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ANTIBODIES
(anti-Human and others as indicated)
RDI Division of Fitzgerald Industries Intl offers a wide line of antibodies. Since no one antibody works best for all applications (neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.
see below monoclonal and polyclonal to Tau and phosphorylated Tau
DATA SHEET: Mouse anti-TAU monoclonal
The brains of patients with Alzheimer's disease are characterised by an abundance of fibrous lesions of senile plaques, neurofibrillary tangles and neuropil threads. Tangles represent dense accumulations of ultrastructurally distinct paired helical filaments whose major component is a microtubule-associated tau protein. Tau-2, raised against the bovine tau protein, cross reacts with the human phosphorylated form of tau protein enabling the conformational dependent epitope found only in the neurofibrillary components in Alzheimer's disease cases to be detected. Therefore, Tau-2, together with B-Amyloid can aid in the positive and specific identification of Alzheimer's disease in humans.
Catalog#: RDI-TAUabm $438.00/vial
(purified and bulk quoted on request)
Package Size: 1ml lyophilized (tissue culture supernatant containing 15mM
sodium azide). Reconstitute with 1ml distilled water.
Clone: TAU-2
Isotype: mouse IgG1
Hybridoma Partner: mouse myeloma (SP2/0)
Antigen: bovine tau protein
Species Reactivity: reported with human, monkey, cow, cat and chicken
Uses: frozen sections, paraffin sections, western blot
Activity: Positive staining can be observed in human brain, Alzheimer's disease
Staining pattern: Cytoplasmic
staining of neurofibriltary tangles, enuropil threads and dystrophic neurites
surrounding serile plaques.
Application Typical working dilution for paraffin sections 1:25 - 1:100. 60
minute incubation at 25 DEG C. Standard ABC technique.
Storage : Store unopened lyophilised
antibody at 4'C. Under these conditions, there is no significant loss in
product performance up to the expiry date indicated on the vial label. The
reconstituted antibody is stable for a least two
months when stored at 4'C. For long term storage, it is recommended that
aliquots of the antibody are frozen at -20'C (frost-free freezers are not
recommended). Repeated freezing and thawing must be avoided. Prepare working
dilutions on the day of use.
general ref:
Dickson D.W., Ksiezak-Reding H.,Liu
W.K., et al: Immunocytochemistry of neurofibrillary tangles with antibodies to subregions of tau protein: Identification of hidden and
cleaved tay epitopes and a
new phosphorylation site. Acta Neuropathol.
84: 596-605 (1992).
Lang E. and Otvos L.A. Serine to proline
change in the Alzheimer's disease-associated epitope
tau 2 results in altered secondary structure, but phosphorylationovercomes
the conformational gap. Biochemical and Biophysical ResearchCommunications. 188 (1): 162-169 (1992).
Papasozomenos S.C. Tau protein immunoreactivity in dementia of the Alzheimer type:II. Electron microscopy and pathogenetic implications. Effects of fixation on the morphology of the Alzheimer's abnormal filaments. Laboratory investigation, 60(3): 375-389 (1989).
Precautions:For In vitro
research Use Only. Not for use in or on humans or animals
DATA SHEET: Mouse anti-TAU monoclonal (Clone Tau-5)
-Stains the human neufibrillary tangles, neutrophil threads, and neuritic plaques associated with Alheimer's disease.
Catalog#: RDI-TAU5abm, new cat#: 10R-T101ax $484.00/100ug
RDI-TAU5abm-1, new cat#: 10R-T101a $756.00/200ug
RDI-TAU5abm-BT $500.00/vial 100ug Biotin conjugated
Package Size: purified antibody at (200ug/ml) PBS pH 7.4 with 0.2% BSA and 15mM sodium azide.
-azide free cat#RDI-TAU5abm-1XP $750.00/200ug in 0.2ml (no BSa/No Azide) Bulk on request
Clone: TAU-5
Isotype: mouse IgG1
Antigen: purified bovine microtubule associated proteins
Activity: Positive staining can be observed in human brain, Alzheimer's
disease
Staining pattern: Reacts with 45-68kDa tau proteins.
No cross reaction with MAPs or tubulin.
It reacts with the unphosphorylated tau and the phosphorylated tau and its epitope
maps in the middle of tau. -Stains human neufibrillary
tangles, neurophil threads, and neuritic
plaques associated with Alzheimer's disease. It also stains astrocytes
and ribosomes.
Positive control: Human T98G glioblastoma cells or
brain tissue
Localization: cytoplasmic
Application -paraffin sections (enhanced staining using high temp release with
boiling 10mM citrate buffer pH 6.0 for 10-20minutes followed by cooling at RT
for 20 minutes), approx 2ug/ml titer, for human recommend new polyclonal cat#RDI-TAUCabRX $438.00/0.5mg $688.00/1ml
-western blot 1ug/ml 2 hours RT
-frozen sections and immunofluorescence 1-2ug/ml for 60 minutes rt
-immunoprecipitation 2ug/mg using Protein G
Species Reactivity: human, sheep ,cow, mouse and rat
Storage : 4 DEG C (2-8 DEG C). Freezing not recommended.
ref: LoPresti P et al,
Proc Natl Acad Sci
For Research use Only.
DATA SHEET: Sheep anti-Tau, polyclonal
Catalog#: RDI-TAUabS $562.00/vial
Package Size: 0.5mg in 0.5ml (1mg/ml) PBS (preservative free)
Immunogen: purified human Tau
Prepared from neat sheep serum by selective ammonium sulphate
precipitation. Sterile filtered, NO PRESERVATIVES OR CARRIER PROTEINS ADDED. Endotoxin <0.002EU/ug by LAL.
Purity: >90% by SDS-Page
Species: Sheep IgG fraction.
Activity: Reacts with normal tau from human, rat and bovine brain and also with
phosphorylated-tau (PHF-tau)
Application -western blot (40-60ug/ml)can detect 10ng SDS-denatured and B-mercaptoethanol reduced human tau using chemiluminescence detection
-Immunohistochemistry :20-60ug/ml for localization of PHF-tau in formalin fixed paraffin embedded brain tissue.
-All titers must be determined in assay
Storage: Store at -20 Deg C upon receipt. Avoid repeated freeze thaw cycles. A
carrier protein (1% BSA) or serum should be added to diluted antibody to
maintain the protein concentration at 1mg/ml or greater. This reduces antibody
loss by blocking non specific binding to container surfaces.
ref: Mercken M et al, J. Neurochem
58:548 (1992)
Precautions: For In vitro research Use Only. Not for use in or on humans or
animals or for diagnostics. It is the responsibility of the user to
comply with all local/state and Federal rules in the use of this product. We
are not responsible for any patent infringements that might result with the use
of or derivation of this products.
DATA SHEET: Mouse anti-Tau, monoclonal
Catalog#: RDI-TAUabm-T2, new cat#: 10R-T102a
$484.00/vial
Package Size: 100ug in 0.5ml (200ug/ml) PBS (preservative free)
Immunogen: purified bovine Tau
Isotype: mouse IgG1
Prepared from purified from serum-free culture supernatant by Protein A affinity
chromatography. NO PRESERVATIVES OR CARRIER PROTEINS ADDED. Endotoxin
<0.2EU/ug by LAL.
Purity: >95% by SDS-Page
Activity: Reacts with normal tau from human, rat , bovine, and monkey brain. No
cross reactivity with PHF-Tau has been observed.
Epitope: binds human tau between residue 194 and 198
(numbering acording to human tau40) corresponding to
the amino acid sequence RSGYS
Application -western blot (20-60ug/ml)can detect 10ng SDS-denatured and B-mercaptoethanol reduced tau using chemiluminescence
detection
-ELISA: 2-10ug/ml as capturing reagent for normal tau.
-All titers must be determined in assay
Storage: Store at -20 Deg C upon receipt. Avoid repeated freeze thaw cycles. A
carrier protein (1% BSA) or serum should be added to diluted antibody to
maintain the protein concentration at 1mg/ml or greater. This reduces antibody
loss by blocking non specific binding to container surfaces.
ref: Mercken M et al, J. Neurochem
58:548-553 (1992)
Precautions: For In vitro research Use Only. Not for use in or on humans or
animals or for diagnostics. It is the responsibility of the user to comply with
all local/state and Federal rules in the use of this product. We are not
responsible for any patent infringements that might result with the use of or derivation
of this products.
also available Biotin conjugated: cat#RDI-TAUabm-T2BT $438.00/50ug
DATA SHEET: Mouse anti-HUMAN Phosphorylated Tau (SER212/214) , monoclonal
Catalog#: RDI-TAUPHabm-1 $688.00/vial
Package Size: 100ug in 0.5ml (200ug/ml) PBS (preservative free)
Immunogen: purified human PHF-Tau
Isotype: mouse IgG1
Prepared from purified from serum-free culture supernatant by Protein A
affinity chromatography. NO PRESERVATIVES OR CARRIER PROTEINS ADDED. Endotoxin <0.05EU/ug by LAL.
Purity: >95% by SDS-Page
Activity: Reacts specifically recognizes PHF-tau. No cross reactivity with
normal bioposy or autopsy-derived tau or fetal tau
has been observed. This suggests that this antibody detects an abnormal phosphorylation site unique to PHF-tau.
Epitope: binds human tau in an epitope
shown to contain phosphorylated Ser212 and phosphorylated Thr214 residues (numbering according to
human Tau 40).
Application -western blot (1-5ug/ml)can detect 50ng SDS-denatured and B-mercaptoethanol reduced Phf-tau
using chemiluminescence detection
-ELISA: 5-10ug/ml as capturing reagent for Phf-tau.
-histochemistry: 1-5ug/ml for localization of PHF-tau in formalin fixed paraffin embedded brain tissue.
-All titers must be determined in assay
Storage: Store at -20 Deg C upon receipt. Avoid repeated freeze thaw cycles. A
carrier protein (1% BSA) or serum should be added to diluted antibody to
maintain the protein concentration at 1mg/ml or greater. This reduces antibody
loss by blocking non specific binding to container surfaces.
ref: -Neuroscience Lett:255, 13-16 (1998)
-Eur J Biochem
252:542-552 (1998)
Precautions: For In vitro research Use Only. Not for use in or on humans or
animals or for diagnostics. It is the responsibility of the user to comply with
all local/state and Federal rules in the use of this product. We are not
responsible for any patent infringements that might result with the use of or
derivation of this products.
also available Biotin conjugated: cat#RDI-TAUPHabm-1BT $688.00/50ug
DATA SHEET: Mouse anti-HUMAN Phosphorylated Tau (Ser202), monoclonal
Catalog#: RDI-TAUPHabm-8, new cat#: 10R-T102b $724.50/100ug
Package Size: 100ug in 0.5ml (200ug/ml) PBS (preservative free)
Immunogen: partially purified human PHF-Tau
Isotype: mouse IgG1
Clone: AT8
Prepared from purified from serum-free culture supernatant by Protein A
affinity chromatography. NO PRESERVATIVES OR CARRIER PROTEINS ADDED. Endotoxin <0.1EU/ug by LAL.
Purity: >95% by SDS-Page
Activity: Specifically recognizes a phosphate sensitive epitope
on PHF-Tau. No croess reactivity with normal tau has
been observed.PHF-tau.
Epitope: The epitope of
this antibody contains the phosphorylated Ser202
residue (numbering according to human Tau 40).
Application -western blot (20-60ug/ml)can detect 50ng SDS-denatured and B-mercaptoethanol reduced Phf-tau
using chemiluminescence detection
-ELISA: 5-10ug/ml as capturing reagent for Phf-tau.
-histochemistry: 5-10ug/ml for localization of PHF-tau in formalin fixed paraffin embedded brain tissue.
-All titers must be determined in assay
Storage: Store at -20 Deg C upon receipt. Avoid repeated freeze thaw cycles. A
carrier protein (1% BSA) or serum should be added to diluted antibody to
maintain the protein concentration at 1mg/ml or greater. This reduces antibody
loss by blocking non specific binding to container surfaces.
ref: -Acta Neuropathol
87:554-567 (1994)
-J. Neurochem 61:1828-1834 (1993)
-EMBO Jour 11:1593-1597 (1992)
-Acta Neuropathol
84:265-272 (1992)
Precautions: For In vitro research Use Only. Not for use in or on humans or
animals or for diagnostics. It is the responsibility of the user to comply with
all local/state and Federal rules in the use of this product. We are not
responsible for any patent infringements that might result with the use of or
derivation of this products.
also available Biotin conjugated: cat#RDI-TAUPHabm-8BT $688.00/50ug
RDI Division of Fitzgerald Industires Intl
(Research Diagnostics Inc)
phone (978) 371-6446 or (800) 370-2222
fax (978) 371-2266
email: sales@researchd.com
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