rev:April 14, 2001

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ANTIBODIES  

(anti-Human and others as indicated)

RDI Divison of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.

Product:               Synphilin-1 C-terminal Antiserum

Cat No:                RDI-SYNPHILabG   $315.00/250µl

 Host:                   Goat

 Clone:                 Polyclonal

 Immunogen:          Synthetic Peptide: 829 SLELNGEKDKDKGRTLQRT847

 Form:                  Whole serum

 Concentration:      N/A

 Supplied as:          0.25ml

Titer:                   Reported working dilution using neat serum:*

v    ELISA using immunizing peptide: 1:10,655

v    IHC using formalin-fixed, paraffin-embedded sections:  1:400

v    IHC using paraformaldehyde-fixed, frozen sections: N/D

v    Western blots: 1:2,000

Specificity:           Amino acid sequence 829 - 847 of human synphilin-1 protein.

Preservative:                 0.1% Sodium Azide

                          (no azide available  $938.00/1ml, cat#RDI-SYNPHILabgX

Storage:               Short term: Refrigerate at 4º C

Long term: Freeze at -20º C.

 FOR RESEARCH AND MANUFACTURING USE ONLY

 * Various assay conditions require that the optimum working concentrations be determined by serial dilutions of this product.

       
Product: Human Synphilin-1 C-terminal Antiserum

 Synphilin-1 is a cytoplasmic protein that is enriched in the neurophil of normal human brain.  Although the exact function of synphilin-1 is unknown, it may possibly have a role as a cytoskeletal protein based on the presence of ankyrin-like repeats and an ATP, GTP-binding domain. It has been shown to be novel is its ability to associate in vivo with ±-synuclein to form cytoplasmic eosinophilic inclusions.  Additionally, it has been observed to be present in Lewy bodies of Parkinson disease, which are composed of ±-synuclein.  Because synphilin-1 is not found in protein aggregates of Alzheimer’s disease, it appears to be specific for ±-synuclein-containing lesions.  Synphilin-1 may facilitate the aggregation of ± -synuclein into Lewy bodies

 A goat antiserum to a synthetic peptide that corresponds to amino acids 829-847 of the C-terminus of the human synphilin-1 is currently available. This antiserum has been shown to be immunoreactive with the unconjugated immunizing peptide by ELISA. It also labels Lewy bodies in Parkinson’s disease using formalin-fixed, paraffin-embedded sections and it labels a 90 kD in western blots.  This antibody should be a valuable tool for scientists working to understand the role of synphilin-1 in Parkinson’s disease.

 This antiserum was produced using proprietary methodology whereby the peptide is attached to a carrier that elicits minimal immunoreactivity so that the antiserum has a higher degree of specificity for the peptide.  Since there is no overwhelming production of interfering antibodies to the carrier, this antiserum can routinely be used without further purification.  A control immune serum from a mock immunized animal is available to be used in conjunction with this antibody as well as the immunizing peptide (Cat no. RDI-SYNPHIL-CPX  $375.00/1mg) which can be used to neutralize immunoreactivity.

  Synphilin-1 C-terminal Peptide Antiserum

 Parkinson disease brain showing immunohistochemical staining of Lewy body in formalin-fixed, parafin- embedded section of substantia nigra.

  

Western blot analysis of Alzheimer disease

 (A) and normal aged control brain (N) using

synphilin-1 antiserum at 1:2,000 dilution.

Synphilin-1 is observed to have a

molecular weight of 90 kD.

For Research Use Only

RDI Divison of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

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