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ANTIBODIES  

(anti-Human and others as indicated)

RDI Divison of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.


PGP9.5 (see below 1 monoclonal and 2 polyclonals)
Monoclonal anti-Protein Gene Product 9.5

cat# RDI-PGP95abm   $535.00/vial

Background: Protein gene product (PGP) 9.5 is a neuron specific protein, structurally and immunologically distinct from neuron specific enolase. The protein which has a molecular weight of 27kD was first identified by high resolution two dimensional PAGE. Standard immunohistochemical techniques have demonstrated the presence of PGP9.5 in neurons and nerve fibres al all levels of the central and peripheral nervous system, in many neuroendocrine cells, in segments of the renal tubules, in spermatogonia and Leydig cells of the testis, in ova and in some cells of both the pregnant and non-pregnant corpus luteum. PGP9.5 is known to be a member of the ubiquitin C terminal hydroxylase family and is also concentrated with inclusion bodies suggesting that such structures may be metabolically dynamic regions of the cells.

Specificity:* recognizes human protein gene product 9.5

Presentation: 1 ml lyophilized supernatant with 15mM sodium azide added.

Clone: 10A1

Isotype: mIgG2b

Antigen: Prokaryotic recombinant fusion protein corresponding to the full length of the protein gene product 9.5 molecule

Hybridoma: mouse myeloma (p3-NS1-Ag4-1)

USES: -acetone fixed frozen sections

            -Paraffin sections (using high temp unmasking technique, 0.01M citrate buffer pH 6.0, approx 10 minutes)

           -typical dilutions:1:20-1:40, 60 minutes incubation at 25 DEG C with ABC detection

           -western blot:1:500-1:1000

Stain: Cytoplasmic and nuclear

Controls: small intestine; enteric ganglion cells

Reconstitution: 1 ml distilled water. Allow to set 30-60 minutes, mix well.

Storage: Store lyophilized material at 4 DEG C. Store reconstituted material at 4 DEG C for immediate use. For long term use, recommend aliquoting and store at -20 DEG C. Avoid frequent freeze thaw cycles. Store in non frost free freezer.

general ref:

-Lowe J et al, Ubiquitin carboxy-terminal hydrolase (PGP 9.5) is selectively present in ubiquitinated inclusion bodies characteristic of human neurodegenerative diseases. journal of pathology 161:153-160 (1990)

-Wilkinson K D et al, The neuron specific protein PGP9.5 is a ubiquitin carboxyl-terminal hydrolase. Science 246:670-675 (1989)

-Wilson POG et al, The immunolocalization of protein gene product 9.5 using rabbit polyclonal and mouse monoclonal antibodies. Br.J.Exp. Path 69:91-104 (1988)

-Day I N M et al, Molecular cloning of cDNA coding for human PGP9.5 protein. A novel cytoplasmic marker for neurons and neuroendocrine cells FEBS Letters 210(2 157:160(1987)

-Thompson RJ et al. PGP9.5-a new marker for vertebrate and neuroendocrine cells. Brain Research 278:224-228 (1983)

FOR IN VITRO RESEARCH USE ONLY-NOT FOR USE IN DIAGNOSTICS


Rabbit anti-PGP9.5 (Human/Mouse/Rat/Pig)

cat# RDI-PGP95MabR      $375.00/50ul

       RDI-PGP95MabR-1   $812.00/150ul

Presentation: liquid whole serum with 0.05% sodium azide

Immunogen: 175-191 of soluble cytoplasmic protein of human PGP9.5 GASSEDTLLKDAAKVCL

Reactivity: human, mouse, and rat and pig (others not tested)

Use: -western blot: 1:2000 *   ( > with chemiluminescence detection)

           Histochemistry: approx 1:4000

           Optimal dilutions should be determined by user for each application

Storage: Store frozen. Aliquot as undiluted antisera and immediately place at -20 DEG C. At first thaw, tighten cap, centrifuge for 1-2 minutes at 1000 rpm to concentrate material in vial. Stable at least 6 months at -20 DEG C. Avoid repeated freeze/thaw cycles.

Ref: J Comp Neurol 380, 164-74 (1997)

For Research Use Only

Sample Protocol: Antiserum was used on perfusion fixed tissue. Perfusion 1)calcium-free Tyrode's solution 2) paraformaldehyde-picric acid fixative and 3) 10% sucrose in PBS as a cryo-protectant. Desired tissues were stored overnight in 10% sucrose in PBS. Slide mounted tissue sections were processed for indirect immunofluorescence. Slides were incubated with blocking buffer for 1 hour at room temp. Primary antiserum was diluted with blocking buffer to the appropriate working concentration. Blocking buffer was removed and slides were incubated for 18-24 hours at 4 DEG C with primary antiserum. Slides were rinsed 3 times and then incubated with secondary antibodies for 1 hour at room temp. Slides were again rinsed 3 times and coverslipped. Staining was examined using fluorescence microscopy.

-western blot:solubilized human brain extract was examined by SDS-PAGE (4-20% tris-glycine) under reducing conditions. The gel was transferred to a nitrocellulose membrane and blocked overnight at room temp with casein blocking buffer. Following blocking, primary antiserum diluted 1:2000 in 1% BSA,PBS) was added for 4 hours at room temp. Membranes were then washed and incubated with alkaline phosphatase conjugated secondary antibody for 45 minutes at room temp. After washing, the membranes were treated with BCIP/NBT substrate reagents to visualzie immobilized protein.


Guinea Pig anti-PGP9.5

cat# RDI-PGP95abGP        $375.00/50ul

       RDI-PGP95abGP-1     $750.00/150ul

Presentation: liquid whole serum with 0.05% sodium azide

Immunogen: Corresponding to residues 175-191 of soluble cytoplasmic protein of human PGP9.5 GASSEDTLLKDAAKVCL

Reactivity: human, mouse, and rat (others not tested)

Use: Histochemistry: approx 1:500 Optimal dilutions should be determined by user for each application

Storage: Store frozen. Aliquot as undiluted antisera and immediately place at -20 DEG C. At first thaw, tighten cap, centrifuge for 1-2 minutes at 1000 rpm to concentrate material in vial. Stable at least 6 months at -20 DEG C. Avoid repeated freeze/thaw cycles.

Ref: J Comp Neurol 380, 164-74 (1997)

For Research Use Only

Sample Protocol: Antiserum was used on perfusion fixed tissue.Perfusion 1)calcium-free Tyrode's solution 2) paraformaldehyde-picric acid fixative and 3) 10% sucrose in PBS as a cryoprotectant. Desired tissues were stored overnight in 10% sucrose in PBS.

Slide mounted tissue sections were processed for indirect immunofluorescence. Slides were incubated with blocking buffer for 1 hour at room temp. Primary antiserum was diluted with blocking buffer to the appropriate working concentration. Blocking buffer was removed and slides were incubated for 18-24 hours at 4 DEG C with primary antiserum. Slides were rinsed 3 times and then incubated with secondary antibodies for 1 hour at room temp. Slides were again rinsed 3 times and coverslipped. Staining was examined using fluorescence microscopy.

see anti-guinea pig secondary antibodies at: http://www.researchd.com/rdiabs/2ndabs.htm



RDI Divison of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

Ordering terms

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