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ANTIBODIES  

(anti-Human and others as indicated)

RDI Division of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.

Mab to Synaptophysin

cat#RDI-PRO61012, new cat#: 10R-S124a        $412.50/50ug

Clone Determination       SY 38

Category                         Mouse monoclonal

Immunoglobulin Class    IgG1

Purification                   SpAss-Protein A (recombinant)

Antigen                        Synaptophysin (presynaptic vesicles)

Specificity                  SY 38 represents an excellent marker for several neuroendocrine, neuronal and adrenal tumors


Application                Suitable for frozen and paraffin- embedded tissue (P)

                                  Cytological material

                                  FACS analysis

                                  Immunoblotting (Western)

Stability at 4°C one year after reconstitution

Quantity 50 µg (lyoph.) contain 0.1% NaN3 and 0.05% BSA

Polypeptide Reacting MR 38 000 transmembrane glyco- protein of presynaptic vesicles

Antigen Recognized in          Human, bovine, rat, mouse

Species (tested so far)

Reactivities on Cultured            Rat PC-12 cell line

Cell Lines (tested so far)

Tumors Specifically Detected   Neuronal and adrenal tumors such as pheochromocytomas, para-gangli-omas,                                                                     neuroblastomas, ganglio-neuroblastomas. Neuroendocrine tumors of epithelial origin:                                                      Pancreatic islet cell CA, bronchial and gastro-intestinal carcinoids, medullary CA of                                                        thyroid

Application                            Suitable for frozen and paraffin- embedded tissue (P)

                                              Cytological material

                                              FACS analysis

                                               Immunoblotting (Western)

Pretreatment In no case protease pretreatment

Working Dilution When reconstituted with 1ml distilled water, dilute further 1:50

Incubation Time 1h at RT; extended with P (over night at 4°C)


References

Gould, V.E., Wiedenmann, B., Lee, I., Schwechheimer, K., Dockhorn-Dworniczak, B., Radosevich, J.A., Moll, R. and Franke, W.W.: Synaptophysin expression in neuroendocrine neoplasms as determined by immunocytochemistry. Am. J. Pathol. 126, 243-257 (1987)

Gould, V.E., Lee, I., Wiedenmann, B., Moll, R., Chejfec, G. and Franke, W.W.: Synaptophysin: A novel marker for neurons, certain neuroendocrine cells and their neoplasms. Human Pathol. 17, 979-983 (1986)

Gould, V.E.: Synaptophysin - A new and promising Pan-neuroendocrine marker. Arch Pathol Lab Med 111, 791-794, (1987)

Kelly, R.B., Buckley, K.M., Burgess, T.L., Carlson, S.S., Caroni, P., Hooper, J.E., Katzen, A., Moore, H.-P., Pfeffer, S.R. and Schroer, T.A.: Membrane traffic in neurons and peptide-secreting cells. Cold Spring Harbor Symp. Quant. Biol. 48, 697-705 (1983)

Wiedenmann, B., Franke, W.W., Kuhn, C., Moll, R. and Gould, V.E.: Synaptophysin: A marker protein for neuroendocrine cells and neoplasms. Proc. Natl. Acad. Sci. USA 83, 3500-3504 (1986)

Wiedenmann, B. and Franke, W.W.: Identification and localization of synaptophysin, an integral membrane glycoprotein of Mr 38,000 characteristic of presynaptic vesicles. Cell 45, 1017-1028 (1985)

For research use only. Not supplied for use in human diagnostic or therapeutic procedures

RDI Division of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

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