaques, neurofibrillary tangles and neuropil threads. Tangles represent dense accumulations of ultrastructurally distinct paired helical filaments whose major component is a microtubule-associated tau protein. Tau-2, raised against the bovine tau protein, cross reacts with the human phosphorylated form of tau protein enabling the conformational dependent epitope found only in the neurofibrillary components in Alzheimer's disease cases to be detected. Therefore, Tau-2, together with B-Amyloid can aid in the positive and specific identification of Alzheimer's disease in humans.

Catalog#: RDI-TAUabm    $438.00/vial   (purified and bulk quoted on request)

Package Size: 1ml lyophilized (tissue culture supernatant containing 15mM sodium azide). Reconstitute with 1ml distilled water.

Clone: TAU-2

Isotype: mouse IgG1

Hybridoma Partner: mouse myeloma (SP2/0)

Antigen: bovine tau protein

Species Reactivity: reported with human, monkey, cow, cat and chicken

Uses: frozen sections, paraffin sections, western blot

Activity: Positive staining can be observed in human brain, Alzheimer's disease

Staining pattern: Cytoplasmic staining of neurofibriltary tangles, enuropil threads and dystrophic neurites surrounding serile plaques.

Application Typical working dilution for paraffin sections 1:25 - 1:100. 60 minute incubation at 25 DEG C. Standard ABC technique.

Storage : Store unopened lyophilised antibody at 4'C. Under these conditions, there is no significant loss in product performance up to the expiry date indicated on the vial label. The reconstituted antibody is stable for a least two months when stored at 4'C. For long term storage, it is recommended that aliquots of the antibody are frozen at -20'C (frost-free freezers are not recommended). Repeated freezing and thawing must be avoided. Prepare working dilutions on the day of use.

general ref:

Dickson D.W., Ksiezak-Reding H.,Liu W.K., et al: Immunocytochemistry of neurofibrillary tangles with antibodies to subregions of tau protein: Identification of hidden and cleaved tay epitopes and a new phosphorylation site. Acta Neuropathol. 84: 596-605 (1992).

Lang E. and Otvos L.A. Serine to proline change in the Alzheimer's disease-associated epitope tau 2 results in altered secondary structure, but phosphorylationovercomes the conformational gap. Biochemical and Biophysical ResearchCommunications. 188 (1): 162-169 (1992).

Papasozomenos S.C. Tau protein immunoreactivity in dementia of the Alzheimer type:II. Electron microscopy and pathogenetic implications. Effects of fixation on the morphology of the Alzheimer's abnormal filaments. Laboratory investigation, 60(3): 375-389 (1989).

Precautions:For In vitro research Use Only. Not for use in or on humans or animals

-Stains the human neufibrillary tangles, neutrophil threads, and neuritic plaques associated with Alheimer's disease.

Catalog#: RDI-TAU5abm       $438.00/vial 100ug in 0.5ml

                RDI-TAU5abm-1    $719.00/vial 200ug in 1.0ml

                 RDI-TAU5abm-BT $500.00/vial 100ug Biotin conjugated

Package Size: purified antibody at (200ug/ml) PBS pH 7.4 with 0.2% BSA and 15mM sodium azide.

-azide free cat#RDI-TAU5abm-1XP $750.00/200ug in 0.2ml (no BSa/No Azide) Bulk on request

Clone: TAU-5

Isotype: mouse IgG1

Antigen: purified bovine microtubule associated proteins

Activity: Positive staining can be observed in human brain, Alzheimer's disease

Staining pattern: Reacts with 45-68kDa tau proteins. No cross reaction with MAPs or tubulin. It reacts with the unphosphorylated tau and the phosphorylated tau and its epitope maps in the middle of tau. -Stains human neufibrillary tangles, neurophil threads, and neuritic plaques associated with Alzheimer's disease. It also stains astrocytes and ribosomes.

Positive control: Human T98G glioblastoma cells or brain tissue

Localization: cytoplasmic

Application -paraffin sections (enhanced staining using high temp release with boiling 10mM citrate buffer pH 6.0 for 10-20minutes followed by cooling at RT for 20 minutes), approx 2ug/ml titer, for human recommend new polyclonal cat#RDI-TAUCabRX  $438.00/0.5mg  $688.00/1ml

-western blot 1ug/ml 2 hours RT

-frozen sections and immunofluorescence 1-2ug/ml for 60 minutes rt

-immunoprecipitation 2ug/mg using Protein G

Species Reactivity: human, sheep ,cow, mouse and rat

Storage : 4 DEG C (2-8 DEG C). Freezing not recommended.

ref: LoPresti P et al, Proc Natl Acad Sci USA 1995 92(22):10369-73.

For Research use Only.

Catalog#: RDI-TAUabS   $562.00/vial

Package Size: 0.5mg in 0.5ml (1mg/ml) PBS (preservative free)

Immunogen: purified human Tau

Prepared from neat sheep serum by selective ammonium sulphate precipitation. Sterile filtered, NO PRESERVATIVES OR CARRIER PROTEINS ADDED. Endotoxin <0.002EU/ug by LAL.

Purity: >90% by SDS-Page

Species: Sheep IgG fraction.

Activity: Reacts with normal tau from human, rat and bovine brain and also with phosphorylated-tau (PHF-tau)

Application -western blot (40-60ug/ml)can detect 10ng SDS-denatured and B-mercaptoethanol reduced human tau using chemiluminescence detection

-Immunohistochemistry :20-60ug/ml for localization of PHF-tau in formalin fixed paraffin embedded brain tissue.

-All titers must be determined in assay

Storage: Store at -20 Deg C upon receipt. Avoid repeated freeze thaw cycles. A carrier protein (1% BSA) or serum should be added to diluted antibody to maintain the protein concentration at 1mg/ml or greater. This reduces antibody loss by blocking non specific binding to container surfaces.

ref: Mercken M et al, J. Neurochem 58:548 (1992)

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.  It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this products.

Catalog#: RDI-TAUabm-T2   $438.00/vial

Package Size: 100ug in 0.5ml (200ug/ml) PBS (preservative free)

Immunogen: purified bovine Tau

Isotype: mouse IgG1

Prepared from purified from serum-free culture supernatant by Protein A affinity chromatography. NO PRESERVATIVES OR CARRIER PROTEINS ADDED. Endotoxin <0.2EU/ug by LAL.

Purity: >95% by SDS-Page

Activity: Reacts with normal tau from human, rat , bovine, and monkey brain. No cross reactivity with PHF-Tau has been observed.

Epitope: binds human tau between residue 194 and 198 (numbering acording to human tau40) corresponding to the amino acid sequence RSGYS

Application -western blot (20-60ug/ml)can detect 10ng SDS-denatured and B-mercaptoethanol reduced tau using chemiluminescence detection

-ELISA: 2-10ug/ml as capturing reagent for normal tau.

-All titers must be determined in assay

Storage: Store at -20 Deg C upon receipt. Avoid repeated freeze thaw cycles. A carrier protein (1% BSA) or serum should be added to diluted antibody to maintain the protein concentration at 1mg/ml or greater. This reduces antibody loss by blocking non specific binding to container surfaces.

ref: Mercken M et al, J. Neurochem 58:548-553 (1992)

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this products.

also available Biotin conjugated:   cat#RDI-TAUabm-T2BT    $438.00/50ug

Catalog#: RDI-TAUPHabm-1    $688.00/vial

Package Size: 100ug in 0.5ml (200ug/ml) PBS (preservative free)

Immunogen: purified human PHF-Tau

Isotype: mouse IgG1

Prepared from purified from serum-free culture supernatant by Protein A affinity chromatography. NO PRESERVATIVES OR CARRIER PROTEINS ADDED. Endotoxin <0.05EU/ug by LAL.

Purity: >95% by SDS-Page

Activity: Reacts specifically recognizes PHF-tau. No cross reactivity with normal bioposy or autopsy-derived tau or fetal tau has been observed. This suggests that this antibody detects an abnormal phosphorylation site unique to PHF-tau.

Epitope: binds human tau in an epitope shown to contain phosphorylated Ser212 and phosphorylated Thr214 residues (numbering according to human Tau 40).

Application -western blot (1-5ug/ml)can detect 50ng SDS-denatured and B-mercaptoethanol reduced Phf-tau using chemiluminescence detection

-ELISA: 5-10ug/ml as capturing reagent for Phf-tau.

-histochemistry: 1-5ug/ml for localization of PHF-tau in formalin fixed paraffin embedded brain tissue.

-All titers must be determined in assay

Storage: Store at -20 Deg C upon receipt. Avoid repeated freeze thaw cycles. A carrier protein (1% BSA) or serum should be added to diluted antibody to maintain the protein concentration at 1mg/ml or greater. This reduces antibody loss by blocking non specific binding to container surfaces.

ref: -Neuroscience Lett:255, 13-16 (1998)

       -Eur J Biochem 252:542-552 (1998)

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this products.

also available Biotin conjugated: cat#RDI-TAUPHabm-1BT $688.00/50ug

Catalog#: RDI-TAUPHabm-8    $688.00/vial

Package Size: 100ug in 0.5ml (200ug/ml) PBS (preservative free)

Immunogen: partially purified human PHF-Tau

Isotype: mouse IgG1

Clone: AT8

Prepared from purified from serum-free culture supernatant by Protein A affinity chromatography. NO PRESERVATIVES OR CARRIER PROTEINS ADDED. Endotoxin <0.1EU/ug by LAL.

Purity: >95% by SDS-Page

Activity: Specifically recognizes a phosphate sensitive epitope on PHF-Tau. No croess reactivity with normal tau has been observed.PHF-tau.

Epitope: The epitope of this antibody contains the phosphorylated Ser202 residue (numbering according to human Tau 40).

Application -western blot (20-60ug/ml)can detect 50ng SDS-denatured and B-mercaptoethanol reduced Phf-tau using chemiluminescence detection

-ELISA: 5-10ug/ml as capturing reagent for Phf-tau.

-histochemistry: 5-10ug/ml for localization of PHF-tau in formalin fixed paraffin embedded brain tissue.

-All titers must be determined in assay

Storage: Store at -20 Deg C upon receipt. Avoid repeated freeze thaw cycles. A carrier protein (1% BSA) or serum should be added to diluted antibody to maintain the protein concentration at 1mg/ml or greater. This reduces antibody loss by blocking non specific binding to container surfaces.

ref: -Acta Neuropathol 87:554-567 (1994)

-J. Neurochem 61:1828-1834 (1993)

-EMBO Jour 11:1593-1597 (1992)

-Acta Neuropathol 84:265-272 (1992)

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this products.

also available Biotin conjugated: cat#RDI-TAUPHabm-8BT $688.00/50ug

RDI Division of researchdIndustires Intl

(Research Diagnostics Inc)

San Jose, 95123 CA Snell ave 658

USA

or (800) 370-2222

email: sales@researchd.com

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