rev: 5/15/2008
HOME (index page)
Return ( alphabetical antibody index page)
Return (neurohistology ab index page)
ANTIBODIES
(anti-Human and others as indicated)
RDI Division of Fitzgerald Industries Intl offers a wide line of antibodies. Since no one antibody works best for all applications (neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.
Prion Antibodies ( polyclonal
see also new monoclonal anti-Prion clone 1E4
PRODUCT: HUMAN PRION PrP27-30 PEPTIDE ANTISERUM
Cat. No. cat#RDI-PRIONabG, new cat#: 20R-PG009 $346.50/250µl
A goat antiserum to a synthetic peptide that corresponds to amino acids 79-97
of the N-terminus of the human prion protein PrP27-30 is currently available.
The prion protein is a large membrane protein that occurs normally in neurons
of the human brain and is thought to be involved in synaptic transmission. In
prion diseases, such as Creutzfeld-Jakob disease (CJD),
Gerstmann-Straussler-Scheinker syndrome (GSS), Fatal Familial Insomnia (FFI),
Alpers Syndrome and Kuru, the normal cellular form of this protein (PrPc) is
transformed into an altered protein when it comes into contact with an
infectious prion protein (PrPsc) from another host. This altered PrPsc
accumulates in cytoplasmic vesicles of diseased individuals forming lesions,
vacuoles and amyloid deposits.
PrPsc is a proteolytic-resistant form of PrPc, although no major chemical
differences between the two have been found. The major difference between the
two is that the infectious form has assumed a different conformational 3-D
structure. The PrPc protein is highly conserved across many species, including
humans, sheep, mice, hamsters, Drosophila and bovine.
The prion protein has received considerable attention in the last few years
because it is the same protein that is responsible for bovine spongiform
encephalopathy or AMad Cow disease@ and also scrapie in sheep. Prion disease
can either occur spontaneously by means of coming in contact with the
infectious agent, such as the outbreak in
Reference: Shinagawa M, Munekata E, Doi, S, et al. Immunoreactivity of a
synthetic pentadecapeptide corresponding to the N-terminal region of the
scrapie prion protein. J gen Virol 67: 1745-1750, 1986.
DATA SHEET: POLYCLONAL ANTISERUM
DESCRIPTION: Prion PrP27-30 Peptide Antiserum
PRODUCT NO: cat#RDI-PRIONabG $312.00/0.25ml
ANTIBODY HOST: Goat
IMMUNOGEN: Synthetic peptide corresponding to amino acids 79-97 of the
N-terminus of the human Prion protease-resistant protein27-30 (PrP27-30)
Sequence: G Q G G G T H S Q W N K P S K P K T N Cat#RDI-PRION-CPX $469.00/1mg
FORM: Whole antiserum
CONCENTRATION: N/A
TITER: Recommended dilutions based on neat serum:
- ELISA using immunizing peptide: 1:35,000
- IHC formalin-fixed, paraffin sections: 1:200
- IHC paraformaldehyde-fixed, frozen sections: N/D
- Western blots: N/D
SPECIFICITY: Specific for residues 79-97 on the N-terminus of Prion PrP27-30.
STORAGE: 4° C short term; -20° C long term. Recommend aliquoting, avoid
freqeust freeze thaw cycles.
For In Vitro Research Use Only
Goat Prion PrP27-30 Antiserum
Cat No. RDI-PRIONabG
Creutzfeldt-Jakob Disease brain
showing immunohistochemical staining of prion plaque at 1:200 dilution in
formalin-fixed, paraffin-embedded section of cerebral cortex.
RDI Division of Fitzgerald Industries Intl
phone (978) 371-6446 or (800) 370-2222
fax (978) 371-2266
EMAIL:antibodies@fitzgerald-fii.com
RETURN (neurohistology ab page)
Return (alphabetical antibody page)