rev: July 25, 2000
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(anti-Human and others as indicated)
RDI Divison of Fitzgerald Industries Intl offers a wide line of antibodies. Since no one antibody works best for all applications (neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.
Cat. No. RDI-PARKINabG $312.00/0.25ml
A goat antiserum to a synthetic peptide that corresponds to amino acids 83-97 of the N-terminus of the Parkinson disease Parkin protein is currently available. The Parkin gene was discovered on chromosome 6 and is responsible for an early onset form of autosomal recessive juvenile Parkinson disease. It shows moderate similarity to ubiquitin at the N-terminus and the gene is expressed in various regions of the brain, including the substantia nigra. Additionally, it is also expressed in other tissues, such as the heart, testes and skeletal muscle.
-The function of this gene product is currently unknown, but it is speculated
that this protein may function in the control of cell growth, differentiation
and development. This antiserum, has been shown to be immunoreactive to the
Parkin immunizing peptide by ELISA. This antibody has been shown to label
neurons in Parkinson disease brains using formalin-fixed, paraffin-embedded
sections and it labels a 65 kD protein in Western blots. This antibody should
be a valuable tool for scientists who will be working to discover the function
of this protein in normal individuals and its role in Parkinsons
-References: Kitada, T., et al. Mutations in the Parkin Gene Cause Autosomal Recessive Juvenile Parkinsonism. Nature 392: 605-608, 1998.
DATA SHEET: POLYCLONAL ANTISERUM
DESCRIPTION: Parkin N-terminal Peptide Antiserum
PRODUCT NO: RDI-PARKINabG
ANTIBODY HOST: Goat
IMMUNOGEN: Synthetic peptide corresponding to amino acids 83-97of the N-terminus of the human Parkin peptide
Sequence: T G G D D P R N A A G G C E R (cat#RDI-PARKIN-CPX $469.00/1mg)
FORM: Whole antiserum
TITER: Recommended dilutions based on neat serum:
-ELISA using immunizing peptide = 1:1,000
-IHC formalin-fixed, paraffin sections = 1:300
-Western Blots: 1:5,000
-IHC paraformaldehyde-fixed, frozen sections N/D
SPECIFICITY: Specific for residues 83-97 on the N-terminus of human Parkin
STORAGE: 4 DEG C short term; -20 DEG C long term
FOR RESEARCH AND MANUFACTURING USE ONLY
Parkinson disease brain showing Immunohistochemical staining of neurons at 1:300 dilution in formalin-fixed, paraffin-embedded section of substantia nigra.
Western blot of Parkinson disease brain using Parkin antibody at 1:5,000 dilution. Bands will vary depending upon degree of substitution with ubiquitin. Unmodified Parkin MW=approx 53Kda.
RDI Divison of Fitzgerald Industries Intl
34 Junction Square Drive
Concord MA 01742-3049
phone (978) 371-6446 or (800) 370-2222
fax (978) 371-2266
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