rev:  February 7, 2005

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ANTIBODIES  

                                                                  (anti-Human and others as indicated)

RDI Division of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.


Prion Antibodies ( polyclonal
see also new monoclonal anti-Prion clone 1E4
PRODUCT: HUMAN PRION PrP27-30 PEPTIDE ANTISERUM

Cat. No. cat#RDI-PRIONabG   $315.00/250µl

A goat antiserum to a synthetic peptide that corresponds to amino acids 79-97 of the N-terminus of the human prion protein PrP27-30 is currently available. The prion protein is a large membrane protein that occurs normally in neurons of the human brain and is thought to be involved in synaptic transmission. In prion diseases, such as Creutzfeld-Jakob disease (CJD), Gerstmann-Straussler-Scheinker syndrome (GSS), Fatal Familial Insomnia (FFI), Alpers Syndrome and Kuru, the normal cellular form of this protein (PrPc) is transformed into an altered protein when it comes into contact with an infectious prion protein (PrPsc) from another host. This altered PrPsc accumulates in cytoplasmic vesicles of diseased individuals forming lesions, vacuoles and amyloid deposits.

PrPsc is a proteolytic-resistant form of PrPc, although no major chemical differences between the two have been found. The major difference between the two is that the infectious form has assumed a different conformational 3-D structure. The PrPc protein is highly conserved across many species, including humans, sheep, mice, hamsters, Drosophila and bovine.

The prion protein has received considerable attention in the last few years because it is the same protein that is responsible for bovine spongiform encephalopathy or AMad Cow disease@ and also scrapie in sheep. Prion disease can either occur spontaneously by means of coming in contact with the infectious agent, such as the outbreak in England with the infection of several people who consumed contaminated beef, or it can occur as a rare hereditary form. Currently, there is no cure for prion diseases and death usually occurs within one year of the onset of symptoms, which usually include dementia. This antiserum has been shown to be immunoreactive to the immunizing peptide by ELISA. The antiserum will immunolabel amyloid plaques in formalin-fixed paraffin sections from CJD brain. This antibody should be useful to scientists and neuropathologists who study the pathogenesis of prion diseases.

Reference: Shinagawa M, Munekata E, Doi, S, et al. Immunoreactivity of a synthetic pentadecapeptide corresponding to the N-terminal region of the scrapie prion protein. J gen Virol 67: 1745-1750, 1986.


DATA SHEET: POLYCLONAL ANTISERUM

DESCRIPTION: Prion PrP27-30 Peptide Antiserum

PRODUCT NO: cat#RDI-PRIONabG   $312.00/0.25ml

LOT NO:

ANTIBODY HOST: Goat

IMMUNOGEN: Synthetic peptide corresponding to amino acids 79-97 of the N-terminus of the human Prion protease-resistant protein27-30 (PrP27-30)

Sequence: G Q G G G T H S Q W N K P S K P K T N   Cat#RDI-PRION-CPX $469.00/1mg



FORM: Whole antiserum

CONCENTRATION: N/A

TITER: Recommended dilutions based on neat serum:

             - ELISA using immunizing peptide: 1:35,000

             - IHC formalin-fixed, paraffin sections: 1:200

              - IHC paraformaldehyde-fixed, frozen sections: N/D

               - Western blots: N/D

SPECIFICITY: Specific for residues 79-97 on the N-terminus of Prion PrP27-30.

STORAGE: 4° C short term; -20° C long term. Recommend aliquoting, avoid freqeust freeze thaw cycles.

For In Vitro Research Use Only


Goat Prion PrP27-30 Antiserum

Cat No. RDI-PRIONabG

Creutzfeldt-Jakob Disease brain showing immunohistochemical staining of prion plaque at 1:200 dilution in   formalin-fixed, paraffin-embedded section of cerebral cortex.


 RDI Division of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

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