rev: July 25, 2000

HOME (index page)

Return ( alphabetical antibody index page)

Return  (neurohistology ab index page)


ANTIBODIES  

                                                                  (anti-Human and others as indicated)

RDI Divison of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.


PRODUCT: PARKIN N-TERMINAL PEPTIDE ANTISERUM

 

Cat. No. RDI-PARKINabG    $312.00/0.25ml

 

 A goat antiserum to a synthetic peptide that corresponds to amino acids 83-97 of the N-terminus of the Parkinson disease Parkin protein is currently available. The Parkin gene was discovered on chromosome 6 and is responsible for an early onset form of autosomal recessive juvenile Parkinson disease. It shows moderate similarity to ubiquitin at the N-terminus and the gene is expressed in various regions of the brain, including the substantia nigra. Additionally, it is also expressed in other tissues, such as the heart, testes and skeletal muscle.

-The function of this gene product is currently unknown, but it is speculated that this protein may function in the control of cell growth, differentiation and development. This antiserum, has been shown to be immunoreactive to the Parkin immunizing peptide by ELISA. This antibody has been shown to label neurons in Parkinson disease brains using formalin-fixed, paraffin-embedded sections and it labels a 65 kD protein in Western blots. This antibody should be a valuable tool for scientists who will be working to discover the function of this protein in normal individuals and its role in Parkinson’s disease.

-References: Kitada, T., et al. Mutations in the Parkin Gene Cause Autosomal Recessive Juvenile Parkinsonism. Nature 392: 605-608, 1998. 

 DATA SHEET: POLYCLONAL ANTISERUM

DESCRIPTION: Parkin N-terminal Peptide Antiserum

 

PRODUCT NO:   RDI-PARKINabG

 

LOT NO:

 

ANTIBODY HOST: Goat

 

IMMUNOGEN: Synthetic peptide corresponding to amino acids 83-97of the N-terminus of the human Parkin peptide

Sequence: T G G D D P R N A A G G C E R (cat#RDI-PARKIN-CPX $469.00/1mg)


 

FORM: Whole antiserum

 

CONCENTRATION: N/A

 

TITER: Recommended dilutions based on neat serum:
-ELISA using immunizing peptide = 1:1,000
-IHC formalin-fixed, paraffin sections = 1:300
-Western Blots: 1:5,000
-IHC paraformaldehyde-fixed, frozen sections N/D

 

SPECIFICITY: Specific for residues 83-97 on the N-terminus of human Parkin Protein
 

STORAGE: 4 DEG C short term; -20 DEG C long term

 

FOR RESEARCH AND MANUFACTURING USE ONLY

 

 Parkinson disease brain showing Immunohistochemical staining of neurons at 1:300 dilution in formalin-fixed, paraffin-embedded section of substantia nigra.

Western blot of Parkinson disease brain using Parkin antibody at 1:5,000 dilution. Bands will vary depending upon degree of substitution with ubiquitin. Unmodified Parkin MW=approx 53Kda.


 RDI Divison of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

RETURN(neurohistology ab page)

Return (alphabetical antibody page)