rev:April 14, 2001
HOME
(index page)
Return
( alphabetical antibody index page)
Return
(neurohistology ab index page)
ANTIBODIES
(anti-Human and others as indicated)
RDI Divison of researchd Industries Intl offers a wide line of antibodies. Since no
one antibody works best for all applications (neutralization, blotting,
histochemistry, ELISA, etc), we offer many different types of antibodies to
help solve this problem. Please inquire for other applications or types of
antibodies not listed below.
Product:
Synphilin-1 C-terminal Antiserum
Cat
No:
RDI-SYNPHILabG
$315.00/250�l
Host:
Goat
Clone: Polyclonal
Immunogen:
Synthetic Peptide: 829 SLELNGEKDKDKGRTLQRT847
Form:
Whole serum
Concentration:
N/A
Supplied
as:
0.25ml
Titer:
Reported working dilution using neat serum:*
v
ELISA using immunizing
peptide:
1:10,655
v
IHC using
formalin-fixed, paraffin-embedded
sections:
1:400
v
IHC using
paraformaldehyde-fixed, frozen sections: N/D
v
Western blots:
1:2,000
Specificity:
Amino acid sequence 829 - 847 of human synphilin-1
protein.
Preservative:
0.1% Sodium Azide
(no azide available
$938.00/1ml, cat#RDI-SYNPHILabgX
Storage:
Short term: Refrigerate at
4�
C
Long term: Freeze at
-20�
C.
FOR
RESEARCH AND MANUFACTURING USE ONLY
* Various assay conditions require
that the optimum working concentrations be determined by serial dilutions
of this product.

Product: Human Synphilin-1 C-terminal Antiserum
Synphilin-1
is a cytoplasmic protein that is enriched in the neurophil of normal human
brain. Although the exact function
of synphilin-1 is unknown, it may possibly have a role as a cytoskeletal
protein based on the presence of ankyrin-like repeats and an ATP, GTP-binding
domain. It has been shown to be novel is its ability to associate
in vivo with
�-synuclein to form
cytoplasmic eosinophilic inclusions.
Additionally, it has been observed to be present in Lewy bodies of Parkinson disease,
which are composed of
�-synuclein. Because
synphilin-1 is not found in protein aggregates of Alzheimer�s disease, it
appears to be specific for
�-synuclein-containing
lesions. Synphilin-1 may
facilitate the aggregation of
�
-synuclein into Lewy bodies
A goat
antiserum to a synthetic peptide that corresponds to amino acids 829-847 of the
C-terminus of the human synphilin-1 is currently available. This antiserum has
been shown to be immunoreactive with the unconjugated immunizing peptide by
ELISA. It also labels Lewy bodies in Parkinson�s disease using formalin-fixed,
paraffin-embedded sections and it labels a 90 kD in western blots.
This antibody should be a valuable tool for scientists working to understand
the role of synphilin-1 in Parkinson�s disease.
This
antiserum was produced using proprietary methodology whereby the peptide is
attached to a carrier that elicits minimal immunoreactivity so that the
antiserum has a higher degree of specificity for the peptide.
Since there is no overwhelming production of interfering antibodies to the carrier,
this antiserum can routinely be used without further purification. A
control immune serum from a mock immunized animal is available to be used in
conjunction with this antibody as well as the immunizing peptide (Cat no.
RDI-SYNPHIL-CPX
$375.00/1mg) which can be used to neutralize immunoreactivity.
Synphilin-1 C-terminal Peptide Antiserum
Parkinson
disease brain showing immunohistochemical staining of Lewy body
in formalin-fixed, parafin- embedded section of substantia
nigra.
Western blot analysis of Alzheimer
disease
(A)
and normal aged control brain (N) using
synphilin-1 antiserum at 1:2,000
dilution.
Synphilin-1 is observed to have
a
molecular weight of 90 kD.
For Research Use
Only
RDI Divison of researchd Industries Intl
San Jose, 95123 CA Snell ave 658
USA
or 408-780-0908
EMAIL:margaret@cellular-research.com
RETURN
(neurohistology ab page)
Return ( alphabetical
antibody index page)