rev:    June 18, 2001

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ANTIBODIES  

(anti-Human and others as indicated)

RDI Division of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.

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Product:               Rabbit anti-Neurofibrillary Tangle Antiserum

Cat No:                RDI-TANGLEabR  $375.00/0.25ml

Host:                   Rabbit

Clone:                  Polyclonal

Immunogen:          Disaggregated paired helical filaments

 Form:                  Whole serum with 0.1% sodium azide

                          -no azide cat#RDI-TANGLEabr-XP  $1250.00/1ml

Concentration:      N/A

Titer:                   Reported working dilution using neat serum:*

v    ELISA using immunizing peptide: 1:4,000

v    IHC using formalin-fixed, paraffin-embedded sections:

v    1:200

v    IHC using paraformaldehyde-fixed, frozen sections: 1:2,000

v    Western blots: approx 1:500

Specificity:           Human paired helical filaments from Alzheimer neurofibrillary tangles.

Storage:               Short term: Refrigerate at 4º C

Long term: Freeze at -20º C. (recommend aliquoting and store –20 DEG C. Avoid frequent freeze thaw cycles.

  FOR RESEARCH AND MANUFACTURING USE ONLY

 * Various assay conditions require that the optimum working concentrations be determined by serial dilutions of this product.

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Product: Human Neurofibrillary Tangle Antiserum

 The neurofibrillary tangles (NFT) are neuronal inclusions that are the hallmark lesions of Alzheimer’s disease (AD).  These tangles are made up of paired helical filaments (PHF).  The PHF are highly insoluble structures and are known to be composed of a highly phosphorylated form of the microtubule-associated protein tau and may contain associated lipid. Insoluble NFT which were extracted in boiling sodium dodecyl sulfate, purified by sucrose gradient centrifugation and disaggregated by sonication were used as an immunogen for the preparation of this antiserum.

 A rabbit antiserum to disaggregated PHF from AD NFT is currently available. This antiserum has been shown to be immunoreactive with disaggregated PHF by ELISA. This antiserum specifically immunostains NFT and degenerating plaque neurites in cases of AD, Down's syndrome and normal aged individuals.  NFT are heterogenous in composition and their composition undergoes changes as the tangle matures. Because monoclonal antibodies are unique to a single epitope, they tend to immunolabel subpopulations of tangles, which contain this epitope. For this reason, a monoclonal antibody that immunolabels intraneuronal tangles, may not immunolabel extracellular tangles. Polyclonal antisera to disaggregated PHF will immunolabel all NFT, both intracellular and extracellular, as well as all PHF-containing neurites. This antiserum works on formalin-fixed, paraffin-embedded sections, formalin-fixed vibratome sections and paraformaldehyde-fixed, frozen sections. This antibody should be a valuable tool for scientists working to understand the role of in AD.

  Rabbit Neurofibrillary Tangle Protein Antiserum

Cat No. RDI-TANGLEabr 

Alzheimer disease brain showing immunohistochemistry staining of neurofibrillary tangles and dystrophic neurites of senile plaques at 1:200 dilution in formalin-fixed, paraffin- embedded section of hippocampus.

Western blot analysis of Alzheimer disease brain and a purified preparation of disaggregated paired helical filaments (PHF).  The majority of insoluble PHF are excluded from the origin of the stacking gel (O1) with the rest being excluded from the origin of the separating gel (O2).

For Research Use Only

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RDI Division of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

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