rev: July 15, 1999

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ANTIBODIES  

                                                                  (anti-Human and others as indicated)

RDI Divison of researchd Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.


PRODUCT: PRESENILIN-2 (STM2) PEPTIDE ANTISERUM

Cat. No. RDI-PRESEN2abG     $312.00/0.25ml


A goat antiserum to a synthetic peptide that corresponds to amino acids 31-47 of the N-terminus of the Alzheimer disease presenilin-2 protein is currently available. The presenilin-2 gene was discovered on chromosome 1 and is responsible for an early onset form of autosomal dominant Alzheimer's disease. The amino acid sequence is approximately 67% identical with that of the presenilin-1 protein. It also is a membrane protein that has regions where mutations have been identified in family members with Alzheimer's disease. This peptide antibody corresponds to the extracellular portion of this protein.

The gene sequence of presenilin-2 does not match any known human gene sequences, except for that of presenilin-1, and the identity and function of both these proteins remain speculative. This antiserum, has been shown to be immunoreactive to the presenilin-2 immunizing peptide by ELISA, but does not show immunoreactivity with the immunizing peptide used for the presenilin-1 antibody. Currently, this antibody has been shown to label neurons in both AD and normal control brains using frozen-fixed sections. This antibody should be a valuable tool for scientists who will be working to discover the function of this protein in normal individuals and its role in Alzheimer's disease.

References: Levy-Lahad, E., et al. Candidate gene for the chromosome 1 familial Alzheimer=s disease locus. Science 269: 973-977, 1995.


DATA SHEET: POLYCLONAL ANTISERUM

DESCRIPTION: Presenilin-2 Peptide Antiserum

PRODUCT NO: cat#RDI-PRESEN2abG    $312.00/0.25ml

LOT NO: K069HA

ANTIBODY HOST: Goat

IMMUNOGEN: Synthetic peptide corresponding to amino acids 31-47 of the N-terminus of the human Alzheimer's disease presenilin-2 protein (PS-2)

Sequence: C Q E G R Q G P E D G E N T A Q W  (cat#RDI-PRESEN2-CPX   $469.00/1mg)

FORM: Whole antiserum

CONCENTRATION: N/A

TITER: Recommended dilutions based on neat serum:

             ‚ ELISA using immunizing peptide: approx 1:20,000

             ‚ IHC formalin-fixed, paraffin sections: approx 1:300

              ‚ IHC paraformaldehyde-fixed, frozen sections: approx  1:1000

              ‚ Western blots: Not determined.

SPECIFICITY: Specific for residues 31-47 on the N-terminus of presenilin-2

STORAGE: 4° C short term; -20° long term. Recommend aliquoting and store 20 DEG C. avoid frequent freeze thaw cycles.

FOR RESEARCH AND MANUFACTURING USE ONLY


Goat Presenilin-2 Antiserum   Cat No. RDI-PRESEN2abG

Alzheimer disease brain showing immunohistochemical staining of neuron at 1:300 dilution in formalin-fixed, paraffin-embedded section of hippocampus.


 RDI Divison of researchd Industries Intl

San Jose, 95123 CA Snell ave 658

USA

or (800) 370-2222

EMAIL:margaret@cellular-research.com

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